TY - JOUR T1 - Boronic Acid Functionalized Au Nanoparticles for Selective MicroRNA Signal Amplification in Fiber-Optic Surface Plasmon Resonance Sensing System. AU - Qian,Siyu, AU - Lin,Ming, AU - Ji,Wei, AU - Yuan,Huizhen, AU - Zhang,Yang, AU - Jing,Zhenguo, AU - Zhao,Jianzhang, AU - Masson,Jean-François, AU - Peng,Wei, Y1 - 2018/05/15/ PY - 2018/5/10/pubmed PY - 2019/10/8/medline PY - 2018/5/10/entrez KW - boronic acid KW - fiber-optic SPR sensors KW - gold nanoparticles KW - microRNA detection KW - surface plasmon resonance (SPR) SP - 929 EP - 935 JF - ACS sensors JO - ACS Sens VL - 3 IS - 5 N2 - MicroRNA (miRNA) regulates gene expression and plays a fundamental role in multiple biological processes. However, if both single-stranded RNA and DNA can bind with capture DNA on the sensing surface, selectively amplifying the complementary RNA signal is still challenging for researchers. Fiber-optic surface plasmon resonance (SPR) sensors are small, accurate, and convenient tools for monitoring biological interaction. In this paper, we present a high sensitivity microRNA detection technique using phenylboronic acid functionalized Au nanoparticles (PBA-AuNPs) in fiber-optic SPR sensing systems. Due to the inherent difficulty directly detecting the hybridized RNA on the sensing surface, the PBA-AuNPs were used to selectively amplify the signal of target miRNA. The result shows that the method has high selectivity and sensitivity for miRNA, with a detection limit at 2.7 × 10-13 M (0.27 pM). This PBA-AuNPs amplification strategy is universally applicable for RNA detection with various sensing technologies, such as surface-enhanced Raman spectroscopy and electrochemistry, among others. SN - 2379-3694 UR - https://www.unboundmedicine.com/medline/citation/29741084/Boronic_Acid_Functionalized_Au_Nanoparticles_for_Selective_MicroRNA_Signal_Amplification_in_Fiber_Optic_Surface_Plasmon_Resonance_Sensing_System_ DB - PRIME DP - Unbound Medicine ER -