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Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3.
Acta Pharmacol Sin. 2018 Dec; 39(12):1885-1893.AP

Abstract

Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G2/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P < 0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P < 0.05) and the increase of apoptosis index (P < 0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA database, we revealed that the overexpression of galectin-3 was associated with high tumor grade with lymph node metastasis, poor overall survival in UBC patients. Considering the remarkable inhibitory effects of MCP on UBC cell proliferation and survival in vitro and in vivo mainly through galectin-3, which is upregulated in UBCs, MCP may become an attractive agent, as a natural dietary fiber, for prevention and therapy of UBCs.

Authors+Show Affiliations

Department of Comparative Medicine, Jinling Hospital, Clinical School of Medical College of Nanjing University, Nanjing, 210002, China.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. University of Chinese Academy of Sciences, Beijing, 100049, China.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. University of Chinese Academy of Sciences, Beijing, 100049, China. Shanghai Tech University, Shanghai, 201210, China.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, 210061, China.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, 210061, China.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. Shanghai University, Shanghai, 200444, China.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. University of Chinese Academy of Sciences, Beijing, 100049, China. Shanghai Tech University, Shanghai, 201210, China.Department of Comparative Medicine, Jinling Hospital, Clinical School of Medical College of Nanjing University, Nanjing, 210002, China. yunshifeng1@163.com.State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center of Nanjing University, Nanjing, 210061, China. yanjun@nju.edu.cn. Collaborative Innovation Center of Genetics and Development, Shanghai, 200438, China. yanjun@nju.edu.cn.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China. rmhuang@simm.ac.cn. University of Chinese Academy of Sciences, Beijing, 100049, China. rmhuang@simm.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29769742

Citation

Fang, Tian, et al. "Modified Citrus Pectin Inhibited Bladder Tumor Growth Through Downregulation of Galectin-3." Acta Pharmacologica Sinica, vol. 39, no. 12, 2018, pp. 1885-1893.
Fang T, Liu DD, Ning HM, et al. Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3. Acta Pharmacol Sin. 2018;39(12):1885-1893.
Fang, T., Liu, D. D., Ning, H. M., Dan Liu, ., Sun, J. Y., Huang, X. J., Dong, Y., Geng, M. Y., Yun, S. F., Yan, J., & Huang, R. M. (2018). Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3. Acta Pharmacologica Sinica, 39(12), 1885-1893. https://doi.org/10.1038/s41401-018-0004-z
Fang T, et al. Modified Citrus Pectin Inhibited Bladder Tumor Growth Through Downregulation of Galectin-3. Acta Pharmacol Sin. 2018;39(12):1885-1893. PubMed PMID: 29769742.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3. AU - Fang,Tian, AU - Liu,Dan-Dan, AU - Ning,He-Ming, AU - Dan Liu,, AU - Sun,Jing-Ya, AU - Huang,Xiao-Jing, AU - Dong,Yu, AU - Geng,Mei-Yu, AU - Yun,Shi-Feng, AU - Yan,Jun, AU - Huang,Rui-Min, Y1 - 2018/05/16/ PY - 2017/09/25/received PY - 2018/01/08/accepted PY - 2018/01/07/revised PY - 2018/5/18/pubmed PY - 2019/1/5/medline PY - 2018/5/18/entrez KW - cell survival KW - galectin-3 KW - modified citrus pectin KW - urinary bladder cancer KW - xenograft SP - 1885 EP - 1893 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 39 IS - 12 N2 - Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G2/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P < 0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P < 0.05) and the increase of apoptosis index (P < 0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA database, we revealed that the overexpression of galectin-3 was associated with high tumor grade with lymph node metastasis, poor overall survival in UBC patients. Considering the remarkable inhibitory effects of MCP on UBC cell proliferation and survival in vitro and in vivo mainly through galectin-3, which is upregulated in UBCs, MCP may become an attractive agent, as a natural dietary fiber, for prevention and therapy of UBCs. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/29769742/Modified_citrus_pectin_inhibited_bladder_tumor_growth_through_downregulation_of_galectin_3_ L2 - https://doi.org/10.1038/s41401-018-0004-z DB - PRIME DP - Unbound Medicine ER -