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Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens.
Proc Natl Acad Sci U S A 1985; 82(9):2669-73PN

Abstract

Analyses were made of the host-dependent-variation (hdv) locus of the virulence (vir) region of the pTiC58 plasmid of Agrobacterium tumefaciens. The hdv locus is comprised of at least four genes that encode polypeptides of 13, 15, 29, and 28 kDa. Insertion of transposon Tn5 in the first gene abolishes the expression of all four genes in vitro and in vivo. Nucleotide sequence analysis of the hdv locus revealed four open reading frames tandemly arranged with spacer sequences having no promoter-like sequences and lacking the ability to bind A. tumefaciens RNA polymerase. These studies suggest that the hdv locus is comprised of at least four genes arranged in an operon in the vir region. The protein products of these genes are likely to function in some aspect of the host-range determination of A. tumefaciens.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2986128

Citation

Hagiya, M, et al. "Identification of pTiC58 Plasmid-encoded Proteins for Virulence in Agrobacterium Tumefaciens." Proceedings of the National Academy of Sciences of the United States of America, vol. 82, no. 9, 1985, pp. 2669-73.
Hagiya M, Close TJ, Tait RC, et al. Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens. Proc Natl Acad Sci USA. 1985;82(9):2669-73.
Hagiya, M., Close, T. J., Tait, R. C., & Kado, C. I. (1985). Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens. Proceedings of the National Academy of Sciences of the United States of America, 82(9), pp. 2669-73.
Hagiya M, et al. Identification of pTiC58 Plasmid-encoded Proteins for Virulence in Agrobacterium Tumefaciens. Proc Natl Acad Sci USA. 1985;82(9):2669-73. PubMed PMID: 2986128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens. AU - Hagiya,M, AU - Close,T J, AU - Tait,R C, AU - Kado,C I, PY - 1985/5/1/pubmed PY - 1985/5/1/medline PY - 1985/5/1/entrez SP - 2669 EP - 73 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 82 IS - 9 N2 - Analyses were made of the host-dependent-variation (hdv) locus of the virulence (vir) region of the pTiC58 plasmid of Agrobacterium tumefaciens. The hdv locus is comprised of at least four genes that encode polypeptides of 13, 15, 29, and 28 kDa. Insertion of transposon Tn5 in the first gene abolishes the expression of all four genes in vitro and in vivo. Nucleotide sequence analysis of the hdv locus revealed four open reading frames tandemly arranged with spacer sequences having no promoter-like sequences and lacking the ability to bind A. tumefaciens RNA polymerase. These studies suggest that the hdv locus is comprised of at least four genes arranged in an operon in the vir region. The protein products of these genes are likely to function in some aspect of the host-range determination of A. tumefaciens. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/2986128/Identification_of_pTiC58_plasmid_encoded_proteins_for_virulence_in_Agrobacterium_tumefaciens_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=2986128 DB - PRIME DP - Unbound Medicine ER -