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Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators.
Microb Cell Fact. 2018 May 17; 17(1):75.MC

Abstract

BACKGROUND

Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance.

RESULTS

Two sets of novel minimal transcriptional activators (DBDace2-VP16 and DBDcre1-VP16) were designed and expressed in T. reesei RUT C30. Expression of DBDace2-VP16 and DBDcre1-VP16 improved cellulase production under induction (avicel or lactose) and repression (glucose) conditions, respectively. The strain TMTA66 under avicel and TMTA139 under glucose with the highest cellulase activities outperformed other transformants and the parental strain under the corresponding conditions. For TMTA66 strains, the highest FPase activity was approximately 1.3-fold greater than that of the parental strain RUT C30 at 120 h of cultivation in a shake flask using avicel as the sole carbon source. The FPase activity (U/mg biomass) in TMTA139 strains was approximately 26.5-fold higher than that of the parental strain RUT C30 at 72 h of cultivation in a shake flask using glucose as the sole carbon source. Furthermore, the crude enzymes produced in the 7-L fermenter from TMTA66 and TMTA139 supplemented with commercial β-glucosidase hydrolyzed pretreated corn stover effectively.

CONCLUSIONS

These results show that replacing natural transcription factors with minimal transcriptional activators is a powerful strategy to enhance cellulase production in T. reesei. Our current study also offers an alternative genetic engineering strategy for the enhanced production of industrial products by other fungi.

Authors+Show Affiliations

State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China. wadexp@ecust.edu.cn.State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, 130 Meilong Road, P.O.B. 311, Shanghai, 200237, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29773074

Citation

Zhang, Jiajia, et al. "Enhanced Cellulase Production in Trichoderma Reesei RUT C30 Via Constitution of Minimal Transcriptional Activators." Microbial Cell Factories, vol. 17, no. 1, 2018, p. 75.
Zhang J, Zhang G, Wang W, et al. Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators. Microb Cell Fact. 2018;17(1):75.
Zhang, J., Zhang, G., Wang, W., Wang, W., & Wei, D. (2018). Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators. Microbial Cell Factories, 17(1), 75. https://doi.org/10.1186/s12934-018-0926-7
Zhang J, et al. Enhanced Cellulase Production in Trichoderma Reesei RUT C30 Via Constitution of Minimal Transcriptional Activators. Microb Cell Fact. 2018 May 17;17(1):75. PubMed PMID: 29773074.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators. AU - Zhang,Jiajia, AU - Zhang,Guoxiu, AU - Wang,Wei, AU - Wang,Wei, AU - Wei,Dongzhi, Y1 - 2018/05/17/ PY - 2018/03/10/received PY - 2018/05/11/accepted PY - 2018/5/19/entrez PY - 2018/5/19/pubmed PY - 2018/10/3/medline KW - ACEII KW - CREI KW - Cellulase production KW - Minimal transcriptional activator KW - Trichoderma reesei SP - 75 EP - 75 JF - Microbial cell factories JO - Microb. Cell Fact. VL - 17 IS - 1 N2 - BACKGROUND: Cellulase can convert lignocellulosic feedstocks into fermentable sugars, which can be used for the industrial production of biofuels and chemicals. The high cost of cellulase production remains a challenge for lignocellulose breakdown. Trichoderma reesei RUT C30 serves as a well-known industrial workhorse for cellulase production. Therefore, the enhancement of cellulase production by T. reesei RUT C30 is of great importance. RESULTS: Two sets of novel minimal transcriptional activators (DBDace2-VP16 and DBDcre1-VP16) were designed and expressed in T. reesei RUT C30. Expression of DBDace2-VP16 and DBDcre1-VP16 improved cellulase production under induction (avicel or lactose) and repression (glucose) conditions, respectively. The strain TMTA66 under avicel and TMTA139 under glucose with the highest cellulase activities outperformed other transformants and the parental strain under the corresponding conditions. For TMTA66 strains, the highest FPase activity was approximately 1.3-fold greater than that of the parental strain RUT C30 at 120 h of cultivation in a shake flask using avicel as the sole carbon source. The FPase activity (U/mg biomass) in TMTA139 strains was approximately 26.5-fold higher than that of the parental strain RUT C30 at 72 h of cultivation in a shake flask using glucose as the sole carbon source. Furthermore, the crude enzymes produced in the 7-L fermenter from TMTA66 and TMTA139 supplemented with commercial β-glucosidase hydrolyzed pretreated corn stover effectively. CONCLUSIONS: These results show that replacing natural transcription factors with minimal transcriptional activators is a powerful strategy to enhance cellulase production in T. reesei. Our current study also offers an alternative genetic engineering strategy for the enhanced production of industrial products by other fungi. SN - 1475-2859 UR - https://www.unboundmedicine.com/medline/citation/29773074/Enhanced_cellulase_production_in_Trichoderma_reesei_RUT_C30_via_constitution_of_minimal_transcriptional_activators_ L2 - https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-018-0926-7 DB - PRIME DP - Unbound Medicine ER -