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[Effects of electroacupuncture on inflammatory response of cardiac muscle tissue in mice with acute myocardial ischemia].
Zhongguo Zhen Jiu. 2018 May 12; 38(5):5133-8.ZZ

Abstract

OBJECTIVE

To observe the effects of electroacupuncture (EA) on inflammatory reaction of acute myocardial ischemia (MI) in mice, and to explore its action mechanism.

METHODS

Forty adult male C57BL/6 mice were randomly divided into a control group, a sham operation group, a model group and an EA group, 10 mice in each one. The model was established in the model group and EA group by ligating the left anterior descending branch of coronary artery. The mice in the EA group were treated with EA at "Neiguan" (PC 6) with 2 mA of intensity and 2 Hz /100 Hz of frequency; EA was given 30 min per treatment, once a day for totally 5 days. The mice in the control group and model group were treated with immobilization and no EA was given. The mice in the sham operation group were not treated with ligating at the left anterior descending branch of coronary artery, but the remaining procedure was identical to the model group. The electrocardiogram was recorded and △ST was calculated to evaluate the model. TTC and HE staining methods were applied to evaluate the infarct size and pathologic change of myocardial tissue, respectively. Western blot method was applied to test the protein expression levels of tumor necrosis factor-α (TNF-α), nuclear factor-κB p65 (NF-κB p65), interleukin-1β (IL-1β) and interleukin-8 (IL-8).

RESULTS

Compared with the sham operation group, the S-T segments in the model group and EA group were increased obviously after modeling (both P<0.01), indicating the MI model was established successfully. The TTC and HE staining results indicated, compared with the sham operation group, the model group had larger infarction size (P<0.01), more myocardial fibers injury and inflammatory infiltration; compared with the model group, the infarction size of the EA group was significantly reduced (P<0.01), and the myocardial fibers injury and inflammatory infiltration were improved. Compared with the control group, the protein expression levels in the sham operation group were similar (all P>0.05); compared with the sham operation group, the expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 were significantly increased in the model group (P<0.01, P<0.05); compared with the model group, the expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 were significantly reduced in the EA group (all P<0.05).

CONCLUSION

EA might reduce the protein expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 in cardiac muscle tissue to inhibit inflammatory reaction and achieve myocardial protective effect in mice with acute myocardial ischemia.

Authors+Show Affiliations

Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.Ministry of Education Key Laboratory of Acupuncture and Medicine, Nanjing University of CM, Nanjing 210023, Jiangsu Province, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29797917

Citation

Wang, Junmeng, et al. "[Effects of Electroacupuncture On Inflammatory Response of Cardiac Muscle Tissue in Mice With Acute Myocardial Ischemia]." Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, vol. 38, no. 5, 2018, pp. 5133-8.
Wang J, Yuan J, Cai Y, et al. [Effects of electroacupuncture on inflammatory response of cardiac muscle tissue in mice with acute myocardial ischemia]. Zhongguo Zhen Jiu. 2018;38(5):5133-8.
Wang, J., Yuan, J., Cai, Y., Fu, S., Li, M., Hong, H., Lu, S., & Zhu, B. (2018). [Effects of electroacupuncture on inflammatory response of cardiac muscle tissue in mice with acute myocardial ischemia]. Zhongguo Zhen Jiu = Chinese Acupuncture & Moxibustion, 38(5), 5133-8. https://doi.org/10.13703/j.0255-2930.2018.05.017
Wang J, et al. [Effects of Electroacupuncture On Inflammatory Response of Cardiac Muscle Tissue in Mice With Acute Myocardial Ischemia]. Zhongguo Zhen Jiu. 2018 May 12;38(5):5133-8. PubMed PMID: 29797917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of electroacupuncture on inflammatory response of cardiac muscle tissue in mice with acute myocardial ischemia]. AU - Wang,Junmeng, AU - Yuan,Jing, AU - Cai,Yun, AU - Fu,Shuping, AU - Li,Minhui, AU - Hong,Hao, AU - Lu,Shengfeng, AU - Zhu,Bingmei, PY - 2018/5/26/entrez PY - 2018/5/26/pubmed PY - 2018/8/18/medline KW - Point PC 6 (Neiguan) KW - electroacupuncture (EA) KW - inflammatory reaction KW - interleukin-1β (IL-1β) KW - interleukin-8 (IL-8) KW - myocardial ischemia KW - nuclear factor-κB p65 (NF-κB p65) KW - tumor necrosis factor-α (TNF-α) SP - 5133 EP - 8 JF - Zhongguo zhen jiu = Chinese acupuncture & moxibustion JO - Zhongguo Zhen Jiu VL - 38 IS - 5 N2 - OBJECTIVE: To observe the effects of electroacupuncture (EA) on inflammatory reaction of acute myocardial ischemia (MI) in mice, and to explore its action mechanism. METHODS: Forty adult male C57BL/6 mice were randomly divided into a control group, a sham operation group, a model group and an EA group, 10 mice in each one. The model was established in the model group and EA group by ligating the left anterior descending branch of coronary artery. The mice in the EA group were treated with EA at "Neiguan" (PC 6) with 2 mA of intensity and 2 Hz /100 Hz of frequency; EA was given 30 min per treatment, once a day for totally 5 days. The mice in the control group and model group were treated with immobilization and no EA was given. The mice in the sham operation group were not treated with ligating at the left anterior descending branch of coronary artery, but the remaining procedure was identical to the model group. The electrocardiogram was recorded and △ST was calculated to evaluate the model. TTC and HE staining methods were applied to evaluate the infarct size and pathologic change of myocardial tissue, respectively. Western blot method was applied to test the protein expression levels of tumor necrosis factor-α (TNF-α), nuclear factor-κB p65 (NF-κB p65), interleukin-1β (IL-1β) and interleukin-8 (IL-8). RESULTS: Compared with the sham operation group, the S-T segments in the model group and EA group were increased obviously after modeling (both P<0.01), indicating the MI model was established successfully. The TTC and HE staining results indicated, compared with the sham operation group, the model group had larger infarction size (P<0.01), more myocardial fibers injury and inflammatory infiltration; compared with the model group, the infarction size of the EA group was significantly reduced (P<0.01), and the myocardial fibers injury and inflammatory infiltration were improved. Compared with the control group, the protein expression levels in the sham operation group were similar (all P>0.05); compared with the sham operation group, the expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 were significantly increased in the model group (P<0.01, P<0.05); compared with the model group, the expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 were significantly reduced in the EA group (all P<0.05). CONCLUSION: EA might reduce the protein expression levels of TNF-α, NF-κB p65, IL-1β and IL-8 in cardiac muscle tissue to inhibit inflammatory reaction and achieve myocardial protective effect in mice with acute myocardial ischemia. SN - 0255-2930 UR - https://www.unboundmedicine.com/medline/citation/29797917/[Effects_of_electroacupuncture_on_inflammatory_response_of_cardiac_muscle_tissue_in_mice_with_acute_myocardial_ischemia]_ DB - PRIME DP - Unbound Medicine ER -