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Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells.
Life Sci 2018; 208:315-324LS

Abstract

AIMS

Hydrogen sulfide (H2S) is a novel signaling molecule with potent cytoprotective actions. In this study, we hypothesize that exogenous H2S may protect cardiac cells against high glucose (HG)-induced myocardial injury and inflammation with the involvement of the CIRP-MAPK signaling pathway.

MAIN METHODS

H9c2 cardiac cells cultured under HG conditions were transfected with siRNA and different inhibitor for detecting the effects of sodium hydrogen sulfide (NaHS) (a H2S donor) on cell biological processes. The cardiac cell viability and LDH activity were determined by CCK-8 and LDH kit. ELISA was employed to measure the levels of inflammatory factors, while 2',7'-dichlorofluorescein diacetate (DCFH-DA) to evaluate reactive oxygen species (ROS). Mitochondrial membrane potential (MMP) was identified by rhodamine 123 staining. TUNEL staining and Hoechst 33258 staining were employed to observe cardiac cell apoptosis. Besides, we determined the expression of CIRP-MAPK signaling pathway- and apoptosis-related factors by protein immunoblot analysis.

KEY FINDINGS

HG culturing induced toxicity, LDH, higher level of inflammatory factors, ROS, MMP, and apoptosis in cardiac cells, attenuated the viability of cardiac cells, and activated the CIRP-MAPK signaling pathway. Notably, CIRP silencing aggravated the above condition. H2S or blockade of the MAPK signaling pathway reversed the above conditions induced by HG.

SIGNIFICANCE

The present study provides evidence for the protective effect of exogenous H2S on HG-induced myocardial injury and inflammation in H9c2 cardiac cells and suggests that the activation of CIRP-MAPK signaling pathway might be one of the mechanisms underlying the protective effect of H2S.

Authors+Show Affiliations

Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China.Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, PR China.Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, PR China.Department of Geriatrics and Cardiovascular Medicine, Shenzhen Sun Yat-Sen Cardiovascular Hospital, Shenzhen 518112, PR China. Electronic address: xhchen66@126.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29857073

Citation

Zhao, Hong-Lei, et al. "Exogenous Hydrogen Sulfide Ameliorates High Glucose-induced Myocardial Injury & Inflammation Via the CIRP-MAPK Signaling Pathway in H9c2 Cardiac Cells." Life Sciences, vol. 208, 2018, pp. 315-324.
Zhao HL, Wu BQ, Luo Y, et al. Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. Life Sci. 2018;208:315-324.
Zhao, H. L., Wu, B. Q., Luo, Y., Zhang, W. Y., Hao, Y. L., Liang, J. J., ... Chen, X. H. (2018). Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. Life Sciences, 208, pp. 315-324. doi:10.1016/j.lfs.2018.05.051.
Zhao HL, et al. Exogenous Hydrogen Sulfide Ameliorates High Glucose-induced Myocardial Injury & Inflammation Via the CIRP-MAPK Signaling Pathway in H9c2 Cardiac Cells. Life Sci. 2018 Sep 1;208:315-324. PubMed PMID: 29857073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exogenous hydrogen sulfide ameliorates high glucose-induced myocardial injury & inflammation via the CIRP-MAPK signaling pathway in H9c2 cardiac cells. AU - Zhao,Hong-Lei, AU - Wu,Bao-Quan, AU - Luo,Ying, AU - Zhang,Wen-Ying, AU - Hao,Yun-Ling, AU - Liang,Jin-Jie, AU - Fang,Fang, AU - Liu,Wei, AU - Chen,Xie-Hui, Y1 - 2018/05/29/ PY - 2017/12/23/received PY - 2018/05/14/revised PY - 2018/05/28/accepted PY - 2018/6/2/pubmed PY - 2018/9/5/medline PY - 2018/6/2/entrez KW - Cardiac cells KW - Cold-inducible RNA-binding protein-mitogen-activated protein kinase signaling pathway KW - Exogenous hydrogen sulfide KW - High glucose KW - Inflammation KW - Myocardial injury SP - 315 EP - 324 JF - Life sciences JO - Life Sci. VL - 208 N2 - AIMS: Hydrogen sulfide (H2S) is a novel signaling molecule with potent cytoprotective actions. In this study, we hypothesize that exogenous H2S may protect cardiac cells against high glucose (HG)-induced myocardial injury and inflammation with the involvement of the CIRP-MAPK signaling pathway. MAIN METHODS: H9c2 cardiac cells cultured under HG conditions were transfected with siRNA and different inhibitor for detecting the effects of sodium hydrogen sulfide (NaHS) (a H2S donor) on cell biological processes. The cardiac cell viability and LDH activity were determined by CCK-8 and LDH kit. ELISA was employed to measure the levels of inflammatory factors, while 2',7'-dichlorofluorescein diacetate (DCFH-DA) to evaluate reactive oxygen species (ROS). Mitochondrial membrane potential (MMP) was identified by rhodamine 123 staining. TUNEL staining and Hoechst 33258 staining were employed to observe cardiac cell apoptosis. Besides, we determined the expression of CIRP-MAPK signaling pathway- and apoptosis-related factors by protein immunoblot analysis. KEY FINDINGS: HG culturing induced toxicity, LDH, higher level of inflammatory factors, ROS, MMP, and apoptosis in cardiac cells, attenuated the viability of cardiac cells, and activated the CIRP-MAPK signaling pathway. Notably, CIRP silencing aggravated the above condition. H2S or blockade of the MAPK signaling pathway reversed the above conditions induced by HG. SIGNIFICANCE: The present study provides evidence for the protective effect of exogenous H2S on HG-induced myocardial injury and inflammation in H9c2 cardiac cells and suggests that the activation of CIRP-MAPK signaling pathway might be one of the mechanisms underlying the protective effect of H2S. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/29857073/Exogenous_hydrogen_sulfide_ameliorates_high_glucose_induced_myocardial_injury_&_inflammation_via_the_CIRP_MAPK_signaling_pathway_in_H9c2_cardiac_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(18)30319-9 DB - PRIME DP - Unbound Medicine ER -