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Male individualization using 12 rapidly mutating Y-STRs in Araein ethnic group and shared paternal lineage of Pakistani population.
Int J Legal Med. 2018 Nov; 132(6):1621-1624.IJ

Abstract

A multiplex assay has been developed with newly designed primer sets comprising high mutation rate 12 RM Y-STR markers (DYS570, DYF399S1, DYS547, DYS612, DYF387S1, DYS449, DYS576, DYS5626, DYF403S1 (a + b), DYS627, DYS526, and DYF404S1). Rapidly mutating Y-STRs were evaluated in 167 male individuals among 97 were unrelated from Araein ethnic group and 70 belonged to shared paternal lineage including 20 pairs of father-son and 15 pairs of brother-brother relationship collected from Punjabi population of Pakistan. Forensic competency parameters were implemented for each marker and exceptionally significant results found wherein polymorphism information content (PIC) was in range of 0.7494 (DYS576) to 0.8994 (DYS627). Samples were also analyzed with Y-filer kit for comparison and marked differentiations observed. Haplotype discrimination capacity was 100% as no haplotype shared among all the unrelated individuals of same ethnic group as compared to 17 Y-filer loci (78%). While in closely related males, discrimination capacity was 96.4% with haplotype diversity value of 0.98. Resulted high mutation rate 1 × 10-2 to 7.14 × 10-2 as compared to Y-filer (1 × 10-4 to 1 × 10-3) manifested the power of RM Y-STRs for considering absolute individualization of interrelated and unrelated male individuals. However, multiplex assay would be useful for male discrimination in mixed DNA specimen, azoospermic males, and multiple male DNA contributors in sexual assault cases and mass disasters victim's identification as well as anthropological studies.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan. shafique@cemb.edu.pk.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

De'montmorency College of Dentistry, Lahore, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

Forensic DNA Typing Laboratory, Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan.

MeSH

Chromosomes, Human, YDNA FingerprintingEthnicityGenetic MarkersGenetics, PopulationGenotypeHaplotypesHumansMaleMicrosatellite RepeatsMutation RatePakistanPolymerase Chain Reaction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29797282

Citation

Javed, Faqeeha, et al. "Male Individualization Using 12 Rapidly Mutating Y-STRs in Araein Ethnic Group and Shared Paternal Lineage of Pakistani Population." International Journal of Legal Medicine, vol. 132, no. 6, 2018, pp. 1621-1624.

Javed F, Sumbal S, Shafique M, et al. Male individualization using 12 rapidly mutating Y-STRs in Araein ethnic group and shared paternal lineage of Pakistani population. Int J Legal Med. 2018;132(6):1621-1624.

Javed, F., Sumbal, S., Shafique, M., Shahid, A. A., Shahzadi, A., Rani, N., Javid, H., Javed, M., Gillani, N., & Husnain, T. (2018). Male individualization using 12 rapidly mutating Y-STRs in Araein ethnic group and shared paternal lineage of Pakistani population. International Journal of Legal Medicine, 132(6), 1621-1624. https://doi.org/10.1007/s00414-018-1851-3

Javed F, et al. Male Individualization Using 12 Rapidly Mutating Y-STRs in Araein Ethnic Group and Shared Paternal Lineage of Pakistani Population. Int J Legal Med. 2018;132(6):1621-1624. PubMed PMID: 29797282.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Male individualization using 12 rapidly mutating Y-STRs in Araein ethnic group and shared paternal lineage of Pakistani population. AU - Javed,Faqeeha, AU - Sumbal,Sumbal, AU - Shafique,Muhammad, AU - Shahid,Ahmad Ali, AU - Shahzadi,Abida, AU - Rani,Noshaba, AU - Javid,Hira, AU - Javed,Madeeha, AU - Gillani,Noman, AU - Husnain,Tayyab, Y1 - 2018/05/24/ PY - 2017/08/28/received PY - 2018/05/11/accepted PY - 2018/5/26/pubmed PY - 2018/11/1/medline PY - 2018/5/26/entrez KW - Araein ethnic group KW - Haplotype diversity KW - Male individualization KW - Mutation rate KW - Paternal lineage KW - Rapidly mutating Y-STRs SP - 1621 EP - 1624 JF - International journal of legal medicine JO - Int J Legal Med VL - 132 IS - 6 N2 - A multiplex assay has been developed with newly designed primer sets comprising high mutation rate 12 RM Y-STR markers (DYS570, DYF399S1, DYS547, DYS612, DYF387S1, DYS449, DYS576, DYS5626, DYF403S1 (a + b), DYS627, DYS526, and DYF404S1). Rapidly mutating Y-STRs were evaluated in 167 male individuals among 97 were unrelated from Araein ethnic group and 70 belonged to shared paternal lineage including 20 pairs of father-son and 15 pairs of brother-brother relationship collected from Punjabi population of Pakistan. Forensic competency parameters were implemented for each marker and exceptionally significant results found wherein polymorphism information content (PIC) was in range of 0.7494 (DYS576) to 0.8994 (DYS627). Samples were also analyzed with Y-filer kit for comparison and marked differentiations observed. Haplotype discrimination capacity was 100% as no haplotype shared among all the unrelated individuals of same ethnic group as compared to 17 Y-filer loci (78%). While in closely related males, discrimination capacity was 96.4% with haplotype diversity value of 0.98. Resulted high mutation rate 1 × 10-2 to 7.14 × 10-2 as compared to Y-filer (1 × 10-4 to 1 × 10-3) manifested the power of RM Y-STRs for considering absolute individualization of interrelated and unrelated male individuals. However, multiplex assay would be useful for male discrimination in mixed DNA specimen, azoospermic males, and multiple male DNA contributors in sexual assault cases and mass disasters victim's identification as well as anthropological studies. SN - 1437-1596 UR - https://www.unboundmedicine.com/medline/citation/29797282/Male_individualization_using_12_rapidly_mutating_Y_STRs_in_Araein_ethnic_group_and_shared_paternal_lineage_of_Pakistani_population_ DB - PRIME DP - Unbound Medicine ER -