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Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees.
Braz J Med Biol Res 2018; 51(6):e7118BJ

Abstract

In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed that the EEP of M. quadrifasciata quadrifasciata has a more complex chemical composition than the EEP of T. angustula. Moreover, UPLC-MS analyses of M. quadrifasciata quadrifascita indicated flavonoids and terpenes as major constituents. The bactericidal activity of both EEPs was higher against gram-positive bacteria than for gram-negative bacteria. The EEP from M. quadrifasciata quadrifasciata presented MIC values lower than the EEP from T. angustula for all tested bacteria. The EEP from M. quadrifasciata quadrifasciata caused lysis of the bacterial wall and release of intracellular components from both E. coli and S. aureus. Our findings indicate that the chemical composition of propolis from stingless bees is complex and depends on the species. The extract from M. quadrifasciata quadrifascita was more effective against gram-positive than gram-negative strains, especially against S. aureus and methicillin-resistant S. aureus compared to T. angustula extract, by a mechanism that involves disturbance of the bacterial cell membrane integrity.

Authors+Show Affiliations

Programa de Pós-graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.Laboratorio de Neurobiologia da Dor e Inflamação, Departamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.Laboratório de Morfogênese e Bioquímica Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.Laboratório de Morfogênese e Bioquímica Vegetal, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, Florianópolis, SC, Brasil.Programa de Pós-graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.Programa de Pós-graduação em Farmacologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29791598

Citation

Torres, A R., et al. "Chemical Characterization, Antioxidant and Antimicrobial Activity of Propolis Obtained From Melipona Quadrifasciata Quadrifasciata and Tetragonisca Angustula Stingless Bees." Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, vol. 51, no. 6, 2018, pp. e7118.
Torres AR, Sandjo LP, Friedemann MT, et al. Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Braz J Med Biol Res. 2018;51(6):e7118.
Torres, A. R., Sandjo, L. P., Friedemann, M. T., Tomazzoli, M. M., Maraschin, M., Mello, C. F., & Santos, A. R. S. (2018). Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Medicas E Biologicas, 51(6), pp. e7118. doi:10.1590/1414-431x20187118.
Torres AR, et al. Chemical Characterization, Antioxidant and Antimicrobial Activity of Propolis Obtained From Melipona Quadrifasciata Quadrifasciata and Tetragonisca Angustula Stingless Bees. Braz J Med Biol Res. 2018;51(6):e7118. PubMed PMID: 29791598.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chemical characterization, antioxidant and antimicrobial activity of propolis obtained from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula stingless bees. AU - Torres,A R, AU - Sandjo,L P, AU - Friedemann,M T, AU - Tomazzoli,M M, AU - Maraschin,M, AU - Mello,C F, AU - Santos,A R S, Y1 - 2018/05/21/ PY - 2017/09/12/received PY - 2018/02/16/accepted PY - 2018/5/24/entrez PY - 2018/5/24/pubmed PY - 2018/7/11/medline SP - e7118 EP - e7118 JF - Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas JO - Braz. J. Med. Biol. Res. VL - 51 IS - 6 N2 - In this study, we investigated the chemical composition, and antioxidant and antibacterial properties of ethanolic extracts of propolis (EEP) from Melipona quadrifasciata quadrifasciata and Tetragonisca angustula. Chemical composition of EEP was determined by colorimetry and chromatographic (HPLC-DAD and UPLC-Q/TOF-MS/MS) analysis. Antimicrobial activity of EEP was evaluated against gram-positive (S. aureus, methicillin-resistant S. aureus, E. faecalis) and gram-negative (E. coli and K. pneumoniae) bacteria by the minimal inhibitory concentration (MIC) test using the microdilution method. Furthermore, the growth curve and integrity of cell membrane of S. aureus and E. coli were investigated using standard microbiological methods. HPLC-DAD analysis showed that the EEP of M. quadrifasciata quadrifasciata has a more complex chemical composition than the EEP of T. angustula. Moreover, UPLC-MS analyses of M. quadrifasciata quadrifascita indicated flavonoids and terpenes as major constituents. The bactericidal activity of both EEPs was higher against gram-positive bacteria than for gram-negative bacteria. The EEP from M. quadrifasciata quadrifasciata presented MIC values lower than the EEP from T. angustula for all tested bacteria. The EEP from M. quadrifasciata quadrifasciata caused lysis of the bacterial wall and release of intracellular components from both E. coli and S. aureus. Our findings indicate that the chemical composition of propolis from stingless bees is complex and depends on the species. The extract from M. quadrifasciata quadrifascita was more effective against gram-positive than gram-negative strains, especially against S. aureus and methicillin-resistant S. aureus compared to T. angustula extract, by a mechanism that involves disturbance of the bacterial cell membrane integrity. SN - 1414-431X UR - https://www.unboundmedicine.com/medline/citation/29791598/Chemical_characterization_antioxidant_and_antimicrobial_activity_of_propolis_obtained_from_Melipona_quadrifasciata_quadrifasciata_and_Tetragonisca_angustula_stingless_bees_ L2 - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000600617&lng=en&nrm=iso&tlng=en DB - PRIME DP - Unbound Medicine ER -