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Expression of CD206 and CD163 on intermediate CD14++CD16+ monocytes are increased in hemorrhagic fever with renal syndrome and are correlated with disease severity.
Virus Res. 2018 07 15; 253:92-102.VR

Abstract

BACKGROUND

Hantaan virus infection causes lethal hemorrhagic fever with renal syndrome (HFRS) in humans. Little is known about how monocytes contribute to HFRS pathogenesis. In this study, we aimed to investigate changes in various monocyte subsets in HFRS patients.

METHODS

A total of 41 HFRS patients and 17 age-, sex-, and ethnicity-matched healthy control subjects were included in this study. Numbers/percentages of various monocyte subsets were quantitatively determined using flow cytometry. Serum levels of interleukin (IL)-10, IL-12, and tumor necrosis factor alpha (TNF-α) were detected using a cytometric bead array (CBA).

RESULTS

CD14++CD16+ intermediate monocytes were significantly higher in HFRS patients compared to healthy controls (P < 0.01), especially during the acute phase. The expression of both CD163 and CD206 on CD14++CD16+ intermediate monocytes were increased during the acute phase of HFRS (P < 0.01 and P < 0.05, respectively) when comparing the convalescent phase and healthy controls. Furthermore, the numbers of CD14++CD16+ monocytes during the acute phase, and the percentages of CD14++CD16+CD163+ monocytes in patients with severe/critical HFRS were much higher compared to patients with mild/moderate HFRS. This also positively correlated with increased levels of white blood cells (WBC), blood urea nitrogen (BUN), and creatinine (Cr). However, the percentages of CD14++CD16+CD206+monocytes were higher in mild/moderate HFRS than in severe/critical HFRS, and they negatively correlated with platelets (PLT) and Cr.

CONCLUSIONS

Higher frequency of the CD14++CD16+ intermediate monocytes and increased expression of CD163+ and CD206+ markers on CD14++CD16+ monocytes were detected in patients with HFRS. The changes in the frequency of CD14++CD16+ monocytes and expression of CD163 and CD206 markers on CD14++CD16+ monocytes positively correlated with the severity of HFRS.

Authors+Show Affiliations

Department of Infectious Diseases, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: lixiaohua2018@hotmail.com.Department of Infectious Diseases, The First Hospital of Jilin University, Changchun 130021, China.Department of Infectious Diseases, The First Hospital of Jilin University, Changchun 130021, China.Department of Infectious Diseases, The First Hospital of Jilin University, Changchun 130021, China.Key Laboratory of Zoonoses Research, Ministry of Education, The First Hospital of Jilin University, Changchun 130021, China.Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun 130021, China; Key Laboratory of Zoonoses Research, Ministry of Education, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: yanfangjiang@hotmail.com.Department of Infectious Diseases, The First Hospital of Jilin University, Changchun 130021, China. Electronic address: zhangky2000@aliyun.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29857122

Citation

Li, XiaoHua, et al. "Expression of CD206 and CD163 On Intermediate CD14++CD16+ Monocytes Are Increased in Hemorrhagic Fever With Renal Syndrome and Are Correlated With Disease Severity." Virus Research, vol. 253, 2018, pp. 92-102.
Li X, Du N, Xu G, et al. Expression of CD206 and CD163 on intermediate CD14++CD16+ monocytes are increased in hemorrhagic fever with renal syndrome and are correlated with disease severity. Virus Res. 2018;253:92-102.
Li, X., Du, N., Xu, G., Zhang, P., Dang, R., Jiang, Y., & Zhang, K. (2018). Expression of CD206 and CD163 on intermediate CD14++CD16+ monocytes are increased in hemorrhagic fever with renal syndrome and are correlated with disease severity. Virus Research, 253, 92-102. https://doi.org/10.1016/j.virusres.2018.05.021
Li X, et al. Expression of CD206 and CD163 On Intermediate CD14++CD16+ Monocytes Are Increased in Hemorrhagic Fever With Renal Syndrome and Are Correlated With Disease Severity. Virus Res. 2018 07 15;253:92-102. PubMed PMID: 29857122.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of CD206 and CD163 on intermediate CD14++CD16+ monocytes are increased in hemorrhagic fever with renal syndrome and are correlated with disease severity. AU - Li,XiaoHua, AU - Du,Na, AU - Xu,Guang, AU - Zhang,Peng, AU - Dang,Rongjing, AU - Jiang,Yanfang, AU - Zhang,Kaiyu, Y1 - 2018/05/29/ PY - 2018/02/24/received PY - 2018/05/28/revised PY - 2018/05/28/accepted PY - 2018/6/2/pubmed PY - 2018/10/24/medline PY - 2018/6/2/entrez KW - Cytokine KW - Hemorrhagic fever with renal syndrome (HFRS) KW - Monocyte SP - 92 EP - 102 JF - Virus research JO - Virus Res. VL - 253 N2 - BACKGROUND: Hantaan virus infection causes lethal hemorrhagic fever with renal syndrome (HFRS) in humans. Little is known about how monocytes contribute to HFRS pathogenesis. In this study, we aimed to investigate changes in various monocyte subsets in HFRS patients. METHODS: A total of 41 HFRS patients and 17 age-, sex-, and ethnicity-matched healthy control subjects were included in this study. Numbers/percentages of various monocyte subsets were quantitatively determined using flow cytometry. Serum levels of interleukin (IL)-10, IL-12, and tumor necrosis factor alpha (TNF-α) were detected using a cytometric bead array (CBA). RESULTS: CD14++CD16+ intermediate monocytes were significantly higher in HFRS patients compared to healthy controls (P < 0.01), especially during the acute phase. The expression of both CD163 and CD206 on CD14++CD16+ intermediate monocytes were increased during the acute phase of HFRS (P < 0.01 and P < 0.05, respectively) when comparing the convalescent phase and healthy controls. Furthermore, the numbers of CD14++CD16+ monocytes during the acute phase, and the percentages of CD14++CD16+CD163+ monocytes in patients with severe/critical HFRS were much higher compared to patients with mild/moderate HFRS. This also positively correlated with increased levels of white blood cells (WBC), blood urea nitrogen (BUN), and creatinine (Cr). However, the percentages of CD14++CD16+CD206+monocytes were higher in mild/moderate HFRS than in severe/critical HFRS, and they negatively correlated with platelets (PLT) and Cr. CONCLUSIONS: Higher frequency of the CD14++CD16+ intermediate monocytes and increased expression of CD163+ and CD206+ markers on CD14++CD16+ monocytes were detected in patients with HFRS. The changes in the frequency of CD14++CD16+ monocytes and expression of CD163 and CD206 markers on CD14++CD16+ monocytes positively correlated with the severity of HFRS. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/29857122/Expression_of_CD206_and_CD163_on_intermediate_CD14++CD16+_monocytes_are_increased_in_hemorrhagic_fever_with_renal_syndrome_and_are_correlated_with_disease_severity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(18)30121-7 DB - PRIME DP - Unbound Medicine ER -