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Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens.
Viruses. 2018 05 18; 10(5)V

Abstract

Superinfection of Marek's disease virus (MDV) and avian leukosis virus subgroup J (ALV-J) causes lethal neoplasia and death in chickens. However, whether there is synergism between the two viruses in viral replication and pathogenicity has remained elusive. In this study, we found that the superinfection of MDV and ALV-J increased the viral replication of the two viruses in RNA and protein level, and synergistically promoted the expression of IL-10, IL-6, and TGF-β in chicken embryo fibroblasts (CEF). Moreover, MDV and ALV-J protein expression in dual-infected cells detected by confocal laser scanning microscope appeared earlier in the cytoplasm and the nucleus, and caused more severe cytopathy than single infection, suggesting that synergistically increased MDV and ALV-J viral-protein biosynthesis is responsible for the severe cytopathy. In vivo, compared to the single virus infected chickens, the mortality and tumor formation rates increased significantly in MDV and ALV-J dual-infected chickens. Viral loads of MDV and ALV-J in tissues of dual-infected chickens were significantly higher than those of single-infected chickens. Histopathology observation showed that more severe inflammation and tumor cells metastases were present in dual-infected chickens. In the present study, we concluded that synergistic viral replication of MDV and ALV-J is responsible for the enhanced pathogenicity in superinfection of chickens.

Authors+Show Affiliations

College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. tiramisurd@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. 13515381026@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. m15253197976@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. duxusheng12345@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. ssu6307@163.com.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. chgli1981@126.com. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China. chgli1981@126.com.The Pirbright Institute & UK-China Centre of Excellence on Avian Disease Research, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UK. venugopal.nair@pirbright.ac.uk.The Pirbright Institute & UK-China Centre of Excellence on Avian Disease Research, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UK. yongxiu.yao@pirbright.ac.uk.College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China. honey@sdau.edu.cn. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an 271018, China. honey@sdau.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29783672

Citation

Zhou, Jing, et al. "Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J Is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens." Viruses, vol. 10, no. 5, 2018.
Zhou J, Zhao GL, Wang XM, et al. Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens. Viruses. 2018;10(5).
Zhou, J., Zhao, G. L., Wang, X. M., Du, X. S., Su, S., Li, C. G., Nair, V., Yao, Y. X., & Cheng, Z. Q. (2018). Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens. Viruses, 10(5). https://doi.org/10.3390/v10050271
Zhou J, et al. Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J Is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens. Viruses. 2018 05 18;10(5) PubMed PMID: 29783672.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Synergistic Viral Replication of Marek's Disease Virus and Avian Leukosis Virus Subgroup J is Responsible for the Enhanced Pathogenicity in the Superinfection of Chickens. AU - Zhou,Jing, AU - Zhao,Guo-Liang, AU - Wang,Xiao-Man, AU - Du,Xu-Sheng, AU - Su,Shuai, AU - Li,Chen-Gui, AU - Nair,Venugopal, AU - Yao,Yong-Xiu, AU - Cheng,Zi-Qiang, Y1 - 2018/05/18/ PY - 2018/03/31/received PY - 2018/05/09/revised PY - 2018/05/15/accepted PY - 2018/5/23/entrez PY - 2018/5/23/pubmed PY - 2018/11/28/medline KW - Marek’s diseases virus KW - avian leukosis virus subgroup J KW - pathogenicity KW - superinfection KW - synergism JF - Viruses JO - Viruses VL - 10 IS - 5 N2 - Superinfection of Marek's disease virus (MDV) and avian leukosis virus subgroup J (ALV-J) causes lethal neoplasia and death in chickens. However, whether there is synergism between the two viruses in viral replication and pathogenicity has remained elusive. In this study, we found that the superinfection of MDV and ALV-J increased the viral replication of the two viruses in RNA and protein level, and synergistically promoted the expression of IL-10, IL-6, and TGF-β in chicken embryo fibroblasts (CEF). Moreover, MDV and ALV-J protein expression in dual-infected cells detected by confocal laser scanning microscope appeared earlier in the cytoplasm and the nucleus, and caused more severe cytopathy than single infection, suggesting that synergistically increased MDV and ALV-J viral-protein biosynthesis is responsible for the severe cytopathy. In vivo, compared to the single virus infected chickens, the mortality and tumor formation rates increased significantly in MDV and ALV-J dual-infected chickens. Viral loads of MDV and ALV-J in tissues of dual-infected chickens were significantly higher than those of single-infected chickens. Histopathology observation showed that more severe inflammation and tumor cells metastases were present in dual-infected chickens. In the present study, we concluded that synergistic viral replication of MDV and ALV-J is responsible for the enhanced pathogenicity in superinfection of chickens. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/29783672/Synergistic_Viral_Replication_of_Marek's_Disease_Virus_and_Avian_Leukosis_Virus_Subgroup_J_is_Responsible_for_the_Enhanced_Pathogenicity_in_the_Superinfection_of_Chickens_ L2 - http://www.mdpi.com/resolver?pii=v10050271 DB - PRIME DP - Unbound Medicine ER -