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Multi-azole-resistant strain of Malassezia pachydermatis isolated from a canine Malassezia dermatitis.
Med Mycol. 2019 Apr 01; 57(3):346-350.MM

Abstract

In the case presented here, we describe the isolation of an azole-resistant strain of M. pachydermatis from a canine Malassezia dermatitis. The isolate (NUBS18001) from this case exhibited a minimum inhibitory concentration (MIC) of 320 μg/ml to itraconazole (ITZ) by broth microdilution (BM) assay, >32 μg/ml to ITZ by E-test, and >32 μg/ml to KTZ by E-test. Synergistic effects between FK506 and ITZ in the azole-resistant strain was evaluated using the microdilution checker-board method. The ITZ-resistant strain exhibited MICs of 320 μg/mL of ITZ alone and 5 μg/ml of FK506 alone; the addition of FK506 attenuated the ITZ MIC to 2.5 μg/ml, yielding an ITZ FICI value of 0.507. This result suggested that the combination of ITZ and FK506 exerted an additive effect against the ITZ-resistant strain. To understand the other mechanism inferred to be present in our multi-azole-resistant strain, we sequenced the ERG11 gene from this isolate, and detected missense mutations (A412G and C905T) in the sequence of the ERG11 open reading frame (ORF). To the best of our knowledge, this work is the first report that a multi-azole-resistant M. pacydermatis strain contains mutations in ERG11.

Authors+Show Affiliations

Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.Sennan Animal Hospital, 3-2297-1 Noda, Kumatori, Sennan, Osaka, 590-0451, Japan.Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.Department of Veterinary Pathobiology, Nihon University College of Bioresouce Sciences, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29800467

Citation

Kano, Rui, et al. "Multi-azole-resistant Strain of Malassezia Pachydermatis Isolated From a Canine Malassezia Dermatitis." Medical Mycology, vol. 57, no. 3, 2019, pp. 346-350.
Kano R, Yokoi S, Kariya N, et al. Multi-azole-resistant strain of Malassezia pachydermatis isolated from a canine Malassezia dermatitis. Med Mycol. 2019;57(3):346-350.
Kano, R., Yokoi, S., Kariya, N., Oshimo, K., & Kamata, H. (2019). Multi-azole-resistant strain of Malassezia pachydermatis isolated from a canine Malassezia dermatitis. Medical Mycology, 57(3), 346-350. https://doi.org/10.1093/mmy/myy035
Kano R, et al. Multi-azole-resistant Strain of Malassezia Pachydermatis Isolated From a Canine Malassezia Dermatitis. Med Mycol. 2019 Apr 1;57(3):346-350. PubMed PMID: 29800467.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multi-azole-resistant strain of Malassezia pachydermatis isolated from a canine Malassezia dermatitis. AU - Kano,Rui, AU - Yokoi,Shinichi, AU - Kariya,Naoki, AU - Oshimo,Karin, AU - Kamata,Hiroshi, PY - 2018/03/14/received PY - 2018/04/04/revised PY - 2018/05/02/accepted PY - 2018/5/26/pubmed PY - 2019/4/5/medline PY - 2018/5/26/entrez KW - ERG11 KW - Malassezia pachydermatis KW - itraconazol KW - mutation KW - resistance SP - 346 EP - 350 JF - Medical mycology JO - Med. Mycol. VL - 57 IS - 3 N2 - In the case presented here, we describe the isolation of an azole-resistant strain of M. pachydermatis from a canine Malassezia dermatitis. The isolate (NUBS18001) from this case exhibited a minimum inhibitory concentration (MIC) of 320 μg/ml to itraconazole (ITZ) by broth microdilution (BM) assay, >32 μg/ml to ITZ by E-test, and >32 μg/ml to KTZ by E-test. Synergistic effects between FK506 and ITZ in the azole-resistant strain was evaluated using the microdilution checker-board method. The ITZ-resistant strain exhibited MICs of 320 μg/mL of ITZ alone and 5 μg/ml of FK506 alone; the addition of FK506 attenuated the ITZ MIC to 2.5 μg/ml, yielding an ITZ FICI value of 0.507. This result suggested that the combination of ITZ and FK506 exerted an additive effect against the ITZ-resistant strain. To understand the other mechanism inferred to be present in our multi-azole-resistant strain, we sequenced the ERG11 gene from this isolate, and detected missense mutations (A412G and C905T) in the sequence of the ERG11 open reading frame (ORF). To the best of our knowledge, this work is the first report that a multi-azole-resistant M. pacydermatis strain contains mutations in ERG11. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/29800467/Multi_azole_resistant_strain_of_Malassezia_pachydermatis_isolated_from_a_canine_Malassezia_dermatitis_ L2 - https://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myy035 DB - PRIME DP - Unbound Medicine ER -