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Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma.
Biomed Chromatogr 2018; 32(10):e4304BC

Abstract

Two selective and accurate chromatographic methods are presented for simultaneous quantitation of spironolactone (SP) and furosemide (FR) and canrenone (CN), the main degradation product and the main active metabolite of SP. Method A was HPTLC, where separation was completed on silica gel HPTLC F254 plates using ethyl acetate-triethylamine-acetic acid (9:0.7:0.5, by volume) as a developing system and UV detection at 254 nm. Method B was a green isocratic RP-HPLC utilizing a C18 (4.6 × 100 mm) column, the mobile phase consisting of ethanol-deionized water (45: 55, v/v) and UV estimation at 254 nm. Adjustment of flow rate at 1 mL/min and pH at 3.5 with glacial acetic acid was done. Regarding the greenness profile, the proposed RP-HPLC method is greener than the reported one. ICH guidelines were followed to validate the developed methods. Successful applications of the developed methods were revealed by simultaneous determination of FR, SP and CN in pure forms and plasma samples in the ranges of 0.2-2, 0.05-2.6 and 0.05-2 μg/band for method A and 5-60, 2-60 and 2-60 μg/mL for method B for FR, SP and CN, respectively.

Authors+Show Affiliations

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29855049

Citation

Naguib, Ibrahim A., et al. "Development and Validation of HPTLC and Green HPLC Methods for Determination of Furosemide, Spironolactone and Canrenone, in Pure Forms, Tablets and Spiked Human Plasma." Biomedical Chromatography : BMC, vol. 32, no. 10, 2018, pp. e4304.
Naguib IA, Abdelaleem EA, Emam AA, et al. Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma. Biomed Chromatogr. 2018;32(10):e4304.
Naguib, I. A., Abdelaleem, E. A., Emam, A. A., Ali, N. W., & Abdallah, F. F. (2018). Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma. Biomedical Chromatography : BMC, 32(10), pp. e4304. doi:10.1002/bmc.4304.
Naguib IA, et al. Development and Validation of HPTLC and Green HPLC Methods for Determination of Furosemide, Spironolactone and Canrenone, in Pure Forms, Tablets and Spiked Human Plasma. Biomed Chromatogr. 2018;32(10):e4304. PubMed PMID: 29855049.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and validation of HPTLC and green HPLC methods for determination of furosemide, spironolactone and canrenone, in pure forms, tablets and spiked human plasma. AU - Naguib,Ibrahim A, AU - Abdelaleem,Eglal A, AU - Emam,Aml A, AU - Ali,Nouruddin W, AU - Abdallah,Fatma F, Y1 - 2018/07/16/ PY - 2017/11/17/received PY - 2018/05/17/revised PY - 2018/05/23/accepted PY - 2018/6/2/pubmed PY - 2018/10/16/medline PY - 2018/6/2/entrez KW - HPLC KW - HPTLC KW - canrenone KW - furosemide KW - spironolactone KW - validation SP - e4304 EP - e4304 JF - Biomedical chromatography : BMC JO - Biomed. Chromatogr. VL - 32 IS - 10 N2 - Two selective and accurate chromatographic methods are presented for simultaneous quantitation of spironolactone (SP) and furosemide (FR) and canrenone (CN), the main degradation product and the main active metabolite of SP. Method A was HPTLC, where separation was completed on silica gel HPTLC F254 plates using ethyl acetate-triethylamine-acetic acid (9:0.7:0.5, by volume) as a developing system and UV detection at 254 nm. Method B was a green isocratic RP-HPLC utilizing a C18 (4.6 × 100 mm) column, the mobile phase consisting of ethanol-deionized water (45: 55, v/v) and UV estimation at 254 nm. Adjustment of flow rate at 1 mL/min and pH at 3.5 with glacial acetic acid was done. Regarding the greenness profile, the proposed RP-HPLC method is greener than the reported one. ICH guidelines were followed to validate the developed methods. Successful applications of the developed methods were revealed by simultaneous determination of FR, SP and CN in pure forms and plasma samples in the ranges of 0.2-2, 0.05-2.6 and 0.05-2 μg/band for method A and 5-60, 2-60 and 2-60 μg/mL for method B for FR, SP and CN, respectively. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/29855049/Development_and_validation_of_HPTLC_and_green_HPLC_methods_for_determination_of_furosemide_spironolactone_and_canrenone_in_pure_forms_tablets_and_spiked_human_plasma_ L2 - https://doi.org/10.1002/bmc.4304 DB - PRIME DP - Unbound Medicine ER -