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Extraction of pectin from ponkan (Citrus reticulata Blanco cv. Ponkan) peel: Optimization and structural characterization.
Int J Biol Macromol. 2018 Oct 01; 117:385-391.IJ

Abstract

A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel. The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time. The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g. The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value. PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans. In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering. The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated.

Authors+Show Affiliations

Federal University of Parana, Department of Biochemistry and Molecular Biology, PO Box 19046, 81531-980 Curitiba, Parana, Brazil.Federal University of Parana, Department of Biochemistry and Molecular Biology, PO Box 19046, 81531-980 Curitiba, Parana, Brazil.Federal University of Viçosa, Department of Chemistry, 36570-000 Viçosa, Minas Gerais, Brazil.Federal University of Parana, Department of Biochemistry and Molecular Biology, PO Box 19046, 81531-980 Curitiba, Parana, Brazil. Electronic address: clop@ufpr.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29753767

Citation

Colodel, Cristiane, et al. "Extraction of Pectin From Ponkan (Citrus Reticulata Blanco Cv. Ponkan) Peel: Optimization and Structural Characterization." International Journal of Biological Macromolecules, vol. 117, 2018, pp. 385-391.
Colodel C, Vriesmann LC, Teófilo RF, et al. Extraction of pectin from ponkan (Citrus reticulata Blanco cv. Ponkan) peel: Optimization and structural characterization. Int J Biol Macromol. 2018;117:385-391.
Colodel, C., Vriesmann, L. C., Teófilo, R. F., & de Oliveira Petkowicz, C. L. (2018). Extraction of pectin from ponkan (Citrus reticulata Blanco cv. Ponkan) peel: Optimization and structural characterization. International Journal of Biological Macromolecules, 117, 385-391. https://doi.org/10.1016/j.ijbiomac.2018.05.048
Colodel C, et al. Extraction of Pectin From Ponkan (Citrus Reticulata Blanco Cv. Ponkan) Peel: Optimization and Structural Characterization. Int J Biol Macromol. 2018 Oct 1;117:385-391. PubMed PMID: 29753767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extraction of pectin from ponkan (Citrus reticulata Blanco cv. Ponkan) peel: Optimization and structural characterization. AU - Colodel,Cristiane, AU - Vriesmann,Lúcia Cristina, AU - Teófilo,Reinaldo Francisco, AU - de Oliveira Petkowicz,Carmen Lúcia, Y1 - 2018/06/01/ PY - 2018/01/21/received PY - 2018/04/12/revised PY - 2018/05/10/accepted PY - 2018/5/14/pubmed PY - 2018/11/10/medline PY - 2018/5/14/entrez KW - Pectin KW - Response surface methodology KW - Structural characterization SP - 385 EP - 391 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 117 N2 - A central composite experimental design was used to evaluate the influence of pH, extraction time and liquid:solid ratio on the yield and uronic acid content of the pectin from ponkan peel. The response surface methodology showed that the yield is positively influenced by lower pHs, longer extraction times and higher liquid:solid ratio, whereas the uronic acid content decreases with increasing extraction time. The conditions that resulted in the highest yield and highest uronic acid content were defined as pH 1.6, extraction time of 100 min and liquid:solid ratio of 36 mL/g. The pectin obtained under these conditions (PPOP) had an experimental yield of 25.6%, below the predicted theoretical value despite the good fit of the model (R2 = 0.96) and the galacturonic acid content was 84.5%, in close agreement with the predicted theoretical value. PPOP was composed mainly of a homogalacturonan with degree of methyl esterification of 85.7% and a rhamnogalacturonan I region mainly branched by galactans. In addition, PPOP had a very low degree of acetylation (0.1%) and average molar mass of 80,650 g/mol, determined by light scattering. The results showed that ponkan peel may be used as a source of citrus pectin in the regions where this species is cultivated. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/29753767/Extraction_of_pectin_from_ponkan__Citrus_reticulata_Blanco_cv__Ponkan__peel:_Optimization_and_structural_characterization_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(18)30373-8 DB - PRIME DP - Unbound Medicine ER -