TY - JOUR T1 - Chemical study, antioxidant, anti-hypertensive, and cytotoxic/cytoprotective activities of Centaurea cyanus L. petals aqueous extract. AU - Escher,Graziela Bragueto, AU - Santos,Jânio Sousa, AU - Rosso,Neiva Deliberali, AU - Marques,Mariza Boscacci, AU - Azevedo,Luciana, AU - do Carmo,Mariana Araújo Vieira, AU - Daguer,Heitor, AU - Molognoni,Luciano, AU - Prado-Silva,Leonardo do, AU - Sant'Ana,Anderson S, AU - da Silva,Marcia Cristina, AU - Granato,Daniel, Y1 - 2018/05/19/ PY - 2018/04/07/received PY - 2018/05/16/revised PY - 2018/05/18/accepted PY - 2018/5/23/pubmed PY - 2018/10/26/medline PY - 2018/5/23/entrez KW - Desirability function KW - Flavonoids KW - LC-ESI-MS/MS KW - Phenolic compounds KW - Protocyanins KW - Response surface methodology SP - 439 EP - 453 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 118 N2 - This study aimed to optimise the experimental conditions of extraction of the phytochemical compounds and functional properties of Centaurea cyanus petals. The following parameters were determined: the chemical composition (LC-ESI-MS/MS), the effects of pH on the stability and antioxidant activity of anthocyanins, the inhibition of lipid peroxidation, antioxidant activity, anti-hemolytic activity, antimicrobial, anti-hypertensive, and cytotoxic/cytoprotective effect, and the measurements of intracellular reactive oxygen species. Results showed that the temperature and time influenced (p ≤ 0.05) the content of flavonoids, anthocyanins, and FRAP. Only the temperature influenced the total phenolic content, non-anthocyanin flavonoids, and antioxidant activity (DPPH). The statistical approach made it possible to obtain the optimised experimental extraction conditions to increase the level of bioactive compounds. Chlorogenic, caffeic, ferulic, and p-coumaric acids, isoquercitrin, and coumarin were identified as the major compounds in the optimised extract. The optimised extract presented anti-hemolytic and anti-hypertensive activity in vitro, in addition to showing stability and reversibility of anthocyanins and antioxidant activity with pH variation. The C. cyanus petals aqueous extract exhibited high IC50 and GI50 (>900 μg/mL) values for all cell lines, meaning low cytotoxicity. Based on the stress oxidative assay, the extract exhibited pro-oxidant action (10-100 μg/mL) but did not cause damage or cell death. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/29787846/Chemical_study_antioxidant_anti_hypertensive_and_cytotoxic/cytoprotective_activities_of_Centaurea_cyanus_L__petals_aqueous_extract_ DB - PRIME DP - Unbound Medicine ER -