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Antifungal susceptibilities, in vitro production of virulence factors and activities of ceragenins against Candida spp. isolated from vulvovaginal candidiasis.
Med Mycol 2019; 57(3):291-299MM

Abstract

Vulvovaginal candidiasis (VVC) is the second most common cause of vaginitis after bacterial vaginosis, affecting millions of women worldwide every year. Candida albicans is the most frequent agent of VVC followed by other species of Candida such as C. glabrata and C. parapsilosis. Out of a total of 100 clinical isolates of Candida spp. obtained from patients diagnosed with VVC, 84 were identified as C. albicans, while the remaining isolates were identified as non--albicans Candida strains. Phospholipases and proteinases were produced by a majority of the C. albicans strains and esterases and hemolysins a minority of these strains. Among the non-C. albicans strains, only a few of the strains produced these proteins. Nearly all of the isolates formed biofilms. Our results showed that the butoconazole, clotrimazole, and fluconazole were active against C. albicans and less so against the non-albicans Candida strains. The MIC90 of amphotericin B and nystatins were 2 and 4 μg/ml, respectively, against either C. albicans or non-albicans Candida spp. Representative ceragenins (CSA-13, CSA-131, and CSA-138), developed as mimics of endogenous antimicrobial peptides, were active against fluconazole-resistant strains, both alone and in combination with fluconazole. These results suggest the potential use of ceragenins in treating VVC, including infections caused by fluconazole-resistant isolates.

Authors+Show Affiliations

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, 34116, Turkey.Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, 34116, Turkey.Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, 84602, USA.Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Istanbul University, Beyazit, Istanbul, 34116, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29846682

Citation

Hacioglu, Mayram, et al. "Antifungal Susceptibilities, in Vitro Production of Virulence Factors and Activities of Ceragenins Against Candida Spp. Isolated From Vulvovaginal Candidiasis." Medical Mycology, vol. 57, no. 3, 2019, pp. 291-299.
Hacioglu M, Guzel CB, Savage PB, et al. Antifungal susceptibilities, in vitro production of virulence factors and activities of ceragenins against Candida spp. isolated from vulvovaginal candidiasis. Med Mycol. 2019;57(3):291-299.
Hacioglu, M., Guzel, C. B., Savage, P. B., & Tan, A. S. B. (2019). Antifungal susceptibilities, in vitro production of virulence factors and activities of ceragenins against Candida spp. isolated from vulvovaginal candidiasis. Medical Mycology, 57(3), pp. 291-299. doi:10.1093/mmy/myy023.
Hacioglu M, et al. Antifungal Susceptibilities, in Vitro Production of Virulence Factors and Activities of Ceragenins Against Candida Spp. Isolated From Vulvovaginal Candidiasis. Med Mycol. 2019 Apr 1;57(3):291-299. PubMed PMID: 29846682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antifungal susceptibilities, in vitro production of virulence factors and activities of ceragenins against Candida spp. isolated from vulvovaginal candidiasis. AU - Hacioglu,Mayram, AU - Guzel,Cagla Bozkurt, AU - Savage,Paul B, AU - Tan,A Seher Birteksoz, PY - 2017/12/25/received PY - 2018/03/01/revised PY - 2018/04/04/accepted PY - 2018/5/31/pubmed PY - 2019/4/5/medline PY - 2018/5/31/entrez KW - Candida spp KW - antifungal KW - ceragenin KW - virulence factors KW - vulvovaginal candidiasis SP - 291 EP - 299 JF - Medical mycology JO - Med. Mycol. VL - 57 IS - 3 N2 - Vulvovaginal candidiasis (VVC) is the second most common cause of vaginitis after bacterial vaginosis, affecting millions of women worldwide every year. Candida albicans is the most frequent agent of VVC followed by other species of Candida such as C. glabrata and C. parapsilosis. Out of a total of 100 clinical isolates of Candida spp. obtained from patients diagnosed with VVC, 84 were identified as C. albicans, while the remaining isolates were identified as non--albicans Candida strains. Phospholipases and proteinases were produced by a majority of the C. albicans strains and esterases and hemolysins a minority of these strains. Among the non-C. albicans strains, only a few of the strains produced these proteins. Nearly all of the isolates formed biofilms. Our results showed that the butoconazole, clotrimazole, and fluconazole were active against C. albicans and less so against the non-albicans Candida strains. The MIC90 of amphotericin B and nystatins were 2 and 4 μg/ml, respectively, against either C. albicans or non-albicans Candida spp. Representative ceragenins (CSA-13, CSA-131, and CSA-138), developed as mimics of endogenous antimicrobial peptides, were active against fluconazole-resistant strains, both alone and in combination with fluconazole. These results suggest the potential use of ceragenins in treating VVC, including infections caused by fluconazole-resistant isolates. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/29846682/Antifungal_susceptibilities,_in_vitro_production_of_virulence_factors_and_activities_of_ceragenins_against_Candida_spp._isolated_from_vulvovaginal_candidiasis L2 - https://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myy023 DB - PRIME DP - Unbound Medicine ER -