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Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain PUFA as the major lipid source in feed for Atlantic salmon (Salmo salar).
Br J Nutr. 2018 06; 119(12):1378-1392.BJ

Abstract

Facing a bottleneck in the growth of aquaculture, and a gap in the supply and demand of the highly beneficial n-3 long-chain PUFA (LC-PUFA), sustainable alternatives to traditional marine-based feeds are required. Therefore, in the present trial, a novel oil obtained from a genetically engineered oilseed crop, Camelina sativa, that supplied over 25 % n-3 LC-PUFA was tested as a sole dietary-added lipid source in Atlantic salmon (Salmo salar) feed. Three groups of fish were fed three experimental diets for 12 weeks with the same basal composition and containing 20 % added oil supplied by either a blend of fish oil and rapeseed oil (1:3) (COM) reflecting current commercial formulations, wild-type Camelina oil (WCO) or the novel transgenic Camelina oil (TCO). There were no negative effects on the growth, survival rate or health of the fish. The whole fish and flesh n-3 LC-PUFA levels were highest in fish fed TCO, with levels more than 2-fold higher compared with those of fish fed the COM and WCO diets, respectively. Diet TCO had no negative impacts on the evaluated immune and physiological parameters of head kidney monocytes. The transcriptomic responses of liver and mid-intestine showed only mild effects on metabolism genes. Overall, the results clearly indicated that the oil from transgenic Camelina was highly efficient in supplying n-3 LC-PUFA providing levels double that obtained with a current commercial standard, and similar to those a decade ago before substantial dietary fishmeal and oil replacement.

Authors+Show Affiliations

1Institute of Aquaculture, Faculty of Natural Sciences,University of Stirling,Stirling FK9 4LA,UK.2Department of Biology,Norwegian University of Science and Technology,7491 Trondheim,Norway.1Institute of Aquaculture, Faculty of Natural Sciences,University of Stirling,Stirling FK9 4LA,UK.1Institute of Aquaculture, Faculty of Natural Sciences,University of Stirling,Stirling FK9 4LA,UK.3Department of Biological Chemistry and Crop Protection,Rothamsted Research,Harpenden AL5 2JQ,UK.3Department of Biological Chemistry and Crop Protection,Rothamsted Research,Harpenden AL5 2JQ,UK.3Department of Biological Chemistry and Crop Protection,Rothamsted Research,Harpenden AL5 2JQ,UK.4Biomar AS,Havnegata 9,Pirsenteret 3,Trondheim 7010,Norway.5Institute of Marine Research,Matre 5984,Matredal,Norway.3Department of Biological Chemistry and Crop Protection,Rothamsted Research,Harpenden AL5 2JQ,UK.1Institute of Aquaculture, Faculty of Natural Sciences,University of Stirling,Stirling FK9 4LA,UK.2Department of Biology,Norwegian University of Science and Technology,7491 Trondheim,Norway.

Pub Type(s)

Clinical Trial, Veterinary
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29845899

Citation

Betancor, Mónica B., et al. "Oil From Transgenic Camelina Sativa Containing Over 25 % N-3 Long-chain PUFA as the Major Lipid Source in Feed for Atlantic Salmon (Salmo Salar)." The British Journal of Nutrition, vol. 119, no. 12, 2018, pp. 1378-1392.
Betancor MB, Li K, Bucerzan VS, et al. Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain PUFA as the major lipid source in feed for Atlantic salmon (Salmo salar). Br J Nutr. 2018;119(12):1378-1392.
Betancor, M. B., Li, K., Bucerzan, V. S., Sprague, M., Sayanova, O., Usher, S., Han, L., Norambuena, F., Torrissen, O., Napier, J. A., Tocher, D. R., & Olsen, R. E. (2018). Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain PUFA as the major lipid source in feed for Atlantic salmon (Salmo salar). The British Journal of Nutrition, 119(12), 1378-1392. https://doi.org/10.1017/S0007114518001125
Betancor MB, et al. Oil From Transgenic Camelina Sativa Containing Over 25 % N-3 Long-chain PUFA as the Major Lipid Source in Feed for Atlantic Salmon (Salmo Salar). Br J Nutr. 2018;119(12):1378-1392. PubMed PMID: 29845899.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oil from transgenic Camelina sativa containing over 25 % n-3 long-chain PUFA as the major lipid source in feed for Atlantic salmon (Salmo salar). AU - Betancor,Mónica B, AU - Li,Keshuai, AU - Bucerzan,Valentin S, AU - Sprague,Matthew, AU - Sayanova,Olga, AU - Usher,Sarah, AU - Han,Lihua, AU - Norambuena,Fernando, AU - Torrissen,Ole, AU - Napier,Johnathan A, AU - Tocher,Douglas R, AU - Olsen,Rolf E, PY - 2018/5/31/entrez PY - 2018/5/31/pubmed PY - 2019/4/10/medline KW - ADC apparent digestibility coefficient KW - COM fish oil/rapeseed oil feed KW - DEG differentially expressed genes KW - FC fold-change KW - FO fish oil KW - LC-PUFA n-3 long-chain PUFA KW - LPS lipopolysaccharide KW - PC pyloric caeca KW - TCO transgenic Camelina oil feed KW - VO vegetable oil KW - WCO wild-type Camelina oil feed KW - cDNA complementary DNA KW - Aquaculture KW - Camelina KW - DHA KW - EPA KW - Fish oil SP - 1378 EP - 1392 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 119 IS - 12 N2 - Facing a bottleneck in the growth of aquaculture, and a gap in the supply and demand of the highly beneficial n-3 long-chain PUFA (LC-PUFA), sustainable alternatives to traditional marine-based feeds are required. Therefore, in the present trial, a novel oil obtained from a genetically engineered oilseed crop, Camelina sativa, that supplied over 25 % n-3 LC-PUFA was tested as a sole dietary-added lipid source in Atlantic salmon (Salmo salar) feed. Three groups of fish were fed three experimental diets for 12 weeks with the same basal composition and containing 20 % added oil supplied by either a blend of fish oil and rapeseed oil (1:3) (COM) reflecting current commercial formulations, wild-type Camelina oil (WCO) or the novel transgenic Camelina oil (TCO). There were no negative effects on the growth, survival rate or health of the fish. The whole fish and flesh n-3 LC-PUFA levels were highest in fish fed TCO, with levels more than 2-fold higher compared with those of fish fed the COM and WCO diets, respectively. Diet TCO had no negative impacts on the evaluated immune and physiological parameters of head kidney monocytes. The transcriptomic responses of liver and mid-intestine showed only mild effects on metabolism genes. Overall, the results clearly indicated that the oil from transgenic Camelina was highly efficient in supplying n-3 LC-PUFA providing levels double that obtained with a current commercial standard, and similar to those a decade ago before substantial dietary fishmeal and oil replacement. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/29845899/Oil_from_transgenic_Camelina_sativa_containing_over_25__n_3_long_chain_PUFA_as_the_major_lipid_source_in_feed_for_Atlantic_salmon__Salmo_salar__ L2 - https://www.cambridge.org/core/product/identifier/S0007114518001125/type/journal_article DB - PRIME DP - Unbound Medicine ER -