Tags

Type your tag names separated by a space and hit enter

[EFFECT OF RECOMBINANT CO-EXPRESSION ADENOVIRUS OF NERVE GROWTH FACTOR AND MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY].

Abstract

OBJECTIVE

To construct recombinant adenovirus expressing nerve growth factor (NGF) and myelin associated glycoprotein (MAG) (Ad-NGF-MAG) and to investigate its effect on repair and regeneration of sciatic nerve injury in rats.

METHODS

NGF and MAG gene sequences were cloned into shuttle plasmid pCA13 of adenovirus type 5. After packed in HEK293 cells, the recombinant Ad-NGF-MAG underwent sequence and identification. Thirty-two male Sprague Dawley rats were randomly divided into 4 groups (n=8): control group (normal control), adenovirus vector group (Ad group), Ad-NGF group, and Ad-NGF-MAG group. The sciatic nerve injury model was established by transection of the right sciatic nerve; then, the empty adenovirus vector, Ad-NGF, and Ad-NGF-MAG were injected into the gastrocnemius muscle of the affected limb at a dose of 1×108 PFU every other day for 3 times in Ad group, AdNGF group, and Ad-NGF-MAG group, respectively. The right sciatic nerve was exposed only, and then the incision was closed in the control group. The sciatic nerve function index (SFI) was measured, and neuro-electrophysiology was observed; mRNA and protein expressions of NGF and MAG were detected by RT-PCR and Western blot; and histological examination was performed at 31 days after operation.

RESULTS

Recombinant adenovirus vectors of Ad-NGF and Ad-NGF-MAG were constructed successfully. All rats survived and incision healed by first intension. The SFI, nerve conduction velocity, evoked potential amplitude, and latent period of Ad-NGF-MAG group were significantly better than those of Ad group and Ad-NGF group (P<0.05). MAG mRNA and protein expressions of Ad-NGF-MAG group were the highest in all the groups (P<0.05). The expressions of NGF mRNA and protein increased in Ad-NGF group and AdNGF-MAG group when compared with control group and Ad group (P<0.05). Histological examination showed that the nerve had good continuity in control group; nerve fibers disarranged in Ad group; neurons connections formed in some nerve fibers of Ad-NGF group, but nerve fibers arrange disorderly; and the growth of the nerve were ordered and wellstructured in Ad-NGF-MAG group.

CONCLUSIONS

Ad-NGF-MAG can effectively promote the growth of the nerve and inhibit the form of abnormal branches, facilitating the repair of sciatic nerve injury in rats.

Authors+Show Affiliations

The Fisrt Department of Orthopedics, Chongqing Ninth People's Hospital, Chongqing, 400070, P. R. China.Department of Orthopedics, the Second Affiliated Hospital of Chongqing Medical University.The Fisrt Department of Orthopedics, Chongqing Ninth People's Hospital, Chongqing, 400070, P. R. China.The Fisrt Department of Orthopedics, Chongqing Ninth People's Hospital, Chongqing, 400070, P. R. China.The Fisrt Department of Orthopedics, Chongqing Ninth People's Hospital, Chongqing, 400070, P. R. China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29786236

Citation

Chen, Yu, et al. "[EFFECT of RECOMBINANT CO-EXPRESSION ADENOVIRUS of NERVE GROWTH FACTOR and MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY]." Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, vol. 30, no. 8, 2016, pp. 1026-1033.
Chen Y, Deng Z, Weng Z, et al. [EFFECT OF RECOMBINANT CO-EXPRESSION ADENOVIRUS OF NERVE GROWTH FACTOR AND MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2016;30(8):1026-1033.
Chen, Y., Deng, Z., Weng, Z., Chen, S., & Tong, W. (2016). [EFFECT OF RECOMBINANT CO-EXPRESSION ADENOVIRUS OF NERVE GROWTH FACTOR AND MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi = Zhongguo Xiufu Chongjian Waike Zazhi = Chinese Journal of Reparative and Reconstructive Surgery, 30(8), pp. 1026-1033. doi:10.7507/1002-1892.20160206.
Chen Y, et al. [EFFECT of RECOMBINANT CO-EXPRESSION ADENOVIRUS of NERVE GROWTH FACTOR and MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY]. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2016 Aug 8;30(8):1026-1033. PubMed PMID: 29786236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [EFFECT OF RECOMBINANT CO-EXPRESSION ADENOVIRUS OF NERVE GROWTH FACTOR AND MYELIN ASSOCIATED GLYCOPROTEIN GENES ON RAT SCIATIC NERVE INJURY]. AU - Chen,Yu, AU - Deng,Zhongliang, AU - Weng,Zheng, AU - Chen,Shimou, AU - Tong,Wei, PY - 2018/5/23/entrez PY - 2016/8/8/pubmed PY - 2016/8/8/medline KW - Adenovirus KW - Gene therapy KW - Myelin associated glycoprotein KW - Nerve growth factor KW - Nerve repair KW - Rat KW - Sciatic nerve injury SP - 1026 EP - 1033 JF - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery JO - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi VL - 30 IS - 8 N2 - OBJECTIVE: To construct recombinant adenovirus expressing nerve growth factor (NGF) and myelin associated glycoprotein (MAG) (Ad-NGF-MAG) and to investigate its effect on repair and regeneration of sciatic nerve injury in rats. METHODS: NGF and MAG gene sequences were cloned into shuttle plasmid pCA13 of adenovirus type 5. After packed in HEK293 cells, the recombinant Ad-NGF-MAG underwent sequence and identification. Thirty-two male Sprague Dawley rats were randomly divided into 4 groups (n=8): control group (normal control), adenovirus vector group (Ad group), Ad-NGF group, and Ad-NGF-MAG group. The sciatic nerve injury model was established by transection of the right sciatic nerve; then, the empty adenovirus vector, Ad-NGF, and Ad-NGF-MAG were injected into the gastrocnemius muscle of the affected limb at a dose of 1×108 PFU every other day for 3 times in Ad group, AdNGF group, and Ad-NGF-MAG group, respectively. The right sciatic nerve was exposed only, and then the incision was closed in the control group. The sciatic nerve function index (SFI) was measured, and neuro-electrophysiology was observed; mRNA and protein expressions of NGF and MAG were detected by RT-PCR and Western blot; and histological examination was performed at 31 days after operation. RESULTS: Recombinant adenovirus vectors of Ad-NGF and Ad-NGF-MAG were constructed successfully. All rats survived and incision healed by first intension. The SFI, nerve conduction velocity, evoked potential amplitude, and latent period of Ad-NGF-MAG group were significantly better than those of Ad group and Ad-NGF group (P<0.05). MAG mRNA and protein expressions of Ad-NGF-MAG group were the highest in all the groups (P<0.05). The expressions of NGF mRNA and protein increased in Ad-NGF group and AdNGF-MAG group when compared with control group and Ad group (P<0.05). Histological examination showed that the nerve had good continuity in control group; nerve fibers disarranged in Ad group; neurons connections formed in some nerve fibers of Ad-NGF group, but nerve fibers arrange disorderly; and the growth of the nerve were ordered and wellstructured in Ad-NGF-MAG group. CONCLUSIONS: Ad-NGF-MAG can effectively promote the growth of the nerve and inhibit the form of abnormal branches, facilitating the repair of sciatic nerve injury in rats. SN - 1002-1892 UR - https://www.unboundmedicine.com/medline/citation/29786236/[EFFECT_OF_RECOMBINANT_CO_EXPRESSION_ADENOVIRUS_OF_NERVE_GROWTH_FACTOR_AND_MYELIN_ASSOCIATED_GLYCOPROTEIN_GENES_ON_RAT_SCIATIC_NERVE_INJURY]_ DB - PRIME DP - Unbound Medicine ER -