Tags

Type your tag names separated by a space and hit enter

Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.).
BMC Plant Biol. 2018 Jun 01; 18(1):100.BP

Abstract

BACKGROUND

Gibberellin (GA) treatments can induce parthenocarpy in the main crop of San Pedro-type figs, the native non-parthenocarpic fruit, however, the underlying mechanism is still largely unclear.

RESULTS

In our study, GA3 was applied to San Pedro-type fig main crop at anthesis. Sharply increased GA3 content was detected in both female flowers and receptacle, along with significantly decreased indole-3-acetic acid (IAA), zeatin and abscisic acid (ABA) levels in female flowers, and increased zeatin peak intensity and earlier ABA peak in receptacles. Transcriptome comparison between control and treatment groups identified more differentially expressed genes (DEGs) in receptacles than in female flowers 2 and 4 days after treatment (DAT); 10 DAT, the number of DEGs became similar in the two tissues. Synchronized changing trends of phytohormone-associated DEGs were observed in female flowers and receptacles with fruit development. Modulation of ethylene and GA signaling and auxin metabolism by exogenous GA3 occurred mainly 2 DAT, whereas changes in auxin, cytokinin and ABA signaling occurred mainly 10 DAT. Auxin-, ethylene- and ABA-metabolism and response pathways were largely regulated in the two tissues, mostly 2 and 10 DAT. The major components altering fig phytohormone metabolic and response patterns included downregulated GA2ox, BAS1, NCED and ACO, and upregulated ABA 8'-h and AUX/IAA.

CONCLUSIONS

Thus GA-induced parthenocarpy in fig is co-modulated by the female flowers and receptacle, and repression of ABA and ethylene biosynthesis and GA catabolism might be the main forces deflecting abscission and producing fig parthenocarpy.

Authors+Show Affiliations

College of Horticulture, China Agricultural University, Beijing, People's Republic of China.College of Horticulture, China Agricultural University, Beijing, People's Republic of China.College of Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People's Republic of China.Department of Fruit Tree Sciences, Agricultural Research Organization, Volcani Center, Bet-Dagan, Israel.College of Horticulture, China Agricultural University, Beijing, People's Republic of China. hqma@cau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29859043

Citation

Chai, Lijuan, et al. "Transcriptome Analysis Unravels Spatiotemporal Modulation of Phytohormone-pathway Expression Underlying Gibberellin-induced Parthenocarpic Fruit Set in San Pedro-type Fig (Ficus Carica L.)." BMC Plant Biology, vol. 18, no. 1, 2018, p. 100.
Chai L, Chai P, Chen S, et al. Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.). BMC Plant Biol. 2018;18(1):100.
Chai, L., Chai, P., Chen, S., Flaishman, M. A., & Ma, H. (2018). Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.). BMC Plant Biology, 18(1), 100. https://doi.org/10.1186/s12870-018-1318-1
Chai L, et al. Transcriptome Analysis Unravels Spatiotemporal Modulation of Phytohormone-pathway Expression Underlying Gibberellin-induced Parthenocarpic Fruit Set in San Pedro-type Fig (Ficus Carica L.). BMC Plant Biol. 2018 Jun 1;18(1):100. PubMed PMID: 29859043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptome analysis unravels spatiotemporal modulation of phytohormone-pathway expression underlying gibberellin-induced parthenocarpic fruit set in San Pedro-type fig (Ficus carica L.). AU - Chai,Lijuan, AU - Chai,Peng, AU - Chen,Shangwu, AU - Flaishman,Moshe A, AU - Ma,Huiqin, Y1 - 2018/06/01/ PY - 2017/09/09/received PY - 2018/05/24/accepted PY - 2018/6/3/entrez PY - 2018/6/3/pubmed PY - 2019/1/30/medline KW - Ficus carica KW - Gibberellin treatment KW - Parthenocarpy KW - Plant hormone KW - Transcriptome analysis SP - 100 EP - 100 JF - BMC plant biology JO - BMC Plant Biol VL - 18 IS - 1 N2 - BACKGROUND: Gibberellin (GA) treatments can induce parthenocarpy in the main crop of San Pedro-type figs, the native non-parthenocarpic fruit, however, the underlying mechanism is still largely unclear. RESULTS: In our study, GA3 was applied to San Pedro-type fig main crop at anthesis. Sharply increased GA3 content was detected in both female flowers and receptacle, along with significantly decreased indole-3-acetic acid (IAA), zeatin and abscisic acid (ABA) levels in female flowers, and increased zeatin peak intensity and earlier ABA peak in receptacles. Transcriptome comparison between control and treatment groups identified more differentially expressed genes (DEGs) in receptacles than in female flowers 2 and 4 days after treatment (DAT); 10 DAT, the number of DEGs became similar in the two tissues. Synchronized changing trends of phytohormone-associated DEGs were observed in female flowers and receptacles with fruit development. Modulation of ethylene and GA signaling and auxin metabolism by exogenous GA3 occurred mainly 2 DAT, whereas changes in auxin, cytokinin and ABA signaling occurred mainly 10 DAT. Auxin-, ethylene- and ABA-metabolism and response pathways were largely regulated in the two tissues, mostly 2 and 10 DAT. The major components altering fig phytohormone metabolic and response patterns included downregulated GA2ox, BAS1, NCED and ACO, and upregulated ABA 8'-h and AUX/IAA. CONCLUSIONS: Thus GA-induced parthenocarpy in fig is co-modulated by the female flowers and receptacle, and repression of ABA and ethylene biosynthesis and GA catabolism might be the main forces deflecting abscission and producing fig parthenocarpy. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/29859043/Transcriptome_analysis_unravels_spatiotemporal_modulation_of_phytohormone_pathway_expression_underlying_gibberellin_induced_parthenocarpic_fruit_set_in_San_Pedro_type_fig__Ficus_carica_L___ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-018-1318-1 DB - PRIME DP - Unbound Medicine ER -