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Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain.
Neural Plast. 2018; 2018:9347696.NP

Abstract

Despite decades of studies, the currently available drugs largely fail to control neuropathic pain. Koumine-an alkaloidal constituent derived from the medicinal plant Gelsemium elegans Benth.-has been shown to possess analgesic and anti-inflammatory properties; however, the underlying mechanisms remain unclear. In this study, we aimed to investigate the analgesic and anti-inflammatory effects and the possible underlying mechanisms of koumine. The analgesic and anti-inflammatory effects of koumine were explored by using chronic constriction injury of the sciatic nerve (CCI) neuropathic pain model in vivo and LPS-induced injury in microglia BV2 cells in vitro. Immunofluorescence staining and Western blot analysis were used to assess the modulator effect of koumine on microglia and astrocyte activation after CCI surgery. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of proinflammatory cytokines. Western blot analysis and quantitative real-time polymerase chain reaction (qPCR) were used to examine the modulator effect of koumine on microglial M1 polarization. We found that single or repeated treatment of koumine can significantly reduce neuropathic pain after nerve injury. Moreover, koumine showed inhibitory effects on CCI-evoked microglia and astrocyte activation and reduced proinflammatory cytokine production in the spinal cord in rat CCI models. In BV2 cells, koumine significantly inhibited microglia M1 polarization. Furthermore, the analgesic effect of koumine was inhibited by a TSPO antagonist PK11195. These findings suggest that the analgesic effects of koumine on CCI-induced neuropathic pain may result from the inhibition of microglia activation and M1 polarization as well as the activation of astrocytes while sparing the anti-inflammatory responses to neuropathic pain.

Authors+Show Affiliations

Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, Fujian, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Department of Pharmacy, Quanzhou Medical College, Quanzhou, Fujian 362100, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, Fujian, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, Fujian, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China.Department of Pharmacology and College of Pharmacy, Fujian Medical University, Fuzhou, Fujian 350004, China. Fujian Key Laboratory of Natural Medicine Pharmacology, College of Pharmacy, Fujian Medical University, Fuzhou, Fujian, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29770147

Citation

Jin, Gui-Lin, et al. "Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain." Neural Plasticity, vol. 2018, 2018, p. 9347696.
Jin GL, He SD, Lin SM, et al. Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain. Neural Plast. 2018;2018:9347696.
Jin, G. L., He, S. D., Lin, S. M., Hong, L. M., Chen, W. Q., Xu, Y., Yang, J., Li, S. P., & Yu, C. X. (2018). Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain. Neural Plasticity, 2018, 9347696. https://doi.org/10.1155/2018/9347696
Jin GL, et al. Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain. Neural Plast. 2018;2018:9347696. PubMed PMID: 29770147.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Koumine Attenuates Neuroglia Activation and Inflammatory Response to Neuropathic Pain. AU - Jin,Gui-Lin, AU - He,Sai-Di, AU - Lin,Shao-Mei, AU - Hong,Li-Mian, AU - Chen,Wan-Qing, AU - Xu,Ying, AU - Yang,Jian, AU - Li,Su-Ping, AU - Yu,Chang-Xi, Y1 - 2018/03/25/ PY - 2017/08/10/received PY - 2017/10/24/revised PY - 2018/02/13/accepted PY - 2018/5/18/entrez PY - 2018/5/18/pubmed PY - 2018/11/27/medline SP - 9347696 EP - 9347696 JF - Neural plasticity JO - Neural Plast VL - 2018 N2 - Despite decades of studies, the currently available drugs largely fail to control neuropathic pain. Koumine-an alkaloidal constituent derived from the medicinal plant Gelsemium elegans Benth.-has been shown to possess analgesic and anti-inflammatory properties; however, the underlying mechanisms remain unclear. In this study, we aimed to investigate the analgesic and anti-inflammatory effects and the possible underlying mechanisms of koumine. The analgesic and anti-inflammatory effects of koumine were explored by using chronic constriction injury of the sciatic nerve (CCI) neuropathic pain model in vivo and LPS-induced injury in microglia BV2 cells in vitro. Immunofluorescence staining and Western blot analysis were used to assess the modulator effect of koumine on microglia and astrocyte activation after CCI surgery. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate the levels of proinflammatory cytokines. Western blot analysis and quantitative real-time polymerase chain reaction (qPCR) were used to examine the modulator effect of koumine on microglial M1 polarization. We found that single or repeated treatment of koumine can significantly reduce neuropathic pain after nerve injury. Moreover, koumine showed inhibitory effects on CCI-evoked microglia and astrocyte activation and reduced proinflammatory cytokine production in the spinal cord in rat CCI models. In BV2 cells, koumine significantly inhibited microglia M1 polarization. Furthermore, the analgesic effect of koumine was inhibited by a TSPO antagonist PK11195. These findings suggest that the analgesic effects of koumine on CCI-induced neuropathic pain may result from the inhibition of microglia activation and M1 polarization as well as the activation of astrocytes while sparing the anti-inflammatory responses to neuropathic pain. SN - 1687-5443 UR - https://www.unboundmedicine.com/medline/citation/29770147/Koumine_Attenuates_Neuroglia_Activation_and_Inflammatory_Response_to_Neuropathic_Pain_ L2 - https://doi.org/10.1155/2018/9347696 DB - PRIME DP - Unbound Medicine ER -