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[Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells].
Wei Sheng Wu Xue Bao. 2017 Apr 04; 57(4):500-12.WS

Abstract

Objective

To study the function and mechanism of STM14_3514 gene that encoded in Salmonella pathogenicity island (SPI)-1 of Salmonella enterica serovar Typhimurium strain ATCC 14028.

Methods

We constructed STM14_3514 mutant strain and a complemented strain of the mutant. Through mice experiment, attachment assays, invasion assays, macrophage replication assays, western blot, and Quantitative real-time PCR analysis (qRT-PCR), we compared the virulence of the mutant strain to that of the wild-type 14028.

Results

STM14_3514 mutant shows increased virulence to mice, and the bacterial number of STM14_3514 mutant in liver, spleen, and ileum was more abundant than that of the wild-type strain. The increased virulence of STM14_3514 mutant is caused by its elevated invasion ability to epithelial cells (>2-fold and P<0.05). qRT-PCR and western blot results show that STM14_3514 reduced the expression of HilA and another SPI-1invasion locus. Moreover, the repression of HilA by STM14_3514 is mediated by HilC.

Conclusion

STM14_3514 is a negative regulator in SPI-1, which can repress HilA and SPI-1invasion locus through HilC, and possibly contribute to the repression on SPI-1 after bacterial invasion.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29756734

Citation

Jiang, Lingyan, et al. "[Putative Regulatory Protein STM14_3514 Decreases Salmonella Typhimurium Invasion of Epithelial Cells]." Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, vol. 57, no. 4, 2017, pp. 500-12.
Jiang L, Zhou Q, Wang P, et al. [Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells]. Wei Sheng Wu Xue Bao. 2017;57(4):500-12.
Jiang, L., Zhou, Q., Wang, P., Jiang, X., & Feng, L. (2017). [Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells]. Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica, 57(4), 500-12.
Jiang L, et al. [Putative Regulatory Protein STM14_3514 Decreases Salmonella Typhimurium Invasion of Epithelial Cells]. Wei Sheng Wu Xue Bao. 2017 Apr 4;57(4):500-12. PubMed PMID: 29756734.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Putative regulatory protein STM14_3514 decreases Salmonella Typhimurium invasion of epithelial cells]. AU - Jiang,Lingyan, AU - Zhou,Qixing, AU - Wang,Peisheng, AU - Jiang,Xiaohan, AU - Feng,Lu, PY - 2018/5/15/entrez PY - 2018/5/15/pubmed PY - 2018/7/6/medline SP - 500 EP - 12 JF - Wei sheng wu xue bao = Acta microbiologica Sinica JO - Wei Sheng Wu Xue Bao VL - 57 IS - 4 N2 - Objective: To study the function and mechanism of STM14_3514 gene that encoded in Salmonella pathogenicity island (SPI)-1 of Salmonella enterica serovar Typhimurium strain ATCC 14028. Methods: We constructed STM14_3514 mutant strain and a complemented strain of the mutant. Through mice experiment, attachment assays, invasion assays, macrophage replication assays, western blot, and Quantitative real-time PCR analysis (qRT-PCR), we compared the virulence of the mutant strain to that of the wild-type 14028. Results: STM14_3514 mutant shows increased virulence to mice, and the bacterial number of STM14_3514 mutant in liver, spleen, and ileum was more abundant than that of the wild-type strain. The increased virulence of STM14_3514 mutant is caused by its elevated invasion ability to epithelial cells (>2-fold and P<0.05). qRT-PCR and western blot results show that STM14_3514 reduced the expression of HilA and another SPI-1invasion locus. Moreover, the repression of HilA by STM14_3514 is mediated by HilC. Conclusion: STM14_3514 is a negative regulator in SPI-1, which can repress HilA and SPI-1invasion locus through HilC, and possibly contribute to the repression on SPI-1 after bacterial invasion. SN - 0001-6209 UR - https://www.unboundmedicine.com/medline/citation/29756734/[Putative_regulatory_protein_STM14_3514_decreases_Salmonella_Typhimurium_invasion_of_epithelial_cells]_ DB - PRIME DP - Unbound Medicine ER -