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Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma using Special Stains: An in vitro Histopathological Study.
J Contemp Dent Pract. 2018 May 01; 19(5):579-586.JC

Abstract

AIM

To evaluate the tissue eosinophilia in different stages and grades of oral squamous cell carcinoma (OSCC) and correlate its possible role as a prognosticator in primary OSCC using special stains like Congo red and carbol chromotrope.

MATERIALS AND METHODS

Forty-five intraoral histopathologi-cally proven cases of OSCC were selected (15 cases each of well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC), and poorly differentiated squamous cell carcinoma (PDSCC)). Three sections of 4 pm were taken for each case. All slides were stained using routine stain, i.e., hematoxylin and eosin (H&E), and special stains, i.e., carbol chromotrope and Congo red respectively, for studying tissue eosinophils in all these groups. Histopathological evaluation was performed on paraffin sections for calculating the quantitative eosinophil distribution by two separate observers who were blinded to clinical and histopathological data. Tumor-associated tissue eosinophilia (TATE) was calculated using the density method.

RESULTS

An increase in the degree of TATE from stage I to stage IV suggested that elevated tissue eosinophilia was seen with an increasing size of primary tumor. Also an increase in TATE was observed with an increasing grade of OSCC. Chromotrope stain was found to be a much better and more specific stain for eosinophils, and gave more accurate eosinophil count compared with H&E and Congo red.

CONCLUSION

The findings of the present study highlight the significance of eosinophil counting and that it can be used as an additional morphological parameter in the grading of OSCC which can also be included in the biopsy report.

Authors+Show Affiliations

Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India, Phone: +919923132047, e-mail: drvaibhavladke@gmail.com.Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India.Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India.Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India.Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India.Department of Oral Pathology and Microbiology, M.A. Rangoonwala College of Dental Science & Research Centre Pune, Maharashtra, India; Maharashtra University of Health Sciences, Nashik, Maharashtra, India.Department of Chemistry, Georgia State University, Atlanta Georgia, USA.Department of Biotechnology, Sinhgad College of Engineering Pune, Maharashtra, India.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29807970

Citation

Vaibhav, S Ladke, et al. "Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma Using Special Stains: an in Vitro Histopathological Study." The Journal of Contemporary Dental Practice, vol. 19, no. 5, 2018, pp. 579-586.
Vaibhav SL, Priya PL, Sonam CK, et al. Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma using Special Stains: An in vitro Histopathological Study. J Contemp Dent Pract. 2018;19(5):579-586.
Vaibhav, S. L., Priya, P. L., Sonam, C. K., Supriya, K., Garima, Y., Sabeer, S., Juvale, P., & Javir, G. (2018). Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma using Special Stains: An in vitro Histopathological Study. The Journal of Contemporary Dental Practice, 19(5), 579-586.
Vaibhav SL, et al. Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma Using Special Stains: an in Vitro Histopathological Study. J Contemp Dent Pract. 2018 May 1;19(5):579-586. PubMed PMID: 29807970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of Tumor-associated Tissue Eosinophilia in Different Stages of Oral Squamous Cell Carcinoma using Special Stains: An in vitro Histopathological Study. AU - Vaibhav,S Ladke, AU - Priya,P Lunawat, AU - Sonam,C Kapse, AU - Supriya,Koshti, AU - Garima,Yadav, AU - Sabeer,Shaikh, AU - Juvale,Parag, AU - Javir,Gitanjali, Y1 - 2018/05/01/ PY - 2018/5/30/entrez PY - 2018/5/29/pubmed PY - 2019/9/26/medline KW - Eosinophilia KW - Oral squamous cell carcinoma KW - Prognosis KW - Stains KW - Tumor stroma Tumor-associated tissue eosinophilia. SP - 579 EP - 586 JF - The journal of contemporary dental practice JO - J Contemp Dent Pract VL - 19 IS - 5 N2 - AIM: To evaluate the tissue eosinophilia in different stages and grades of oral squamous cell carcinoma (OSCC) and correlate its possible role as a prognosticator in primary OSCC using special stains like Congo red and carbol chromotrope. MATERIALS AND METHODS: Forty-five intraoral histopathologi-cally proven cases of OSCC were selected (15 cases each of well-differentiated squamous cell carcinoma (WDSCC), moderately differentiated squamous cell carcinoma (MDSCC), and poorly differentiated squamous cell carcinoma (PDSCC)). Three sections of 4 pm were taken for each case. All slides were stained using routine stain, i.e., hematoxylin and eosin (H&E), and special stains, i.e., carbol chromotrope and Congo red respectively, for studying tissue eosinophils in all these groups. Histopathological evaluation was performed on paraffin sections for calculating the quantitative eosinophil distribution by two separate observers who were blinded to clinical and histopathological data. Tumor-associated tissue eosinophilia (TATE) was calculated using the density method. RESULTS: An increase in the degree of TATE from stage I to stage IV suggested that elevated tissue eosinophilia was seen with an increasing size of primary tumor. Also an increase in TATE was observed with an increasing grade of OSCC. Chromotrope stain was found to be a much better and more specific stain for eosinophils, and gave more accurate eosinophil count compared with H&E and Congo red. CONCLUSION: The findings of the present study highlight the significance of eosinophil counting and that it can be used as an additional morphological parameter in the grading of OSCC which can also be included in the biopsy report. SN - 1526-3711 UR - https://www.unboundmedicine.com/medline/citation/29807970/Evaluation_of_Tumor_associated_Tissue_Eosinophilia_in_Different_Stages_of_Oral_Squamous_Cell_Carcinoma_using_Special_Stains:_An_in_vitro_Histopathological_Study_ L2 - http://www.diseaseinfosearch.org/result/1077 DB - PRIME DP - Unbound Medicine ER -