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4-Mercaptophenylboronic acid-modified spirally-curved mesoporous silica nanofibers coupled with ultra performance liquid chromatography-mass spectrometry for determination of brassinosteroids in plants.
Food Chem. 2018 Oct 15; 263:51-58.FC

Abstract

Herein, for the first time, thiol-functionalized mesoporous silica (mSiO2-SH) nanofibers with a spirally-curved twisted hexagonal morphology were synthesized via a simple one-pot protocol. 4-Mercaptophenylboronic acids (4-MPBA) were attached onto the mSiO2-SH nanofibers via disulfide bond, serving as boronate affinity sorbent to selectively capture brassinosteroids (BRs) from plant extract. The resulting BRs-MPBA derivatives were easily eluted from the sorbent by cleaving the disulfide bond, which was subsequently subjected to ultra performance liquid chromatography-mass spectrometry (UPLC-MS) analysis. Thus, the in situ extraction/derivatization/desorption method coupled with UPLC-MS was established for the fast, sensitive and selective detection of BRs in plant tissues. Finally, based on the developed method, endogenous BRs were successfully detected in leaf of H. lupulus L., silique of A. thaliana, and panicle of O. sativa L.

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Publisher Full Text (DOI)

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.

Institute of Chinese Medical Sciences, State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Taipa, Macau. Electronic address: jianboxiao@yahoo.com.

College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; Hunan Provincial Key Laboratory of Efficient and Clean Utilization of Manganese Resources, Central South University, Changsha 410083, Hunan, China. Electronic address: xqchen@csu.edu.cn.

MeSH

ArabidopsisBoronic AcidsBrassinosteroidsChromatography, LiquidHumulusMass SpectrometryNanofibersOryzaPlant ExtractsPlant LeavesPlantsSilicon DioxideSulfhydryl Compounds

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29784327

Citation

Chen, Miao, et al. "4-Mercaptophenylboronic Acid-modified Spirally-curved Mesoporous Silica Nanofibers Coupled With Ultra Performance Liquid Chromatography-mass Spectrometry for Determination of Brassinosteroids in Plants." Food Chemistry, vol. 263, 2018, pp. 51-58.

Chen M, Wang R, Zhu Y, et al. 4-Mercaptophenylboronic acid-modified spirally-curved mesoporous silica nanofibers coupled with ultra performance liquid chromatography-mass spectrometry for determination of brassinosteroids in plants. Food Chem. 2018;263:51-58.

Chen, M., Wang, R., Zhu, Y., Liu, M., Zhu, F., Xiao, J., & Chen, X. (2018). 4-Mercaptophenylboronic acid-modified spirally-curved mesoporous silica nanofibers coupled with ultra performance liquid chromatography-mass spectrometry for determination of brassinosteroids in plants. Food Chemistry, 263, 51-58. https://doi.org/10.1016/j.foodchem.2018.04.129

Chen M, et al. 4-Mercaptophenylboronic Acid-modified Spirally-curved Mesoporous Silica Nanofibers Coupled With Ultra Performance Liquid Chromatography-mass Spectrometry for Determination of Brassinosteroids in Plants. Food Chem. 2018 Oct 15;263:51-58. PubMed PMID: 29784327.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - 4-Mercaptophenylboronic acid-modified spirally-curved mesoporous silica nanofibers coupled with ultra performance liquid chromatography-mass spectrometry for determination of brassinosteroids in plants. AU - Chen,Miao, AU - Wang,Ranhao, AU - Zhu,Yuqiu, AU - Liu,Minzhuo, AU - Zhu,Fawei, AU - Xiao,Jianbo, AU - Chen,Xiaoqing, Y1 - 2018/04/30/ PY - 2018/02/09/received PY - 2018/04/26/revised PY - 2018/04/28/accepted PY - 2018/5/23/entrez PY - 2018/5/23/pubmed PY - 2018/12/12/medline KW - Endogenous brassinosteroids KW - In situ extraction/derivatization/desorption KW - Mesoporous silica nanofibers KW - Ultra performance liquid chromatography–mass spectrometry SP - 51 EP - 58 JF - Food chemistry JO - Food Chem VL - 263 N2 - Herein, for the first time, thiol-functionalized mesoporous silica (mSiO2-SH) nanofibers with a spirally-curved twisted hexagonal morphology were synthesized via a simple one-pot protocol. 4-Mercaptophenylboronic acids (4-MPBA) were attached onto the mSiO2-SH nanofibers via disulfide bond, serving as boronate affinity sorbent to selectively capture brassinosteroids (BRs) from plant extract. The resulting BRs-MPBA derivatives were easily eluted from the sorbent by cleaving the disulfide bond, which was subsequently subjected to ultra performance liquid chromatography-mass spectrometry (UPLC-MS) analysis. Thus, the in situ extraction/derivatization/desorption method coupled with UPLC-MS was established for the fast, sensitive and selective detection of BRs in plant tissues. Finally, based on the developed method, endogenous BRs were successfully detected in leaf of H. lupulus L., silique of A. thaliana, and panicle of O. sativa L. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/29784327/4_Mercaptophenylboronic_acid_modified_spirally_curved_mesoporous_silica_nanofibers_coupled_with_ultra_performance_liquid_chromatography_mass_spectrometry_for_determination_of_brassinosteroids_in_plants_ DB - PRIME DP - Unbound Medicine ER -