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The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite.
Res Microbiol. 2018 Jul - Aug; 169(6):263-278.RM

Abstract

Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions.

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Authors+Show Affiliations

Cabezas CE
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Briones AC
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Aguirre C
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Pardo-Esté C
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Castro-Severyn J
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Salinas CR
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Baquedano MS
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile.
Hidalgo AA
Facultad de Medicina, Laboratorio de Patogénesis molecular y antimicrobianos, Universidad Nacional Andrés Bello, Santiago, Chile.
Fuentes JA
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Genética y Patogénesis Bacteriana, Universidad Nacional Andrés Bello, Santiago, Chile.
Morales EH
uBiome, Inc., San Francisco, CA, United State of America.
Meneses CA
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Centro de Biotecnología Vegetal, Universidad Nacional Andrés Bello, Santiago, Chile.
Castro-Nallar E
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Center for Bioinformatics and Integrative Biology, Universidad Nacional Andrés Bello, Santiago, Chile.
Saavedra CP
Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Laboratorio de Microbiología Molecular, Universidad Nacional Andrés Bello, Santiago, Chile; Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Millennium Institute on Immunology and Immunotherapy, Universidad Nacional Andrés Bello, Santiago, Chile. Electronic address: csaavedra@unab.cl.

MeSH

AnimalsBacterial ProteinsCell LineDicarboxylic Acid TransportersGene Expression ProfilingHydrogen PeroxideMiceOxidantsPhosphofructokinase-1Promoter Regions, GeneticRAW 264.7 CellsSalmonella InfectionsSalmonella typhimuriumSodium HypochloriteTrans-ActivatorsTranscription FactorsVirulence

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29857034

Citation

Cabezas, Carolina E., et al. "The Transcription Factor SlyA From Salmonella Typhimurium Regulates Genes in Response to Hydrogen Peroxide and Sodium Hypochlorite." Research in Microbiology, vol. 169, no. 6, 2018, pp. 263-278.
Cabezas CE, Briones AC, Aguirre C, et al. The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite. Res Microbiol. 2018;169(6):263-278.
Cabezas, C. E., Briones, A. C., Aguirre, C., Pardo-Esté, C., Castro-Severyn, J., Salinas, C. R., Baquedano, M. S., Hidalgo, A. A., Fuentes, J. A., Morales, E. H., Meneses, C. A., Castro-Nallar, E., & Saavedra, C. P. (2018). The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite. Research in Microbiology, 169(6), 263-278. https://doi.org/10.1016/j.resmic.2018.04.003
Cabezas CE, et al. The Transcription Factor SlyA From Salmonella Typhimurium Regulates Genes in Response to Hydrogen Peroxide and Sodium Hypochlorite. Res Microbiol. 2018 Jul - Aug;169(6):263-278. PubMed PMID: 29857034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The transcription factor SlyA from Salmonella Typhimurium regulates genes in response to hydrogen peroxide and sodium hypochlorite. AU - Cabezas,Carolina E, AU - Briones,Alan C, AU - Aguirre,Camila, AU - Pardo-Esté,Coral, AU - Castro-Severyn,Juan, AU - Salinas,César R, AU - Baquedano,María S, AU - Hidalgo,Alejandro A, AU - Fuentes,Juan A, AU - Morales,Eduardo H, AU - Meneses,Claudio A, AU - Castro-Nallar,Eduardo, AU - Saavedra,Claudia Paz, Y1 - 2018/05/29/ PY - 2017/06/12/received PY - 2018/03/29/revised PY - 2018/04/21/accepted PY - 2018/6/2/pubmed PY - 2018/10/26/medline PY - 2018/6/2/entrez KW - Gene regulation KW - Oxidative stress KW - RNAseq KW - ROS KW - Salmonella KW - SlyA SP - 263 EP - 278 JF - Research in microbiology JO - Res Microbiol VL - 169 IS - 6 N2 - Salmonella Typhimurium is an intracellular pathogen that is capable of generating systemic fever in a murine model. Over the course of the infection, Salmonella faces different kinds of stressors, including harmful reactive oxygen species (ROS). Various defence mechanisms enable Salmonella to successfully complete the infective process in the presence of such stressors. The transcriptional factor SlyA is involved in the oxidative stress response and invasion of murine macrophages. We evaluated the role of SlyA in response to H2O2 and NaOCl and found an increase of slyA expression upon exposure to these toxics. However, the SlyA target genes and the molecular mechanisms by which they influence the infective process are unknown. We hypothesised that SlyA regulates the expression of genes required for ROS resistance, metabolism, or virulence under oxidative stress conditions. Transcriptional profiling in wild type and ΔslyA strains confirmed that SlyA regulates the expression of several genes involved in virulence [sopD (STM14_3550), sopE2 (STM14_2244), hilA (STM14_3475)] and central metabolism [kgtP (STM14_3252), fruK (STM14_2722), glpA (STM14_2819)] in response to H2O2 and NaOCl. These findings were corroborated by functional assay and transcriptional fusion assays using GFP. DNA-protein interaction assays showed that SlyA regulates these genes through direct interaction with their promoter regions. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/29857034/The_transcription_factor_SlyA_from_Salmonella_Typhimurium_regulates_genes_in_response_to_hydrogen_peroxide_and_sodium_hypochlorite_ DB - PRIME DP - Unbound Medicine ER -