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A label-free photoelectrochemical biosensor for urokinase-type plasminogen activator detection based on a g-C3N4/CdS nanocomposite.
Anal Chim Acta. 2018 Sep 26; 1025:99-107.AC

Abstract

Herein, we established a novel ultrasensitive photoelectrochemical biosensor for detecting urokinase-type plasminogen activator (u-PA), based on a g-C3N4/CdS nanocomposite. The prepared nanocomposite was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, ultraviolet-visible absorption spectroscopy, and Fourier transform infrared spectroscopy, thus indicating that the nanocomposite was prepared successfully. In the typical process, the prepared nanocomposite was deposited on the surface of a bare FTO electrode. After being air-dried, the g-C3N4/CdS nanocomposite modified electrode was successively incubated with antibody against urokinase-type plasminogen activator and the blocking agent BSA to produce a photoelectrochemical biosensor for u-PA. In the presence of target u-PA antigen, the photocurrent response of the prepared biosensor electrode decreased significantly. The proposed novel photoelectrochemical biosensor exhibited good sensitivity, specificity, and reproducibility for u-PA detection, and a low detection limit of 33 fg mL-1, ranging from 1 μg mL-1-0.1 pg mL-1. The proposed strategy should provide a promising method for detection of other biomarkers.

Authors+Show Affiliations

Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China.Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China.Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China. Electronic address: maochangjie@sina.com.Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China.Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China.Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, School of Chemistry & Chemical Engineering, Anhui University, Hefei, 230601, China.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

29801612

Citation

Liu, Xing-Pei, et al. "A Label-free Photoelectrochemical Biosensor for Urokinase-type Plasminogen Activator Detection Based On a g-C3N4/CdS Nanocomposite." Analytica Chimica Acta, vol. 1025, 2018, pp. 99-107.
Liu XP, Chen JS, Mao CJ, et al. A label-free photoelectrochemical biosensor for urokinase-type plasminogen activator detection based on a g-C3N4/CdS nanocomposite. Anal Chim Acta. 2018;1025:99-107.
Liu, X. P., Chen, J. S., Mao, C. J., Niu, H. L., Song, J. M., & Jin, B. K. (2018). A label-free photoelectrochemical biosensor for urokinase-type plasminogen activator detection based on a g-C3N4/CdS nanocomposite. Analytica Chimica Acta, 1025, 99-107. https://doi.org/10.1016/j.aca.2018.04.051
Liu XP, et al. A Label-free Photoelectrochemical Biosensor for Urokinase-type Plasminogen Activator Detection Based On a g-C3N4/CdS Nanocomposite. Anal Chim Acta. 2018 Sep 26;1025:99-107. PubMed PMID: 29801612.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A label-free photoelectrochemical biosensor for urokinase-type plasminogen activator detection based on a g-C3N4/CdS nanocomposite. AU - Liu,Xing-Pei, AU - Chen,Jing-Shuai, AU - Mao,Chang-Jie, AU - Niu,He-Lin, AU - Song,Ji-Ming, AU - Jin,Bao-Kang, Y1 - 2018/04/23/ PY - 2018/02/15/received PY - 2018/04/16/revised PY - 2018/04/20/accepted PY - 2018/5/27/entrez PY - 2018/5/29/pubmed PY - 2018/9/27/medline KW - Biosensor KW - C(3)N(4)/CdS KW - Photoelectrochemistry KW - Urokinase-type plasminogen activator SP - 99 EP - 107 JF - Analytica chimica acta JO - Anal Chim Acta VL - 1025 N2 - Herein, we established a novel ultrasensitive photoelectrochemical biosensor for detecting urokinase-type plasminogen activator (u-PA), based on a g-C3N4/CdS nanocomposite. The prepared nanocomposite was characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, ultraviolet-visible absorption spectroscopy, and Fourier transform infrared spectroscopy, thus indicating that the nanocomposite was prepared successfully. In the typical process, the prepared nanocomposite was deposited on the surface of a bare FTO electrode. After being air-dried, the g-C3N4/CdS nanocomposite modified electrode was successively incubated with antibody against urokinase-type plasminogen activator and the blocking agent BSA to produce a photoelectrochemical biosensor for u-PA. In the presence of target u-PA antigen, the photocurrent response of the prepared biosensor electrode decreased significantly. The proposed novel photoelectrochemical biosensor exhibited good sensitivity, specificity, and reproducibility for u-PA detection, and a low detection limit of 33 fg mL-1, ranging from 1 μg mL-1-0.1 pg mL-1. The proposed strategy should provide a promising method for detection of other biomarkers. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/29801612/A_label_free_photoelectrochemical_biosensor_for_urokinase_type_plasminogen_activator_detection_based_on_a_g_C3N4/CdS_nanocomposite_ DB - PRIME DP - Unbound Medicine ER -