Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis.
Toxins (Basel). 2018 05 10; 10(5)T

Abstract

Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification.

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Authors+Show Affiliations

Roué M

Institut de Recherche pour le Développement (IRD)-UMR 241 EIO, PO box 53267, 98716 Pirae, Tahiti, French Polynesia. melanie.roue@ird.fr.

Darius HT

Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae-UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia. tdarius@ilm.pf.

Ung A

Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae-UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia. aung@ilm.pf.

Viallon J

Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae-UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia. jviallon@ilm.pf.

Sibat M

IFREMER, Phycotoxins Laboratory, F-44311 Nantes CEDEX, France. manoella.sibat@ifremer.fr.

Hess P

IFREMER, Phycotoxins Laboratory, F-44311 Nantes CEDEX, France. philipp.hess@ifremer.fr.

Amzil Z

IFREMER, Phycotoxins Laboratory, F-44311 Nantes CEDEX, France. zouher.amzil@ifremer.fr.

Chinain M

Institut Louis Malardé (ILM), Laboratory of Toxic Microalgae-UMR 241-EIO, PO Box 30, 98713 Papeete, Tahiti, French Polynesia. mchinain@ilm.pf.

MeSH

AnimalsBivalviaCell Line, TumorCiguatoxinsDietDinoflagellidaMiceTissue Distribution

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29747460

Citation

Roué, Mélanie, et al. "Tissue Distribution and Elimination of Ciguatoxins in Tridacna Maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus Polynesiensis." Toxins, vol. 10, no. 5, 2018.

Roué M, Darius HT, Ung A, et al. Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. Toxins (Basel). 2018;10(5).

Roué, M., Darius, H. T., Ung, A., Viallon, J., Sibat, M., Hess, P., Amzil, Z., & Chinain, M. (2018). Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. Toxins, 10(5). https://doi.org/10.3390/toxins10050189

Roué M, et al. Tissue Distribution and Elimination of Ciguatoxins in Tridacna Maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus Polynesiensis. Toxins (Basel). 2018 05 10;10(5) PubMed PMID: 29747460.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. AU - Roué,Mélanie, AU - Darius,Hélène Taiana, AU - Ung,André, AU - Viallon,Jérôme, AU - Sibat,Manoella, AU - Hess,Philipp, AU - Amzil,Zouher, AU - Chinain,Mireille, Y1 - 2018/05/10/ PY - 2018/04/17/received PY - 2018/05/03/revised PY - 2018/05/07/accepted PY - 2018/5/12/entrez PY - 2018/5/12/pubmed PY - 2019/3/20/medline KW - CBA-N2a KW - Gambierdiscus polynesiensis KW - LC-MS/MS KW - anatomical distribution KW - ciguatoxins KW - ex situ exposure to toxic algae KW - giant clams KW - toxin elimination JF - Toxins JO - Toxins (Basel) VL - 10 IS - 5 N2 - Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/29747460/Tissue_Distribution_and_Elimination_of_Ciguatoxins_in_Tridacna_maxima__Tridacnidae_Bivalvia__Fed_Gambierdiscus_polynesiensis_ DB - PRIME DP - Unbound Medicine ER -

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Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis.Toxins (Basel). 2018 05 10; 10(5)T