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Relationship between modification of activated sludge wastewater treatment and changes in antibiotic resistance of bacteria.
Sci Total Environ. 2018 Oct 15; 639:304-315.ST

Abstract

Biological treatment processes at wastewater treatment plants (WWTPs), which are the most common methods of sewage treatment, could cause selective elimination and/or changes in the proportions of phenotypes/genotypes within bacterial populations in effluent. Therefore, WWTPs based on activated sludge used in sewage treatment constitute an important reservoir of enteric bacteria which harbour potentially transferable resistance genes. Together with treated wastewater, these microorganisms can penetrate the soil, surface water, rural groundwater supplies and drinking water. Because of this, the aim of this study was to determine the impact of various modification of sewage treatment (the conventional anaerobic/anoxic/oxic (A2/O) process, mechanical-biological (MB) system, sequencing batch reactors (SBR), mechanical-biological system with elevated removal of nutrients (MB-ERN)) on the amount of antibiotic resistant bacteria (ARB) (including E. coli) and antibiotic resistance genes (ARGs) in sewage flowing out of the 13 treatment plants using activated sludge technology. There were no significant differences in ARB and ARGs regardless of time of sampling and type of treated wastewater (p > 0.05). The highest percentage of reduction (up to 99.9%) in the amount of ARB and ARGs was observed in WWTPs with MB and MB-ERN systems. The lowest reduction was detected in WWTPs with SBR. A significant increase (p < 0.05) in the percentage of bacteria resistant to the new generation antibiotics (CTX and DOX) in total counts of microorganisms was observed in effluents (EFF) from WWTPs with A2/O system and with SBR. Among all ARGs analyzed, the highest prevalence of ARGs copies in EFF samples was observed for sul1, tet(A) and qepA, the lowest for blaTEM and blaSHV. Although, the results of presented study demonstrate high efficiency of ARB and ARGs removal during the wastewater treatment processes, especially by WWTPs with MB and MB-ERN systems, EFF is still an important reservoir of ARGs which can be transferred to other microorganisms.

Authors+Show Affiliations

Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: ewa.korzeniewska@uwm.edu.pl.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: monika.harnisz@uwm.edu.pl.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29791883

Citation

Korzeniewska, Ewa, and Monika Harnisz. "Relationship Between Modification of Activated Sludge Wastewater Treatment and Changes in Antibiotic Resistance of Bacteria." The Science of the Total Environment, vol. 639, 2018, pp. 304-315.
Korzeniewska E, Harnisz M. Relationship between modification of activated sludge wastewater treatment and changes in antibiotic resistance of bacteria. Sci Total Environ. 2018;639:304-315.
Korzeniewska, E., & Harnisz, M. (2018). Relationship between modification of activated sludge wastewater treatment and changes in antibiotic resistance of bacteria. The Science of the Total Environment, 639, 304-315. https://doi.org/10.1016/j.scitotenv.2018.05.165
Korzeniewska E, Harnisz M. Relationship Between Modification of Activated Sludge Wastewater Treatment and Changes in Antibiotic Resistance of Bacteria. Sci Total Environ. 2018 Oct 15;639:304-315. PubMed PMID: 29791883.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between modification of activated sludge wastewater treatment and changes in antibiotic resistance of bacteria. AU - Korzeniewska,Ewa, AU - Harnisz,Monika, Y1 - 2018/05/20/ PY - 2018/03/01/received PY - 2018/05/13/revised PY - 2018/05/13/accepted PY - 2018/5/24/pubmed PY - 2019/2/6/medline PY - 2018/5/24/entrez KW - Activated sludge KW - Antibiotic resistance bacteria KW - Antibiotic resistance genes KW - Escherichia coli KW - Wastewater treatment plant KW - qPCR SP - 304 EP - 315 JF - The Science of the total environment JO - Sci Total Environ VL - 639 N2 - Biological treatment processes at wastewater treatment plants (WWTPs), which are the most common methods of sewage treatment, could cause selective elimination and/or changes in the proportions of phenotypes/genotypes within bacterial populations in effluent. Therefore, WWTPs based on activated sludge used in sewage treatment constitute an important reservoir of enteric bacteria which harbour potentially transferable resistance genes. Together with treated wastewater, these microorganisms can penetrate the soil, surface water, rural groundwater supplies and drinking water. Because of this, the aim of this study was to determine the impact of various modification of sewage treatment (the conventional anaerobic/anoxic/oxic (A2/O) process, mechanical-biological (MB) system, sequencing batch reactors (SBR), mechanical-biological system with elevated removal of nutrients (MB-ERN)) on the amount of antibiotic resistant bacteria (ARB) (including E. coli) and antibiotic resistance genes (ARGs) in sewage flowing out of the 13 treatment plants using activated sludge technology. There were no significant differences in ARB and ARGs regardless of time of sampling and type of treated wastewater (p > 0.05). The highest percentage of reduction (up to 99.9%) in the amount of ARB and ARGs was observed in WWTPs with MB and MB-ERN systems. The lowest reduction was detected in WWTPs with SBR. A significant increase (p < 0.05) in the percentage of bacteria resistant to the new generation antibiotics (CTX and DOX) in total counts of microorganisms was observed in effluents (EFF) from WWTPs with A2/O system and with SBR. Among all ARGs analyzed, the highest prevalence of ARGs copies in EFF samples was observed for sul1, tet(A) and qepA, the lowest for blaTEM and blaSHV. Although, the results of presented study demonstrate high efficiency of ARB and ARGs removal during the wastewater treatment processes, especially by WWTPs with MB and MB-ERN systems, EFF is still an important reservoir of ARGs which can be transferred to other microorganisms. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/29791883/Relationship_between_modification_of_activated_sludge_wastewater_treatment_and_changes_in_antibiotic_resistance_of_bacteria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(18)31813-8 DB - PRIME DP - Unbound Medicine ER -