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Attenuated Junin virus infection in Callithrix jacchus.
J Med Virol. 1985 Jan; 15(1):93-100.JM

Abstract

Twenty marmosets, male Callithrix jacchus, were used during this study. Fifteen of the marmosets were inoculated with 5,000 TCID50 of the attenuated XJC13 strain of Junin virus by intramuscular route and five were left as uninoculated controls. Animals were observed for a 420-day period. In order to carry out virologic, hematologic, serologic, and histologic studies the animals were bled and/or killed at different days post infection(pi). Results obtained showed that the attenuated strain produced an infection with no mortality or signs of illness. There was only a slight loss of weight at 18-40 days pi, which was soon recovered. Viremia was present from day 6 to 22, titers peaking at 4.0 log. Viral spread was limited to the lungs, spleen, lymph nodes, and bone marrow in the animal killed on day 14. No virus was found in the organs of the animal killed on day 23, and neither hematologic alterations nor pathologic lesions were seen in these monkeys except for ganglionar hypertrophy with immunoblast proliferation. Antigen was detected by immunofluorescence (IF) in lymph nodes, spleen, adrenals, lungs and brain. Neutralizing antibodies were detected from the third week onward. Protection conferred by the XJC13 strain proved effective when XJC13-inoculated monkeys were challenged with 1,000 TCID50 of the pathogenic XJ strain at days 60 or 380 pi, while normal controls died. When viral persistence was searched for on days 370, 390, and 420 pi, no infectious virus was detected, but viral antigen was seen in certain organs, which, however, lacked tissue damage.(ABSTRACT TRUNCATED AT 250 WORDS)

Authors

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Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2981980

Citation

Avila, M M., et al. "Attenuated Junin Virus Infection in Callithrix Jacchus." Journal of Medical Virology, vol. 15, no. 1, 1985, pp. 93-100.
Avila MM, Frigerio MJ, Weber EL, et al. Attenuated Junin virus infection in Callithrix jacchus. J Med Virol. 1985;15(1):93-100.
Avila, M. M., Frigerio, M. J., Weber, E. L., Rondinone, S., Samoilovich, S. R., Laguens, R. P., de Guerrero, L. B., & Weissenbacher, M. C. (1985). Attenuated Junin virus infection in Callithrix jacchus. Journal of Medical Virology, 15(1), 93-100.
Avila MM, et al. Attenuated Junin Virus Infection in Callithrix Jacchus. J Med Virol. 1985;15(1):93-100. PubMed PMID: 2981980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Attenuated Junin virus infection in Callithrix jacchus. AU - Avila,M M, AU - Frigerio,M J, AU - Weber,E L, AU - Rondinone,S, AU - Samoilovich,S R, AU - Laguens,R P, AU - de Guerrero,L B, AU - Weissenbacher,M C, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 93 EP - 100 JF - Journal of medical virology JO - J Med Virol VL - 15 IS - 1 N2 - Twenty marmosets, male Callithrix jacchus, were used during this study. Fifteen of the marmosets were inoculated with 5,000 TCID50 of the attenuated XJC13 strain of Junin virus by intramuscular route and five were left as uninoculated controls. Animals were observed for a 420-day period. In order to carry out virologic, hematologic, serologic, and histologic studies the animals were bled and/or killed at different days post infection(pi). Results obtained showed that the attenuated strain produced an infection with no mortality or signs of illness. There was only a slight loss of weight at 18-40 days pi, which was soon recovered. Viremia was present from day 6 to 22, titers peaking at 4.0 log. Viral spread was limited to the lungs, spleen, lymph nodes, and bone marrow in the animal killed on day 14. No virus was found in the organs of the animal killed on day 23, and neither hematologic alterations nor pathologic lesions were seen in these monkeys except for ganglionar hypertrophy with immunoblast proliferation. Antigen was detected by immunofluorescence (IF) in lymph nodes, spleen, adrenals, lungs and brain. Neutralizing antibodies were detected from the third week onward. Protection conferred by the XJC13 strain proved effective when XJC13-inoculated monkeys were challenged with 1,000 TCID50 of the pathogenic XJ strain at days 60 or 380 pi, while normal controls died. When viral persistence was searched for on days 370, 390, and 420 pi, no infectious virus was detected, but viral antigen was seen in certain organs, which, however, lacked tissue damage.(ABSTRACT TRUNCATED AT 250 WORDS) SN - 0146-6615 UR - https://www.unboundmedicine.com/medline/citation/2981980/Attenuated_Junin_virus_infection_in_Callithrix_jacchus_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0146-6615&date=1985&volume=15&issue=1&spage=93 DB - PRIME DP - Unbound Medicine ER -