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An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones.
Methods Mol Biol. 2018; 1778:183-192.MM

Abstract

The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).

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Publisher Full Text (DOI)

Authors+Show Affiliations

Novák O
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic. novako@ueb.cas.cz. Faculty of Science, Palacký University, Olomouc, Czech Republic. novako@ueb.cas.cz.
Floková K
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, Olomouc, Czech Republic. Faculty of Science, Palacký University, Olomouc, Czech Republic. Plant Hormone Biology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.

MeSH

Abscisic AcidChromatography, High Pressure LiquidCyclopentanesIndoleacetic AcidsOxylipinsPlant Growth RegulatorsSalicylic AcidTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29761439

Citation

Novák, Ondřej, and Kristýna Floková. "An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones." Methods in Molecular Biology (Clifton, N.J.), vol. 1778, 2018, pp. 183-192.
Novák O, Floková K. An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones. Methods Mol Biol. 2018;1778:183-192.
Novák, O., & Floková, K. (2018). An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones. Methods in Molecular Biology (Clifton, N.J.), 1778, 183-192. https://doi.org/10.1007/978-1-4939-7819-9_13
Novák O, Floková K. An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones. Methods Mol Biol. 2018;1778:183-192. PubMed PMID: 29761439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones. AU - Novák,Ondřej, AU - Floková,Kristýna, PY - 2018/5/16/entrez PY - 2018/5/16/pubmed PY - 2019/2/14/medline KW - Abscisic acid KW - Auxins KW - Jasmonates KW - LC-MS KW - Liquid chromatography–mass spectrometry KW - Phytohormones KW - Salicylic acid KW - Solid-phase extraction (SPE) SP - 183 EP - 192 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1778 N2 - The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/29761439/An_UHPLC_MS/MS_Method_for_Target_Profiling_of_Stress_Related_Phytohormones_ DB - PRIME DP - Unbound Medicine ER -
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