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Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran.
J Glob Antimicrob Resist. 2018 06; 13:197-200.JG

Abstract

OBJECTIVES

Fluoroquinolones (FQs) are recommended as the drugs of choice for the empirical treatment of urinary tract infections (UTIs). This study investigated the molecular determinants of FQ resistance in Escherichia coli and Klebsiella pneumoniae isolates in Iran.

METHODS

A total of 364 clinical isolates of E. coli (n=144) and K. pneumoniae (n=220) were collected from patients with UTI. Susceptibility of the isolates to ciprofloxacin, levofloxacin, gatifloxacin and nalidixic acid was evaluated by disk diffusion. The presence of qnrA, qnrB and qnrS genes was assessed by PCR. Nucleotide sequences of the gyrA and parC genes were determined.

RESULTS

Eighty-seven (60.4%) and 15 (6.8%) E. coli and K. pneumoniae isolates, respectively, were resistant to at least one of the tested FQs. Plasmid-mediated quinolone resistance (PMQR) genes were detected in 12.6% and 60.0% of FQ-resistant E. coli and K. pneumoniae, respectively. Whilst qnrB predominated in K. pneumoniae, qnrS was the most prevalent PMQR gene in E. coli. S83L (98.9%) and D87N (59.8%) were the most frequent mutations identified in GyrA of E. coli, and 55.2% (n=48) of FQ-resistant E. coli isolates had mutation in ParC harbouring S80I and E84V substitutions. The GyrAS83L substitution was found in only one FQ-resistant K. pneumoniae isolate.

CONCLUSIONS

FQ resistance was much more common in E. coli isolates than in K. pneumoniae. Whilst mutations in the drug target-encoding genes gyrA and parC were the major mechanisms involved in FQ resistance in E. coli, PMQR determinants commonly mediated FQ resistance in K. pneumoniae.

Authors+Show Affiliations

Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.Department of Basic Sciences, Islamic Azad University, Yasooj Branch, Yasooj, Iran.Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran; Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran. Electronic address: Khoramrooz@yums.ac.ir.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29747008

Citation

Mirzaii, Mehdi, et al. "Determination of gyrA and parC Mutations and Prevalence of Plasmid-mediated Quinolone Resistance Genes in Escherichia Coli and Klebsiella Pneumoniae Isolated From Patients With Urinary Tract Infection in Iran." Journal of Global Antimicrobial Resistance, vol. 13, 2018, pp. 197-200.
Mirzaii M, Jamshidi S, Zamanzadeh M, et al. Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran. J Glob Antimicrob Resist. 2018;13:197-200.
Mirzaii, M., Jamshidi, S., Zamanzadeh, M., Marashifard, M., Malek Hosseini, S. A. A., Haeili, M., Jahanbin, F., Mansouri, F., Darban-Sarokhalil, D., & Khoramrooz, S. S. (2018). Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran. Journal of Global Antimicrobial Resistance, 13, 197-200. https://doi.org/10.1016/j.jgar.2018.04.017
Mirzaii M, et al. Determination of gyrA and parC Mutations and Prevalence of Plasmid-mediated Quinolone Resistance Genes in Escherichia Coli and Klebsiella Pneumoniae Isolated From Patients With Urinary Tract Infection in Iran. J Glob Antimicrob Resist. 2018;13:197-200. PubMed PMID: 29747008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of gyrA and parC mutations and prevalence of plasmid-mediated quinolone resistance genes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection in Iran. AU - Mirzaii,Mehdi, AU - Jamshidi,Sanaz, AU - Zamanzadeh,Maryam, AU - Marashifard,Masoud, AU - Malek Hosseini,Seyed Ali Asghar, AU - Haeili,Mehri, AU - Jahanbin,Fariba, AU - Mansouri,Fariba, AU - Darban-Sarokhalil,Davood, AU - Khoramrooz,Seyed Sajjad, Y1 - 2018/05/07/ PY - 2018/01/06/received PY - 2018/04/26/revised PY - 2018/04/27/accepted PY - 2018/5/11/pubmed PY - 2019/9/21/medline PY - 2018/5/11/entrez KW - Escherichia coli KW - Klebsiella pneumoniae KW - PMQR genes KW - Plasmid-mediated quinolone resistance KW - gyrA KW - parC SP - 197 EP - 200 JF - Journal of global antimicrobial resistance JO - J Glob Antimicrob Resist VL - 13 N2 - OBJECTIVES: Fluoroquinolones (FQs) are recommended as the drugs of choice for the empirical treatment of urinary tract infections (UTIs). This study investigated the molecular determinants of FQ resistance in Escherichia coli and Klebsiella pneumoniae isolates in Iran. METHODS: A total of 364 clinical isolates of E. coli (n=144) and K. pneumoniae (n=220) were collected from patients with UTI. Susceptibility of the isolates to ciprofloxacin, levofloxacin, gatifloxacin and nalidixic acid was evaluated by disk diffusion. The presence of qnrA, qnrB and qnrS genes was assessed by PCR. Nucleotide sequences of the gyrA and parC genes were determined. RESULTS: Eighty-seven (60.4%) and 15 (6.8%) E. coli and K. pneumoniae isolates, respectively, were resistant to at least one of the tested FQs. Plasmid-mediated quinolone resistance (PMQR) genes were detected in 12.6% and 60.0% of FQ-resistant E. coli and K. pneumoniae, respectively. Whilst qnrB predominated in K. pneumoniae, qnrS was the most prevalent PMQR gene in E. coli. S83L (98.9%) and D87N (59.8%) were the most frequent mutations identified in GyrA of E. coli, and 55.2% (n=48) of FQ-resistant E. coli isolates had mutation in ParC harbouring S80I and E84V substitutions. The GyrAS83L substitution was found in only one FQ-resistant K. pneumoniae isolate. CONCLUSIONS: FQ resistance was much more common in E. coli isolates than in K. pneumoniae. Whilst mutations in the drug target-encoding genes gyrA and parC were the major mechanisms involved in FQ resistance in E. coli, PMQR determinants commonly mediated FQ resistance in K. pneumoniae. SN - 2213-7173 UR - https://www.unboundmedicine.com/medline/citation/29747008/Determination_of_gyrA_and_parC_mutations_and_prevalence_of_plasmid_mediated_quinolone_resistance_genes_in_Escherichia_coli_and_Klebsiella_pneumoniae_isolated_from_patients_with_urinary_tract_infection_in_Iran_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S2213-7165(18)30084-5 DB - PRIME DP - Unbound Medicine ER -