Tags

Type your tag names separated by a space and hit enter

The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia coli Strain MT78.
Infect Immun. 2018 08; 86(8)II

Abstract

Extraintestinal pathogenic Escherichia coli (ExPEC) is responsible for various infections outside the gastrointestinal tract in humans and other animals. ExPEC strain MT78 is invasive to various nonphagocytic cells and highly virulent in vivo To identify genes required for invasion of nonphagocytic cells by this strain, we applied signature-tagged mutagenesis to generate a library of mutants and tested them for invasion of avian fibroblasts. Mutants showing reduced cellular invasion included those with insertions in the fim operon, encoding type 1 fimbriae. Another attenuated mutant showed a disruption in the treA gene, which encodes a periplasmic trehalase. The substrate of TreA, trehalose, can be metabolized and used as a carbon source or can serve as an osmoprotectant under conditions of osmotic stress in E. coli K-12. We generated and characterized mutant MT78ΔtreA In contrast to the wild type, MT78ΔtreA was able to grow under osmotic stress caused by 0.6 M urea but not in minimal M9 medium with trehalose as the only carbon source. It presented decreased association and invasion of avian fibroblasts, decreased yeast agglutination titer, and impaired type 1 fimbria production. In a murine model of urinary tract infection, MT78ΔtreA was less able to colonize the bladder. All phenotypes were rescued in the complemented mutant. Our results show that the treA gene is needed for optimal production of type 1 fimbriae in ExPEC strain MT78 and that loss of treA significantly reduces its cell invasion capacity and colonization of the bladder in a murine model of urinary tract infection.

Authors+Show Affiliations

Departamento de Biofísica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. INRS-Institut Armand-Frappier, Laval, Quebec, Canada.INRS-Institut Armand-Frappier, Laval, Quebec, Canada.Departamento de Ciências da Saúde, Universidade Regional Integrada do Alto Uruguai e das Missões, Santo Ângelo, Rio Grande do Sul, Brazil.INRS-Institut Armand-Frappier, Laval, Quebec, Canada.Departamento de Biofísica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil fabiana.horn@ufrgs.br.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29844238

Citation

Pavanelo, Daniel Brisotto, et al. "The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia Coli Strain MT78." Infection and Immunity, vol. 86, no. 8, 2018.
Pavanelo DB, Houle S, Matter LB, et al. The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia coli Strain MT78. Infect Immun. 2018;86(8).
Pavanelo, D. B., Houle, S., Matter, L. B., Dozois, C. M., & Horn, F. (2018). The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia coli Strain MT78. Infection and Immunity, 86(8). https://doi.org/10.1128/IAI.00241-18
Pavanelo DB, et al. The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia Coli Strain MT78. Infect Immun. 2018;86(8) PubMed PMID: 29844238.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal Pathogenic Escherichia coli Strain MT78. AU - Pavanelo,Daniel Brisotto, AU - Houle,Sébastien, AU - Matter,Letícia Beatriz, AU - Dozois,Charles Martin, AU - Horn,Fabiana, Y1 - 2018/07/23/ PY - 2018/03/29/received PY - 2018/05/21/accepted PY - 2018/5/31/pubmed PY - 2019/3/5/medline PY - 2018/5/31/entrez KW - ExPEC KW - MT78 KW - TreA KW - avian fibroblasts KW - extraintestinal pathogenic E. coli KW - signature-tagged mutagenesis KW - trehalase KW - trehalose KW - type 1 fimbriae KW - urinary tract infection JF - Infection and immunity JO - Infect Immun VL - 86 IS - 8 N2 - Extraintestinal pathogenic Escherichia coli (ExPEC) is responsible for various infections outside the gastrointestinal tract in humans and other animals. ExPEC strain MT78 is invasive to various nonphagocytic cells and highly virulent in vivo To identify genes required for invasion of nonphagocytic cells by this strain, we applied signature-tagged mutagenesis to generate a library of mutants and tested them for invasion of avian fibroblasts. Mutants showing reduced cellular invasion included those with insertions in the fim operon, encoding type 1 fimbriae. Another attenuated mutant showed a disruption in the treA gene, which encodes a periplasmic trehalase. The substrate of TreA, trehalose, can be metabolized and used as a carbon source or can serve as an osmoprotectant under conditions of osmotic stress in E. coli K-12. We generated and characterized mutant MT78ΔtreA In contrast to the wild type, MT78ΔtreA was able to grow under osmotic stress caused by 0.6 M urea but not in minimal M9 medium with trehalose as the only carbon source. It presented decreased association and invasion of avian fibroblasts, decreased yeast agglutination titer, and impaired type 1 fimbria production. In a murine model of urinary tract infection, MT78ΔtreA was less able to colonize the bladder. All phenotypes were rescued in the complemented mutant. Our results show that the treA gene is needed for optimal production of type 1 fimbriae in ExPEC strain MT78 and that loss of treA significantly reduces its cell invasion capacity and colonization of the bladder in a murine model of urinary tract infection. SN - 1098-5522 UR - https://www.unboundmedicine.com/medline/citation/29844238/The_Periplasmic_Trehalase_Affects_Type_1_Fimbria_Production_and_Virulence_of_Extraintestinal_Pathogenic_Escherichia_coli_Strain_MT78_ L2 - http://iai.asm.org/cgi/pmidlookup?view=long&pmid=29844238 DB - PRIME DP - Unbound Medicine ER -