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Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine.
Infect Genet Evol. 2018 09; 63:257-268.IG

Abstract

The diagnosis of leishmaniasis relies mainly on the use of invasive processes, to collect the biological material for detecting Leishmania parasites. Body fluids, which can be collected by non-invasive process, would greatly facilitate the leishmaniasis diagnosis. In the present study, we investigated the potency of urine immunoblotting to diagnose cutaneous and visceral leishmaniasis and we compared with routine molecular methods. A total of 80 samples, including 40 sera and their 40 corresponding urine samples were collected from 37 suspected patients with cutaneous and visceral leishmaniasis, and 3 healthy individuals (as control), in Ilam and Ardabil provinces of Iran. All sera and urine samples were analyzed, using immunoblotting. The confirmation of leishmaniasis infection was performed, using conventional and quantitative PCRs as well as by sequencing the amplicons. Among 37 suspected patients, 23 patients presented cutaneous lesions (CL) and 14 exhibited clinical symptoms reminiscent of visceral leishmaniasis (L. infantum). Among cutaneous patients, 15 were positive for zoonotic cutaneous leishmaniasis (L. major), and eight for anthroponotic cutaneous leishmaniasis (L. tropica). Molecular quantification of Leishmania parasites was performed on sera, urines and cutaneous biopsies of CL and VL patients, demonstrating that parasite load is lower in urines, compared to sera or biopsy. DNA can be detected in 20 out of 23 (86.9%) CL urine samples and in 13 out of 14 (92.8%) VL urine samples. Immunodetection analysis demonstrates that 22 out of 23 (95.6%) sera from CL patients and all patients suspected with VL are positive. For urine samples, 18 out of 23 (78.2%) urine of CL patients and 13 out of 14 (92.8%) urine of VL patients were positive, using Western blot. Therefore, immunodetection and molecular analysis using urine samples can be used as a diagnostic tool for surveying cutaneous and visceral leishmaniasis.

Authors+Show Affiliations

Parasitology Department, Paramedical School, Ilam university of Medical Sciences, Iran.Department of Medical Entomology & Vector Control, Tehran University of Medical Sciences, Iran.Inserm U1065, Centre Méditerranéen de Médecine Moléculaire, C3 M, Université de Nice-Sophia Antipolis, 151, Route St Antoine de Ginestière, Cedex, BP 2 3194, 06204 Nice, France.Inserm U1065, Centre Méditerranéen de Médecine Moléculaire, C3 M, Université de Nice-Sophia Antipolis, 151, Route St Antoine de Ginestière, Cedex, BP 2 3194, 06204 Nice, France; Service Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice (CHU Nice), France; IRD, Montpellier University, MIVEGEC, Montpellier, France.Inserm U1065, Centre Méditerranéen de Médecine Moléculaire, C3 M, Université de Nice-Sophia Antipolis, 151, Route St Antoine de Ginestière, Cedex, BP 2 3194, 06204 Nice, France; Service Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice (CHU Nice), France; IRD, Montpellier University, MIVEGEC, Montpellier, France.IRD, Montpellier University, MIVEGEC, Montpellier, France.INRA-UMR 1062 CBGP (INRA, IRD, CIRAD), Montpellier SupAgro, Montferrier-sur-Lez, Languedoc- Roussillon 34988, France.IRD, Montpellier University, MIVEGEC, Montpellier, France; IRD, Montpellier University, InterTryp, Montpellier, France.Service Parasitologie-Mycologie, Centre Hospitalier Universitaire de Nice (CHU Nice), France; IRD, Montpellier University, MIVEGEC, Montpellier, France. Electronic address: m.akhoundi@yahoo.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29847780

Citation

Mirzaei, Asad, et al. "Immunodetection and Molecular Determination of Visceral and Cutaneous Leishmania Infection Using Patients' Urine." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 63, 2018, pp. 257-268.
Mirzaei A, Ahmadipour F, Cannet A, et al. Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine. Infect Genet Evol. 2018;63:257-268.
Mirzaei, A., Ahmadipour, F., Cannet, A., Marty, P., Delaunay, P., Perrin, P., Dorkeld, F., Sereno, D., & Akhoundi, M. (2018). Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 63, 257-268. https://doi.org/10.1016/j.meegid.2018.05.021
Mirzaei A, et al. Immunodetection and Molecular Determination of Visceral and Cutaneous Leishmania Infection Using Patients' Urine. Infect Genet Evol. 2018;63:257-268. PubMed PMID: 29847780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunodetection and molecular determination of visceral and cutaneous Leishmania infection using patients' urine. AU - Mirzaei,Asad, AU - Ahmadipour,Fereshteh, AU - Cannet,Arnaud, AU - Marty,Pierre, AU - Delaunay,Pascal, AU - Perrin,Pascale, AU - Dorkeld,Franck, AU - Sereno,Denis, AU - Akhoundi,Mohammad, Y1 - 2018/05/27/ PY - 2018/03/29/received PY - 2018/04/28/revised PY - 2018/05/22/accepted PY - 2018/5/31/pubmed PY - 2019/6/14/medline PY - 2018/5/31/entrez KW - Leishmaniasis KW - Serum KW - Urine KW - Western blot KW - qPCR SP - 257 EP - 268 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect Genet Evol VL - 63 N2 - The diagnosis of leishmaniasis relies mainly on the use of invasive processes, to collect the biological material for detecting Leishmania parasites. Body fluids, which can be collected by non-invasive process, would greatly facilitate the leishmaniasis diagnosis. In the present study, we investigated the potency of urine immunoblotting to diagnose cutaneous and visceral leishmaniasis and we compared with routine molecular methods. A total of 80 samples, including 40 sera and their 40 corresponding urine samples were collected from 37 suspected patients with cutaneous and visceral leishmaniasis, and 3 healthy individuals (as control), in Ilam and Ardabil provinces of Iran. All sera and urine samples were analyzed, using immunoblotting. The confirmation of leishmaniasis infection was performed, using conventional and quantitative PCRs as well as by sequencing the amplicons. Among 37 suspected patients, 23 patients presented cutaneous lesions (CL) and 14 exhibited clinical symptoms reminiscent of visceral leishmaniasis (L. infantum). Among cutaneous patients, 15 were positive for zoonotic cutaneous leishmaniasis (L. major), and eight for anthroponotic cutaneous leishmaniasis (L. tropica). Molecular quantification of Leishmania parasites was performed on sera, urines and cutaneous biopsies of CL and VL patients, demonstrating that parasite load is lower in urines, compared to sera or biopsy. DNA can be detected in 20 out of 23 (86.9%) CL urine samples and in 13 out of 14 (92.8%) VL urine samples. Immunodetection analysis demonstrates that 22 out of 23 (95.6%) sera from CL patients and all patients suspected with VL are positive. For urine samples, 18 out of 23 (78.2%) urine of CL patients and 13 out of 14 (92.8%) urine of VL patients were positive, using Western blot. Therefore, immunodetection and molecular analysis using urine samples can be used as a diagnostic tool for surveying cutaneous and visceral leishmaniasis. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/29847780/Immunodetection_and_molecular_determination_of_visceral_and_cutaneous_Leishmania_infection_using_patients'_urine_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(18)30309-5 DB - PRIME DP - Unbound Medicine ER -