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High frequency of subclinical Leishmania infection among HIV-infected patients living in the endemic areas of visceral leishmaniasis in Fars province, southern Iran.
Parasitol Res. 2018 Aug; 117(8):2591-2595.PR

Abstract

Visceral leishmaniasis (VL) is a major health concern in patients with HIV infection in endemic areas of VL. In these areas, a substantial number of infected individuals are asymptomatic and the risk of acute VL infection in HIV/VL co-infected cases is high. The current study aimed to determine the prevalence of asymptomatic VL infection among HIV-infected patients in Fars province, southern Iran. Subjects of the study were 251 HIV-confirmed patients who all were clinically asymptomatic for leishmaniasis. Blood samples were obtained from each participant. Anti-Leishmania antibodies were detected in the sera using ELISA. DNA was extracted from the buffy coat of each subject and PCR amplified, targeting an ITS-2 gene of Leishmania. PCR products were purified from the gel and were sequenced. Overall, 19 out of 251 (7.6%) HIV-infected patients were found to be infected with Leishmania, using serological or molecular methods. Anti-Leishmania antibodies were detected in 13 (5.2%) patients and leishmanial DNA in 8 (3.2%) of the patients. The sequence analysis of DNA-positive cases revealed the species of the parasite as L. infantum. The high prevalence of VL among the patients with HIV is a serious challenge which demands further attention to improve the prophylaxis and treatment measurements of VL/HIV co-infection and thereby promoting the life expectancy and quality of life of these patients.

Authors+Show Affiliations

Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. sarkarib@sums.ac.ir. Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. sarkarib@sums.ac.ir.Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29860572

Citation

Rezaei, Z, et al. "High Frequency of Subclinical Leishmania Infection Among HIV-infected Patients Living in the Endemic Areas of Visceral Leishmaniasis in Fars Province, Southern Iran." Parasitology Research, vol. 117, no. 8, 2018, pp. 2591-2595.
Rezaei Z, Sarkari B, Dehghani M, et al. High frequency of subclinical Leishmania infection among HIV-infected patients living in the endemic areas of visceral leishmaniasis in Fars province, southern Iran. Parasitol Res. 2018;117(8):2591-2595.
Rezaei, Z., Sarkari, B., Dehghani, M., Layegh Gigloo, A., & Afrashteh, M. (2018). High frequency of subclinical Leishmania infection among HIV-infected patients living in the endemic areas of visceral leishmaniasis in Fars province, southern Iran. Parasitology Research, 117(8), 2591-2595. https://doi.org/10.1007/s00436-018-5949-9
Rezaei Z, et al. High Frequency of Subclinical Leishmania Infection Among HIV-infected Patients Living in the Endemic Areas of Visceral Leishmaniasis in Fars Province, Southern Iran. Parasitol Res. 2018;117(8):2591-2595. PubMed PMID: 29860572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High frequency of subclinical Leishmania infection among HIV-infected patients living in the endemic areas of visceral leishmaniasis in Fars province, southern Iran. AU - Rezaei,Z, AU - Sarkari,B, AU - Dehghani,M, AU - Layegh Gigloo,A, AU - Afrashteh,M, Y1 - 2018/06/02/ PY - 2018/04/11/received PY - 2018/05/24/accepted PY - 2018/6/4/pubmed PY - 2018/10/26/medline PY - 2018/6/4/entrez KW - Co-infection KW - HIV KW - Iran KW - Leishmania infantum KW - Subclinical KW - Visceral leishmaniasis SP - 2591 EP - 2595 JF - Parasitology research JO - Parasitol. Res. VL - 117 IS - 8 N2 - Visceral leishmaniasis (VL) is a major health concern in patients with HIV infection in endemic areas of VL. In these areas, a substantial number of infected individuals are asymptomatic and the risk of acute VL infection in HIV/VL co-infected cases is high. The current study aimed to determine the prevalence of asymptomatic VL infection among HIV-infected patients in Fars province, southern Iran. Subjects of the study were 251 HIV-confirmed patients who all were clinically asymptomatic for leishmaniasis. Blood samples were obtained from each participant. Anti-Leishmania antibodies were detected in the sera using ELISA. DNA was extracted from the buffy coat of each subject and PCR amplified, targeting an ITS-2 gene of Leishmania. PCR products were purified from the gel and were sequenced. Overall, 19 out of 251 (7.6%) HIV-infected patients were found to be infected with Leishmania, using serological or molecular methods. Anti-Leishmania antibodies were detected in 13 (5.2%) patients and leishmanial DNA in 8 (3.2%) of the patients. The sequence analysis of DNA-positive cases revealed the species of the parasite as L. infantum. The high prevalence of VL among the patients with HIV is a serious challenge which demands further attention to improve the prophylaxis and treatment measurements of VL/HIV co-infection and thereby promoting the life expectancy and quality of life of these patients. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/29860572/High_frequency_of_subclinical_Leishmania_infection_among_HIV_infected_patients_living_in_the_endemic_areas_of_visceral_leishmaniasis_in_Fars_province_southern_Iran_ L2 - https://dx.doi.org/10.1007/s00436-018-5949-9 DB - PRIME DP - Unbound Medicine ER -