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The effect of syndecan-4 and glypican-1 knockdown on the proliferation and differentiation of turkey satellite cells differing in age and growth rates.

Abstract

Posthatch skeletal muscle growth requires myogenic satellite cells and the dynamic expression of cell membrane-associated proteins. The membrane associated heparan sulfate proteoglycans, syndecan-4 and glypican-1, link the satellite cell niche to the intracellular environment. Sydnecan-4 and glypican-1 are differentially expressed with age in turkey satellite cells and their over-expression impacts both satellite cell proliferation and differentiation, but their effect on satellite cells from lines with different growth potentials is not known. The objective of the current study was to determine if syndecan-4 and glypican-1 regulation of satellite cell proliferation and differentiation is affected by age and growth selection. Pectoralis major satellite cells isolated at 1 d, 7 and 16-wk of age from a Randombred Control 2 (RBC2) line and a 16-wk body weight (F) line selected from the RBC2 line turkeys were studied. Syndecan-4 and glypican-1 expression was knocked down in both lines. The F-line cells proliferated faster than RBC2 line cells regardless of age, while differentiation tended to be greater in RBC2 line cells than F-line cells at each age. Syndecan-4 knockdown decreased proliferation at 7- and 16-wk but not 1 d cells, and increased differentiation at 1 d and 7 wk but not 16 wk cells. Glypican-1 knockdown differentially affected proliferation depending on cell age, whereas differentiation was decreased for 7- and 16-wk but not 1 d cells. These data suggest syndecan-4 and glypican-1 differentially affected satellite cell function in an age-dependent manner, but had little impact on differences in proliferation and differentiation due to growth selection.

Authors+Show Affiliations

Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States. Electronic address: Velleman.1@osu.edu.Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States.Department of Animal Sciences, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, United States.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29775666

Citation

Velleman, Sandra G., et al. "The Effect of Syndecan-4 and Glypican-1 Knockdown On the Proliferation and Differentiation of Turkey Satellite Cells Differing in Age and Growth Rates." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 223, 2018, pp. 33-41.
Velleman SG, Clark DL, Tonniges JR. The effect of syndecan-4 and glypican-1 knockdown on the proliferation and differentiation of turkey satellite cells differing in age and growth rates. Comp Biochem Physiol A Mol Integr Physiol. 2018;223:33-41.
Velleman, S. G., Clark, D. L., & Tonniges, J. R. (2018). The effect of syndecan-4 and glypican-1 knockdown on the proliferation and differentiation of turkey satellite cells differing in age and growth rates. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 223, 33-41. https://doi.org/10.1016/j.cbpa.2018.05.014
Velleman SG, Clark DL, Tonniges JR. The Effect of Syndecan-4 and Glypican-1 Knockdown On the Proliferation and Differentiation of Turkey Satellite Cells Differing in Age and Growth Rates. Comp Biochem Physiol A Mol Integr Physiol. 2018;223:33-41. PubMed PMID: 29775666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effect of syndecan-4 and glypican-1 knockdown on the proliferation and differentiation of turkey satellite cells differing in age and growth rates. AU - Velleman,Sandra G, AU - Clark,Daniel L, AU - Tonniges,Jeffrey R, Y1 - 2018/05/19/ PY - 2018/02/14/received PY - 2018/04/19/revised PY - 2018/05/08/accepted PY - 2018/5/19/pubmed PY - 2019/3/16/medline PY - 2018/5/19/entrez KW - Glypican-1 KW - Muscle KW - Satellite cell KW - Syndecan-4 KW - Turkey SP - 33 EP - 41 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 223 N2 - Posthatch skeletal muscle growth requires myogenic satellite cells and the dynamic expression of cell membrane-associated proteins. The membrane associated heparan sulfate proteoglycans, syndecan-4 and glypican-1, link the satellite cell niche to the intracellular environment. Sydnecan-4 and glypican-1 are differentially expressed with age in turkey satellite cells and their over-expression impacts both satellite cell proliferation and differentiation, but their effect on satellite cells from lines with different growth potentials is not known. The objective of the current study was to determine if syndecan-4 and glypican-1 regulation of satellite cell proliferation and differentiation is affected by age and growth selection. Pectoralis major satellite cells isolated at 1 d, 7 and 16-wk of age from a Randombred Control 2 (RBC2) line and a 16-wk body weight (F) line selected from the RBC2 line turkeys were studied. Syndecan-4 and glypican-1 expression was knocked down in both lines. The F-line cells proliferated faster than RBC2 line cells regardless of age, while differentiation tended to be greater in RBC2 line cells than F-line cells at each age. Syndecan-4 knockdown decreased proliferation at 7- and 16-wk but not 1 d cells, and increased differentiation at 1 d and 7 wk but not 16 wk cells. Glypican-1 knockdown differentially affected proliferation depending on cell age, whereas differentiation was decreased for 7- and 16-wk but not 1 d cells. These data suggest syndecan-4 and glypican-1 differentially affected satellite cell function in an age-dependent manner, but had little impact on differences in proliferation and differentiation due to growth selection. SN - 1531-4332 UR - https://www.unboundmedicine.com/medline/citation/29775666/The_effect_of_syndecan_4_and_glypican_1_knockdown_on_the_proliferation_and_differentiation_of_turkey_satellite_cells_differing_in_age_and_growth_rates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095-6433(18)30070-9 DB - PRIME DP - Unbound Medicine ER -