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MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6).
Med Sci Monit. 2018 May 10; 24:3056-3067.MS

Abstract

BACKGROUND

The dramatic increase of intervertebral disc degeneration (IDD) is considered to be a major cause of discogenic low back pain. The current study focused on the regulatory function of microRNA-194 (miR-194) on lipopolysaccharide (LPS)-induced inflammatory response in nucleus pulposus (NP) cells. MATERIAL AND

METHODS

LPS was used to treat NP cells to induce inflammatory responses. MiRNA and gene expression were detected by quantitative PCR. Proteins and protein expression levels were detected by Western blot and ELISA kit. Dual luciferase reporter assay was applied to identify the correlation between an miR-194- and TNF receptor-associated factor 6 (TRAF6) and to test NF-κB activity.

RESULTS

MiR-194 expression was reduced in LPS-induced NP cells. Both miR-194 overexpression and miR-194 inhibitor could regulate extracellular matrix (ECM) genes expression (Aggrecan and collagen II), MMP3, MMP13, ADAMTS4, and ADAMTS5, as well as inflammatory cytokines-associated genes (TNF-α, IL-1, IL-6, PGE2). Through a further study of the molecular mechanism, miR-194 was proved to be involved in the regulation of TRAF6 and its downstream signal molecule, nuclear factor-kappa B (NF-κB).

CONCLUSIONS

Finding of our study suggest that miR-194 can inhibit LPS-induced inflammatory response in NP cells of the intervertebral disc (IVD) by targeting TRAF6, which may contribute development of IDD biological therapy.

Authors+Show Affiliations

Department of Radiology, Yantai YEDA Hospital, Yantai, Shandong, China (mainland).Department of Orthopedics I, Yantai YEDA Hospital, Yantai, Shandong, China (mainland).Department of Radiology, Yantai YEDA Hospital, Yantai, Shandong, China (mainland).Department of Orthopedics I, Yantai YEDA Hospital, Yantai, Shandong, China (mainland).Department of Radiology, Yantai YEDA Hospital, Yantai, Shandong, China (mainland).

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29745371

Citation

Kong, Lili, et al. "MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc By Targeting TNF Receptor-Associated Factor 6 (TRAF6)." Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, vol. 24, 2018, pp. 3056-3067.
Kong L, Sun M, Jiang Z, et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit. 2018;24:3056-3067.
Kong, L., Sun, M., Jiang, Z., Li, L., & Lu, B. (2018). MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 3056-3067. https://doi.org/10.12659/MSM.907280
Kong L, et al. MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc By Targeting TNF Receptor-Associated Factor 6 (TRAF6). Med Sci Monit. 2018 May 10;24:3056-3067. PubMed PMID: 29745371.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - MicroRNA-194 Inhibits Lipopolysaccharide-Induced Inflammatory Response in Nucleus Pulposus Cells of the Intervertebral Disc by Targeting TNF Receptor-Associated Factor 6 (TRAF6). AU - Kong,Lili, AU - Sun,Mingxiao, AU - Jiang,Zhaofu, AU - Li,Liming, AU - Lu,Bing, Y1 - 2018/05/10/ PY - 2018/5/11/entrez PY - 2018/5/11/pubmed PY - 2018/9/25/medline SP - 3056 EP - 3067 JF - Medical science monitor : international medical journal of experimental and clinical research JO - Med. Sci. Monit. VL - 24 N2 - BACKGROUND The dramatic increase of intervertebral disc degeneration (IDD) is considered to be a major cause of discogenic low back pain. The current study focused on the regulatory function of microRNA-194 (miR-194) on lipopolysaccharide (LPS)-induced inflammatory response in nucleus pulposus (NP) cells. MATERIAL AND METHODS LPS was used to treat NP cells to induce inflammatory responses. MiRNA and gene expression were detected by quantitative PCR. Proteins and protein expression levels were detected by Western blot and ELISA kit. Dual luciferase reporter assay was applied to identify the correlation between an miR-194- and TNF receptor-associated factor 6 (TRAF6) and to test NF-κB activity. RESULTS MiR-194 expression was reduced in LPS-induced NP cells. Both miR-194 overexpression and miR-194 inhibitor could regulate extracellular matrix (ECM) genes expression (Aggrecan and collagen II), MMP3, MMP13, ADAMTS4, and ADAMTS5, as well as inflammatory cytokines-associated genes (TNF-α, IL-1, IL-6, PGE2). Through a further study of the molecular mechanism, miR-194 was proved to be involved in the regulation of TRAF6 and its downstream signal molecule, nuclear factor-kappa B (NF-κB). CONCLUSIONS Finding of our study suggest that miR-194 can inhibit LPS-induced inflammatory response in NP cells of the intervertebral disc (IVD) by targeting TRAF6, which may contribute development of IDD biological therapy. SN - 1643-3750 UR - https://www.unboundmedicine.com/medline/citation/29745371/MicroRNA_194_Inhibits_Lipopolysaccharide_Induced_Inflammatory_Response_in_Nucleus_Pulposus_Cells_of_the_Intervertebral_Disc_by_Targeting_TNF_Receptor_Associated_Factor_6__TRAF6__ L2 - https://www.medscimonit.com/download/index/idArt/907280 DB - PRIME DP - Unbound Medicine ER -