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Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes.
Genome. 2018 Jul; 61(7):515-521.G

Abstract

Synthesized oligonucleotides (oligos) can be used as effective probes similar to plasmid clones for chromosome identification in fluorescence in situ hybridization (FISH) analysis, making oligo FISH a simpler and more efficient molecular cytogenetic technique for studying plants. In this study, multiplex oligonucleotide probes, including pSc119.2-1, pAs1-4, (GAA)10, (AAC)6, and pTa71, were combined and used in FISH to identify chromosomes in common wheat, Thinopyrum intermedium, and a wheat - Th. intermedium amphiploid TE256-1. In comparison with general FISH probes, signals generated by the multiplex probes were more abundant, colorful, and characteristic. Combining the results of genomic in situ hybridization (GISH) with FISH, Th. intermedium chromosomes and alien chromosomes in TE256-1 could be classified and identified more precisely, especially the J- and Js-genome chromosomes. Moreover, based on the FISH results using multiplex probes, more structural variations in wheat chromosomes of TE256-1 were detected. The results indicated that multiplex oligo probes would have a wide range of application prospects in the creation and identification of wheat - Th. intermedium germplasms.

Authors+Show Affiliations

a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, Shandong 271018, China.b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, China.a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, Shandong 271018, China.a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, Shandong 271018, China. b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, China.c United States Department of Agriculture - Agricultural Research Services, Forage and Range Research Laboratory, Logan, UT 84322-6300, USA.b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, China.a State Key Laboratory of Crop Biology, Shandong Agriculture University, Tai'an, Shandong 271018, China. b College of Agronomy, Shandong Agriculture University, Tai'an, Shandong 271018, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29738283

Citation

Cui, Yu, et al. "Identification of Chromosomes in Thinopyrum Intermedium and Wheat Th. Intermedium Amphiploids Based On Multiplex Oligonucleotide Probes." Genome, vol. 61, no. 7, 2018, pp. 515-521.
Cui Y, Zhang Y, Qi J, et al. Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes. Genome. 2018;61(7):515-521.
Cui, Y., Zhang, Y., Qi, J., Wang, H., Wang, R. R., Bao, Y., & Li, X. (2018). Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes. Genome, 61(7), 515-521. https://doi.org/10.1139/gen-2018-0019
Cui Y, et al. Identification of Chromosomes in Thinopyrum Intermedium and Wheat Th. Intermedium Amphiploids Based On Multiplex Oligonucleotide Probes. Genome. 2018;61(7):515-521. PubMed PMID: 29738283.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of chromosomes in Thinopyrum intermedium and wheat Th. intermedium amphiploids based on multiplex oligonucleotide probes. AU - Cui,Yu, AU - Zhang,Yanping, AU - Qi,Juan, AU - Wang,Honggang, AU - Wang,Richard R-C, AU - Bao,Yinguang, AU - Li,Xingfeng, Y1 - 2018/05/08/ PY - 2018/5/9/pubmed PY - 2018/10/23/medline PY - 2018/5/9/entrez KW - Thinopyrum intermedium KW - chromosome variation KW - hybridation in situ KW - in situ hybridization KW - multiplex probes KW - sondes en multiplexe KW - variation chromosomique SP - 515 EP - 521 JF - Genome JO - Genome VL - 61 IS - 7 N2 - Synthesized oligonucleotides (oligos) can be used as effective probes similar to plasmid clones for chromosome identification in fluorescence in situ hybridization (FISH) analysis, making oligo FISH a simpler and more efficient molecular cytogenetic technique for studying plants. In this study, multiplex oligonucleotide probes, including pSc119.2-1, pAs1-4, (GAA)10, (AAC)6, and pTa71, were combined and used in FISH to identify chromosomes in common wheat, Thinopyrum intermedium, and a wheat - Th. intermedium amphiploid TE256-1. In comparison with general FISH probes, signals generated by the multiplex probes were more abundant, colorful, and characteristic. Combining the results of genomic in situ hybridization (GISH) with FISH, Th. intermedium chromosomes and alien chromosomes in TE256-1 could be classified and identified more precisely, especially the J- and Js-genome chromosomes. Moreover, based on the FISH results using multiplex probes, more structural variations in wheat chromosomes of TE256-1 were detected. The results indicated that multiplex oligo probes would have a wide range of application prospects in the creation and identification of wheat - Th. intermedium germplasms. SN - 1480-3321 UR - https://www.unboundmedicine.com/medline/citation/29738283/Identification_of_chromosomes_in_Thinopyrum_intermedium_and_wheat_Th__intermedium_amphiploids_based_on_multiplex_oligonucleotide_probes_ L2 - https://cdnsciencepub.com/doi/10.1139/gen-2018-0019?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -