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Stimulation by insulin-like growth factors is required for cellular transformation by type beta transforming growth factor.
J Biol Chem. 1985 Apr 25; 260(8):4551-4.JB

Abstract

Medium conditioned by BRL-3A cells, a known source of insulin-like growth factor II (IGF-II), induced phenotypic transformation (anchorage-independent proliferation) of mouse BALB/c 3T3 fibroblasts but not rat NRK-49F fibroblasts, in the presence of 10% calf serum. A specific radioreceptor assay and a bioassay indicated that BRL-3A conditioned medium contained 0.5-1 ng/ml of type beta transforming growth factor (beta TGF). Purified IGF-II and beta TGF acting together reconstituted the transforming activity of BRL-3A conditioned medium on BALB/c 3T3 cells. Insulin was 5-10% as potent as IGF-II in supporting the transforming action of beta TGF on BALB/c 3T3 cells. NRK-49F cells were phenotypically transformed by beta TGF in the presence of EGF and 10% calf serum as the sole source of IGFs. However, transformation of NRK-49F cells under these conditions was inhibited by addition of purified IGF-binding protein. Addition of an excess of IGF-II prevented the inhibitory action of IGF-binding protein. The different sensitivity of the two cell lines to IGFs was correlated with lower levels of type I IGF receptor and higher levels of type II IGF receptor in NRK-49F cells as compared with BALB/c 3T3 cells. The results suggest that cellular stimulation by IGFs is a prerequisite for transformation of rodent fibroblasts by beta TGF. We propose that transformation of fibroblasts by beta TGF requires concomitant stimulation by the set of growth factors that support normal cell proliferation.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2985553

Citation

Massagué, J, et al. "Stimulation By Insulin-like Growth Factors Is Required for Cellular Transformation By Type Beta Transforming Growth Factor." The Journal of Biological Chemistry, vol. 260, no. 8, 1985, pp. 4551-4.
Massagué J, Kelly B, Mottola C. Stimulation by insulin-like growth factors is required for cellular transformation by type beta transforming growth factor. J Biol Chem. 1985;260(8):4551-4.
Massagué, J., Kelly, B., & Mottola, C. (1985). Stimulation by insulin-like growth factors is required for cellular transformation by type beta transforming growth factor. The Journal of Biological Chemistry, 260(8), 4551-4.
Massagué J, Kelly B, Mottola C. Stimulation By Insulin-like Growth Factors Is Required for Cellular Transformation By Type Beta Transforming Growth Factor. J Biol Chem. 1985 Apr 25;260(8):4551-4. PubMed PMID: 2985553.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stimulation by insulin-like growth factors is required for cellular transformation by type beta transforming growth factor. AU - Massagué,J, AU - Kelly,B, AU - Mottola,C, PY - 1985/4/25/pubmed PY - 1985/4/25/medline PY - 1985/4/25/entrez SP - 4551 EP - 4 JF - The Journal of biological chemistry JO - J. Biol. Chem. VL - 260 IS - 8 N2 - Medium conditioned by BRL-3A cells, a known source of insulin-like growth factor II (IGF-II), induced phenotypic transformation (anchorage-independent proliferation) of mouse BALB/c 3T3 fibroblasts but not rat NRK-49F fibroblasts, in the presence of 10% calf serum. A specific radioreceptor assay and a bioassay indicated that BRL-3A conditioned medium contained 0.5-1 ng/ml of type beta transforming growth factor (beta TGF). Purified IGF-II and beta TGF acting together reconstituted the transforming activity of BRL-3A conditioned medium on BALB/c 3T3 cells. Insulin was 5-10% as potent as IGF-II in supporting the transforming action of beta TGF on BALB/c 3T3 cells. NRK-49F cells were phenotypically transformed by beta TGF in the presence of EGF and 10% calf serum as the sole source of IGFs. However, transformation of NRK-49F cells under these conditions was inhibited by addition of purified IGF-binding protein. Addition of an excess of IGF-II prevented the inhibitory action of IGF-binding protein. The different sensitivity of the two cell lines to IGFs was correlated with lower levels of type I IGF receptor and higher levels of type II IGF receptor in NRK-49F cells as compared with BALB/c 3T3 cells. The results suggest that cellular stimulation by IGFs is a prerequisite for transformation of rodent fibroblasts by beta TGF. We propose that transformation of fibroblasts by beta TGF requires concomitant stimulation by the set of growth factors that support normal cell proliferation. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/2985553/Stimulation_by_insulin_like_growth_factors_is_required_for_cellular_transformation_by_type_beta_transforming_growth_factor_ L2 - http://www.jbc.org/cgi/pmidlookup?view=long&pmid=2985553 DB - PRIME DP - Unbound Medicine ER -