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Bacillus subtilis as probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits.
Fish Shellfish Immunol. 2018 Aug; 79:303-312.FS

Abstract

The effects of dietary administration of Bacillus subtilis on the growth, digestive enzyme activity, blood chemistry, oxidative status and immune response of red sea bream (Pagrus major) were evaluated in the current study. Fish fed five different levels of B. subtilis at 0 (BS0), 1 × 104 (BS1), 1 × 106 (BS2), 1 × 108 (BS3) and 1 × 1010 (BS4) CFU kg-1 diet for 60 days. The obtained results showed that B. subtilis supplementation significantly improved growth performance (FBW, WG and SGR), feed utilization (FI, FCE, PER and PG) and whole-body protein content when compared to the control group (P < 0.05). Furthermore, the specific activities of amylase, protease and lipase enzymes up regulated significantly upon B. subtilis incorporation in red sea bream diets (P < 0.05). No changes have been reported on blood biochemical variables except for the plasma total protein, which increased significantly in fish fed BS3 diet when compared with the control diet (P < 0.05). Hematocrit, hemoglobin and the nitro blue tetrazolium values also reported the highest values significantly in fish fed B. subtilis, especially in case of BS3 and BS4 diets (P < 0.05). Serum bactericidal activity enhanced significantly in BS2, BS3 and BS4 groups (P < 0.05), while mucus bactericidal activity showed no significant activity among tested groups (P > 0.05). Serum lysozyme activity exhibited higher values in case of BS3 and BS4 groups than BS0 group (P < 0.05), while mucus lysozyme activity increased only in BS3 group. Also, serum peroxidase activity enhanced significantly in fish fed BS2 and BS3 diets (P < 0.05), however, no activities were observed in the collected mucus. All groups reflexed high tolerance ability against oxidative stress except for BS0 and BS1 groups. Additionally, catalase activity increased significantly in all B. subtilis fed groups when compared to BS0 group (P < 0.05). Considering the obtained results, the supplementation of B. subtilis in the diet of red sea bream at 1 × 108 and 1 × 1010 CFU kg-1 diet could improve the growth, feed utilization, health condition and immune response.

Authors+Show Affiliations

Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan; Animal Health Research Institute (AHRI-DOKI), Agriculture Research Center, Egypt.Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan.Department of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt.Animal Health Research Institute (AHRI-DOKI), Agriculture Research Center, Egypt.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan; Department of Animal Production, Faculty of Agriculture, Kafrelsheikh University, 33516, Kafrelsheikh, Egypt. Electronic address: mahmouddawood55@gmail.com.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan.Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, 4-50-20, Kagoshima, 890-0056, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29792927

Citation

Zaineldin, Amr I., et al. "Bacillus Subtilis as Probiotic Candidate for Red Sea Bream: Growth Performance, Oxidative Status, and Immune Response Traits." Fish & Shellfish Immunology, vol. 79, 2018, pp. 303-312.
Zaineldin AI, Hegazi S, Koshio S, et al. Bacillus subtilis as probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits. Fish Shellfish Immunol. 2018;79:303-312.
Zaineldin, A. I., Hegazi, S., Koshio, S., Ishikawa, M., Bakr, A., El-Keredy, A. M. S., Dawood, M. A. O., Dossou, S., Wang, W., & Yukun, Z. (2018). Bacillus subtilis as probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits. Fish & Shellfish Immunology, 79, 303-312. https://doi.org/10.1016/j.fsi.2018.05.035
Zaineldin AI, et al. Bacillus Subtilis as Probiotic Candidate for Red Sea Bream: Growth Performance, Oxidative Status, and Immune Response Traits. Fish Shellfish Immunol. 2018;79:303-312. PubMed PMID: 29792927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacillus subtilis as probiotic candidate for red sea bream: Growth performance, oxidative status, and immune response traits. AU - Zaineldin,Amr I, AU - Hegazi,Sayed, AU - Koshio,Shunsuke, AU - Ishikawa,Manabu, AU - Bakr,Abdulnasser, AU - El-Keredy,Abeer M S, AU - Dawood,Mahmoud A O, AU - Dossou,Serge, AU - Wang,Weilong, AU - Yukun,Zhang, Y1 - 2018/05/21/ PY - 2018/03/02/received PY - 2018/05/11/revised PY - 2018/05/18/accepted PY - 2018/5/25/pubmed PY - 2018/10/3/medline PY - 2018/5/25/entrez KW - Bacillus subtilis KW - Blood profile KW - Digestive enzymes KW - Growth KW - Immunity KW - Oxidative status KW - Probiotic KW - Red sea bream SP - 303 EP - 312 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol VL - 79 N2 - The effects of dietary administration of Bacillus subtilis on the growth, digestive enzyme activity, blood chemistry, oxidative status and immune response of red sea bream (Pagrus major) were evaluated in the current study. Fish fed five different levels of B. subtilis at 0 (BS0), 1 × 104 (BS1), 1 × 106 (BS2), 1 × 108 (BS3) and 1 × 1010 (BS4) CFU kg-1 diet for 60 days. The obtained results showed that B. subtilis supplementation significantly improved growth performance (FBW, WG and SGR), feed utilization (FI, FCE, PER and PG) and whole-body protein content when compared to the control group (P < 0.05). Furthermore, the specific activities of amylase, protease and lipase enzymes up regulated significantly upon B. subtilis incorporation in red sea bream diets (P < 0.05). No changes have been reported on blood biochemical variables except for the plasma total protein, which increased significantly in fish fed BS3 diet when compared with the control diet (P < 0.05). Hematocrit, hemoglobin and the nitro blue tetrazolium values also reported the highest values significantly in fish fed B. subtilis, especially in case of BS3 and BS4 diets (P < 0.05). Serum bactericidal activity enhanced significantly in BS2, BS3 and BS4 groups (P < 0.05), while mucus bactericidal activity showed no significant activity among tested groups (P > 0.05). Serum lysozyme activity exhibited higher values in case of BS3 and BS4 groups than BS0 group (P < 0.05), while mucus lysozyme activity increased only in BS3 group. Also, serum peroxidase activity enhanced significantly in fish fed BS2 and BS3 diets (P < 0.05), however, no activities were observed in the collected mucus. All groups reflexed high tolerance ability against oxidative stress except for BS0 and BS1 groups. Additionally, catalase activity increased significantly in all B. subtilis fed groups when compared to BS0 group (P < 0.05). Considering the obtained results, the supplementation of B. subtilis in the diet of red sea bream at 1 × 108 and 1 × 1010 CFU kg-1 diet could improve the growth, feed utilization, health condition and immune response. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/29792927/Bacillus_subtilis_as_probiotic_candidate_for_red_sea_bream:_Growth_performance_oxidative_status_and_immune_response_traits_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(18)30305-X DB - PRIME DP - Unbound Medicine ER -