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Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte.
PLoS One. 2018; 13(5):e0197059.Plos

Abstract

The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control.

Authors+Show Affiliations

Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.Monsanto Co., 700 Chesterfield Parkway West, Chesterfield, Missouri, United States of America.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29758046

Citation

Khajuria, Chitvan, et al. "Development and Characterization of the First dsRNA-resistant Insect Population From Western Corn Rootworm, Diabrotica Virgifera Virgifera LeConte." PloS One, vol. 13, no. 5, 2018, pp. e0197059.
Khajuria C, Ivashuta S, Wiggins E, et al. Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte. PLoS One. 2018;13(5):e0197059.
Khajuria, C., Ivashuta, S., Wiggins, E., Flagel, L., Moar, W., Pleau, M., Miller, K., Zhang, Y., Ramaseshadri, P., Jiang, C., Hodge, T., Jensen, P., Chen, M., Gowda, A., McNulty, B., Vazquez, C., Bolognesi, R., Haas, J., Head, G., & Clark, T. (2018). Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte. PloS One, 13(5), e0197059. https://doi.org/10.1371/journal.pone.0197059
Khajuria C, et al. Development and Characterization of the First dsRNA-resistant Insect Population From Western Corn Rootworm, Diabrotica Virgifera Virgifera LeConte. PLoS One. 2018;13(5):e0197059. PubMed PMID: 29758046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and characterization of the first dsRNA-resistant insect population from western corn rootworm, Diabrotica virgifera virgifera LeConte. AU - Khajuria,Chitvan, AU - Ivashuta,Sergey, AU - Wiggins,Elizabeth, AU - Flagel,Lex, AU - Moar,William, AU - Pleau,Michael, AU - Miller,Kaylee, AU - Zhang,Yuanji, AU - Ramaseshadri,Parthasarathy, AU - Jiang,Changjian, AU - Hodge,Tracey, AU - Jensen,Peter, AU - Chen,Mao, AU - Gowda,Anilkumar, AU - McNulty,Brian, AU - Vazquez,Cara, AU - Bolognesi,Renata, AU - Haas,Jeffrey, AU - Head,Graham, AU - Clark,Thomas, Y1 - 2018/05/14/ PY - 2018/01/02/received PY - 2018/04/25/accepted PY - 2018/5/15/entrez PY - 2018/5/15/pubmed PY - 2018/8/1/medline SP - e0197059 EP - e0197059 JF - PloS one JO - PLoS One VL - 13 IS - 5 N2 - The use of dsRNA to control insect pests via the RNA interference (RNAi) pathway is being explored by researchers globally. However, with every new class of insect control compounds, the evolution of insect resistance needs to be considered, and understanding resistance mechanisms is essential in designing durable technologies and effective resistance management strategies. To gain insight into insect resistance to dsRNA, a field screen with subsequent laboratory selection was used to establish a population of DvSnf7 dsRNA-resistant western corn rootworm, Diabrotica virgifera virgifera, a major maize insect pest. WCR resistant to ingested DvSnf7 dsRNA had impaired luminal uptake and resistance was not DvSnf7 dsRNA-specific, as indicated by cross resistance to all other dsRNAs tested. No resistance to the Bacillus thuringiensis Cry3Bb1 protein was observed. DvSnf7 dsRNA resistance was inherited recessively, located on a single locus, and autosomal. Together these findings will provide insights for dsRNA deployment for insect pest control. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/29758046/Development_and_characterization_of_the_first_dsRNA_resistant_insect_population_from_western_corn_rootworm_Diabrotica_virgifera_virgifera_LeConte_ DB - PRIME DP - Unbound Medicine ER -