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A polyclonal antibody against extracellular loops 1 of chNHE1 blocks avian leukosis virus subgroup J infection.
Res Vet Sci. 2018 Jun; 118:477-483.RV

Abstract

Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and other various tumors, leading to great economical losses in poultry industry. It is a great challenge to develop effective preventive methods for ALV-J control due to its antigenic variations in the variable regions of envelope. In present study, we generated a mouse polyclonal antibody targeting the first extracellular loop (ECL1) of chicken Na+/H+ exchanger isoform 1 (chNHE1), the receptor of ALV-J, to block ALV-J infection in vitro and in vivo. In ALV-J infected DF-1 cells, chNHE1 expression and the intracellular pH (pHi) were up-regulated with "wave" pattern, indicating that the disequilibrium of ALV-J infected cells associated with chNHE1. Next, we validated that ALV-J infection was significantly blocked with time dependent after treating with anti-ECL1 antibody and accordingly the pHi value were recovered, indicating the blockage of ALV-J infection did not affect Na+/H+ exchange. Furthermore, in anti-ECL1 antibody treatment chickens that infected by ALV-J, weight gain and immune organs were recovered, and viral loads were significantly decreased, and the tissue injury and inflammation were reduced significantly from 21 to 35 days of age. The study demonstrated that anti-ECL1 antibody effectively blocks ALV-J infection without affecting Na+/H+ exchange, and sheds light on a novel strategy for retroviruses control.

Authors+Show Affiliations

College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. Electronic address: czqsd@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29747134

Citation

Meng, Wei, et al. "A Polyclonal Antibody Against Extracellular Loops 1 of chNHE1 Blocks Avian Leukosis Virus Subgroup J Infection." Research in Veterinary Science, vol. 118, 2018, pp. 477-483.
Meng W, Zhou D, Li C, et al. A polyclonal antibody against extracellular loops 1 of chNHE1 blocks avian leukosis virus subgroup J infection. Res Vet Sci. 2018;118:477-483.
Meng, W., Zhou, D., Li, C., Wang, G., Huang, L., & Cheng, Z. (2018). A polyclonal antibody against extracellular loops 1 of chNHE1 blocks avian leukosis virus subgroup J infection. Research in Veterinary Science, 118, 477-483. https://doi.org/10.1016/j.rvsc.2018.04.014
Meng W, et al. A Polyclonal Antibody Against Extracellular Loops 1 of chNHE1 Blocks Avian Leukosis Virus Subgroup J Infection. Res Vet Sci. 2018;118:477-483. PubMed PMID: 29747134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A polyclonal antibody against extracellular loops 1 of chNHE1 blocks avian leukosis virus subgroup J infection. AU - Meng,Wei, AU - Zhou,Defang, AU - Li,Chengui, AU - Wang,Guihua, AU - Huang,Libo, AU - Cheng,Ziqiang, Y1 - 2018/05/02/ PY - 2017/07/11/received PY - 2018/04/27/revised PY - 2018/04/30/accepted PY - 2018/5/11/pubmed PY - 2019/5/3/medline PY - 2018/5/11/entrez KW - Anti-ECL1 antibody KW - Avian leukosis virus subgroup J KW - Chicken Na(+)/H(+) exchanger isoform 1 SP - 477 EP - 483 JF - Research in veterinary science JO - Res. Vet. Sci. VL - 118 N2 - Avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, induces myelocytomas and other various tumors, leading to great economical losses in poultry industry. It is a great challenge to develop effective preventive methods for ALV-J control due to its antigenic variations in the variable regions of envelope. In present study, we generated a mouse polyclonal antibody targeting the first extracellular loop (ECL1) of chicken Na+/H+ exchanger isoform 1 (chNHE1), the receptor of ALV-J, to block ALV-J infection in vitro and in vivo. In ALV-J infected DF-1 cells, chNHE1 expression and the intracellular pH (pHi) were up-regulated with "wave" pattern, indicating that the disequilibrium of ALV-J infected cells associated with chNHE1. Next, we validated that ALV-J infection was significantly blocked with time dependent after treating with anti-ECL1 antibody and accordingly the pHi value were recovered, indicating the blockage of ALV-J infection did not affect Na+/H+ exchange. Furthermore, in anti-ECL1 antibody treatment chickens that infected by ALV-J, weight gain and immune organs were recovered, and viral loads were significantly decreased, and the tissue injury and inflammation were reduced significantly from 21 to 35 days of age. The study demonstrated that anti-ECL1 antibody effectively blocks ALV-J infection without affecting Na+/H+ exchange, and sheds light on a novel strategy for retroviruses control. SN - 1532-2661 UR - https://www.unboundmedicine.com/medline/citation/29747134/A_polyclonal_antibody_against_extracellular_loops_1_of_chNHE1_blocks_avian_leukosis_virus_subgroup_J_infection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0034-5288(17)30733-6 DB - PRIME DP - Unbound Medicine ER -