Tags

Type your tag names separated by a space and hit enter

Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae).
J Pharm Pharmacol. 2018 Aug; 70(8):1092-1101.JP

Abstract

OBJECTIVES

This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe).

METHODS

The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods.

KEY FINDINGS

AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml).

CONCLUSIONS

AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation.

Authors+Show Affiliations

Departamento de Histologia e Embriologia, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Histologia e Embriologia, Universidade Federal de Pernambuco, Recife, Brazil.Instituto Nacional do Semiárido (INSA), Campina Grande, Brazil.Instituto Nacional do Semiárido (INSA), Campina Grande, Brazil.Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brazil.Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brazil.Centro de Pesquisas Aggeu Magalhães (CPqAM), Fundação Oswaldo Cruz (FIOCRUZ), Recife, Brazil.Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Antibióticos, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Engenharia Biomédica, Universidade Federal de Pernambuco, Recife, Brazil.Departamento de Histologia e Embriologia, Universidade Federal de Pernambuco, Recife, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29744882

Citation

Santos, Dayane Kelly Dias do Nascimento, et al. "Evaluation of Cytotoxic, Immunomodulatory and Antibacterial Activities of Aqueous Extract From Leaves of Conocarpus Erectus Linnaeus (Combretaceae)." The Journal of Pharmacy and Pharmacology, vol. 70, no. 8, 2018, pp. 1092-1101.
Santos DKDDN, de Almeida VS, de Araujo DRC, et al. Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae). J Pharm Pharmacol. 2018;70(8):1092-1101.
Santos, D. K. D. D. N., de Almeida, V. S., de Araujo, D. R. C., Harand, W., Soares, A. K. A., Moreira, L. R., de Lorena, V. M. B., Magalhães, L. P. M., Ximenes, R. M., de Sena, K. X. D. F. R., de Melo, C. M. L., Napoleão, T. H., Lima, C. S. A., Yara, R., & Vieira, J. R. C. (2018). Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae). The Journal of Pharmacy and Pharmacology, 70(8), 1092-1101. https://doi.org/10.1111/jphp.12930
Santos DKDDN, et al. Evaluation of Cytotoxic, Immunomodulatory and Antibacterial Activities of Aqueous Extract From Leaves of Conocarpus Erectus Linnaeus (Combretaceae). J Pharm Pharmacol. 2018;70(8):1092-1101. PubMed PMID: 29744882.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae). AU - Santos,Dayane Kelly Dias do Nascimento, AU - de Almeida,Vanessa Silva, AU - de Araujo,Daniel Rodrigo Cavalcante, AU - Harand,Wolfgang, AU - Soares,Ana Karine de Araújo, AU - Moreira,Leyllane Rafael, AU - de Lorena,Virgínia Maria Barros, AU - Magalhães,Lucimeri Paulino Machado, AU - Ximenes,Rafael Matos, AU - de Sena,Kêsia Xisto da Fonseca Ribeiro, AU - de Melo,Cristiane Moutinho Lagos, AU - Napoleão,Thiago Henrique, AU - Lima,Cláudia Sampaio de Andrade, AU - Yara,Ricardo, AU - Vieira,Jeymesson Raphael Cardoso, Y1 - 2018/05/10/ PY - 2017/10/09/received PY - 2018/04/16/accepted PY - 2018/5/11/pubmed PY - 2018/11/1/medline PY - 2018/5/11/entrez KW - Conocarpus erectus KW - Staphylococcus aureus KW - Th1 response KW - lymphocyte activation KW - mangrove SP - 1092 EP - 1101 JF - The Journal of pharmacy and pharmacology JO - J. Pharm. Pharmacol. VL - 70 IS - 8 N2 - OBJECTIVES: This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe). METHODS: The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods. KEY FINDINGS: AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml). CONCLUSIONS: AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation. SN - 2042-7158 UR - https://www.unboundmedicine.com/medline/citation/29744882/Evaluation_of_cytotoxic_immunomodulatory_and_antibacterial_activities_of_aqueous_extract_from_leaves_of_Conocarpus_erectus_Linnaeus__Combretaceae__ L2 - https://doi.org/10.1111/jphp.12930 DB - PRIME DP - Unbound Medicine ER -