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Chlorophyll a fluorescence and transcriptome reveal the toxicological effects of bisphenol A on an invasive cyanobacterium, Cylindrospermopsis raciborskii.
Aquat Toxicol. 2018 Jul; 200:188-196.AT

Abstract

Bisphenol A has attracted worldwide attention due to its harmful effects on humans, animals and plants. In this study, the toxicological effects of BPA on Cylindrospermopsis raciborskii were assessed based on chlorophyll a fluorescence and transcriptome analyses. The results showed that the growth of C. raciborskii was significantly inhibited when BPA exceeded 0.1 mg L-1. A marked rise of phase J was observed at a concentration greater than 0.1 mg L-1, while a K phase appeared at 20 mg L-1. The chlorophyll a fluorescence parameters of RC/CS0, F0, φP0, φE0, and ψ0, underwent a significant decline under all treatments of BPA, whereas a significant increase in both VJ and M0 occurred under all concentrations of BPA. Additionally, ABS/RC and DIo/RC markedly increased at 10 mg L-1 and 20 mg L-1. The transcriptome analysis revealed that the genes of photosynthesis, including psbA, psbB, psbC, psbD, apcA, apcB, cpcA, and cpcB, as well as those of chlorophyll and carotenoid biosynthesis, namely hemN, acsF, chlL, chlN, chlP, crtB, pds, were all down-regulated. Moreover, BPA also inhibited the oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), and fatty acid metabolism in C. raciborskii. Taken together, these results suggest BPA can negatively affect the expression of multiple genes and the vital energy metabolism process to arrest the growth and photosynthesis of C. raciborskii.

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Authors+Show Affiliations

Xiang R
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Shi J
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Zhang H
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Dong C
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Liu L
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Fu J
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
He X
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Yan Y
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China.
Wu Z
Key Laboratory of Eco-environments in Three Gorges Reservoir Region (Ministry of Education), Chongqing Key Laboratory of Plant Ecology and Resources Research in Three Gorges Reservoir Region, School of Life Science, Southwest University, Chongqing, 400715, PR China. Electronic address: wuzhx@swu.edu.cn.

MeSH

Benzhydryl CompoundsChlorophyllChlorophyll ACylindrospermopsisDown-RegulationFluorescenceGene Expression ProfilingMetabolic Networks and PathwaysPhenolsPhotosynthesisTranscriptomeWater Pollutants, Chemical

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29775926

Citation

Xiang, Rong, et al. "Chlorophyll a FLuorescence and Transcriptome Reveal the Toxicological Effects of Bisphenol a On an Invasive Cyanobacterium, Cylindrospermopsis Raciborskii." Aquatic Toxicology (Amsterdam, Netherlands), vol. 200, 2018, pp. 188-196.
Xiang R, Shi J, Zhang H, et al. Chlorophyll a fluorescence and transcriptome reveal the toxicological effects of bisphenol A on an invasive cyanobacterium, Cylindrospermopsis raciborskii. Aquat Toxicol. 2018;200:188-196.
Xiang, R., Shi, J., Zhang, H., Dong, C., Liu, L., Fu, J., He, X., Yan, Y., & Wu, Z. (2018). Chlorophyll a fluorescence and transcriptome reveal the toxicological effects of bisphenol A on an invasive cyanobacterium, Cylindrospermopsis raciborskii. Aquatic Toxicology (Amsterdam, Netherlands), 200, 188-196. https://doi.org/10.1016/j.aquatox.2018.05.005
Xiang R, et al. Chlorophyll a FLuorescence and Transcriptome Reveal the Toxicological Effects of Bisphenol a On an Invasive Cyanobacterium, Cylindrospermopsis Raciborskii. Aquat Toxicol. 2018;200:188-196. PubMed PMID: 29775926.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chlorophyll a fluorescence and transcriptome reveal the toxicological effects of bisphenol A on an invasive cyanobacterium, Cylindrospermopsis raciborskii. AU - Xiang,Rong, AU - Shi,Junqiong, AU - Zhang,Hongbo, AU - Dong,Congcong, AU - Liu,Li, AU - Fu,JunKe, AU - He,Xinyu, AU - Yan,Yanjun, AU - Wu,Zhongxing, Y1 - 2018/05/09/ PY - 2018/01/29/received PY - 2018/05/07/revised PY - 2018/05/07/accepted PY - 2018/5/19/pubmed PY - 2018/7/26/medline PY - 2018/5/19/entrez KW - Bisphenol A KW - Chlorophyll a fluorescence KW - Cylindrospermopsis raciborskii KW - PS II KW - Transcriptome SP - 188 EP - 196 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 200 N2 - Bisphenol A has attracted worldwide attention due to its harmful effects on humans, animals and plants. In this study, the toxicological effects of BPA on Cylindrospermopsis raciborskii were assessed based on chlorophyll a fluorescence and transcriptome analyses. The results showed that the growth of C. raciborskii was significantly inhibited when BPA exceeded 0.1 mg L-1. A marked rise of phase J was observed at a concentration greater than 0.1 mg L-1, while a K phase appeared at 20 mg L-1. The chlorophyll a fluorescence parameters of RC/CS0, F0, φP0, φE0, and ψ0, underwent a significant decline under all treatments of BPA, whereas a significant increase in both VJ and M0 occurred under all concentrations of BPA. Additionally, ABS/RC and DIo/RC markedly increased at 10 mg L-1 and 20 mg L-1. The transcriptome analysis revealed that the genes of photosynthesis, including psbA, psbB, psbC, psbD, apcA, apcB, cpcA, and cpcB, as well as those of chlorophyll and carotenoid biosynthesis, namely hemN, acsF, chlL, chlN, chlP, crtB, pds, were all down-regulated. Moreover, BPA also inhibited the oxidative phosphorylation, glycolysis/gluconeogenesis, citrate cycle (TCA cycle), and fatty acid metabolism in C. raciborskii. Taken together, these results suggest BPA can negatively affect the expression of multiple genes and the vital energy metabolism process to arrest the growth and photosynthesis of C. raciborskii. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/29775926/Chlorophyll_a_fluorescence_and_transcriptome_reveal_the_toxicological_effects_of_bisphenol_A_on_an_invasive_cyanobacterium_Cylindrospermopsis_raciborskii_ DB - PRIME DP - Unbound Medicine ER -