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Venom proteomics and antivenom neutralization for the Chinese eastern Russell's viper, Daboia siamensis from Guangxi and Taiwan.
Sci Rep. 2018 06 04; 8(1):8545.SR

Abstract

The eastern Russell's viper (Daboia siamensis) causes primarily hemotoxic envenomation. Applying shotgun proteomic approach, the present study unveiled the protein complexity and geographical variation of eastern D. siamensis venoms originated from Guangxi and Taiwan. The snake venoms from the two geographical locales shared comparable expression of major proteins notwithstanding variability in their toxin proteoforms. More than 90% of total venom proteins belong to the toxin families of Kunitz-type serine protease inhibitor, phospholipase A2, C-type lectin/lectin-like protein, serine protease and metalloproteinase. Daboia siamensis Monovalent Antivenom produced in Taiwan (DsMAV-Taiwan) was immunoreactive toward the Guangxi D. siamensis venom, and effectively neutralized the venom lethality at a potency of 1.41 mg venom per ml antivenom. This was corroborated by the antivenom effective neutralization against the venom procoagulant (ED = 0.044 ± 0.002 µl, 2.03 ± 0.12 mg/ml) and hemorrhagic (ED50 = 0.871 ± 0.159 µl, 7.85 ± 3.70 mg/ml) effects. The hetero-specific Chinese pit viper antivenoms i.e. Deinagkistrodon acutus Monovalent Antivenom and Gloydius brevicaudus Monovalent Antivenom showed negligible immunoreactivity and poor neutralization against the Guangxi D. siamensis venom. The findings suggest the need for improving treatment of D. siamensis envenomation in the region through the production and the use of appropriate antivenom.

Authors+Show Affiliations

Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. tanch@um.edu.my.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29867131

Citation

Tan, Kae Yi, et al. "Venom Proteomics and Antivenom Neutralization for the Chinese Eastern Russell's Viper, Daboia Siamensis From Guangxi and Taiwan." Scientific Reports, vol. 8, no. 1, 2018, p. 8545.
Tan KY, Tan NH, Tan CH. Venom proteomics and antivenom neutralization for the Chinese eastern Russell's viper, Daboia siamensis from Guangxi and Taiwan. Sci Rep. 2018;8(1):8545.
Tan, K. Y., Tan, N. H., & Tan, C. H. (2018). Venom proteomics and antivenom neutralization for the Chinese eastern Russell's viper, Daboia siamensis from Guangxi and Taiwan. Scientific Reports, 8(1), 8545. https://doi.org/10.1038/s41598-018-25955-y
Tan KY, Tan NH, Tan CH. Venom Proteomics and Antivenom Neutralization for the Chinese Eastern Russell's Viper, Daboia Siamensis From Guangxi and Taiwan. Sci Rep. 2018 06 4;8(1):8545. PubMed PMID: 29867131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Venom proteomics and antivenom neutralization for the Chinese eastern Russell's viper, Daboia siamensis from Guangxi and Taiwan. AU - Tan,Kae Yi, AU - Tan,Nget Hong, AU - Tan,Choo Hock, Y1 - 2018/06/04/ PY - 2017/09/27/received PY - 2018/04/06/accepted PY - 2018/6/6/entrez PY - 2018/6/6/pubmed PY - 2019/10/12/medline SP - 8545 EP - 8545 JF - Scientific reports JO - Sci Rep VL - 8 IS - 1 N2 - The eastern Russell's viper (Daboia siamensis) causes primarily hemotoxic envenomation. Applying shotgun proteomic approach, the present study unveiled the protein complexity and geographical variation of eastern D. siamensis venoms originated from Guangxi and Taiwan. The snake venoms from the two geographical locales shared comparable expression of major proteins notwithstanding variability in their toxin proteoforms. More than 90% of total venom proteins belong to the toxin families of Kunitz-type serine protease inhibitor, phospholipase A2, C-type lectin/lectin-like protein, serine protease and metalloproteinase. Daboia siamensis Monovalent Antivenom produced in Taiwan (DsMAV-Taiwan) was immunoreactive toward the Guangxi D. siamensis venom, and effectively neutralized the venom lethality at a potency of 1.41 mg venom per ml antivenom. This was corroborated by the antivenom effective neutralization against the venom procoagulant (ED = 0.044 ± 0.002 µl, 2.03 ± 0.12 mg/ml) and hemorrhagic (ED50 = 0.871 ± 0.159 µl, 7.85 ± 3.70 mg/ml) effects. The hetero-specific Chinese pit viper antivenoms i.e. Deinagkistrodon acutus Monovalent Antivenom and Gloydius brevicaudus Monovalent Antivenom showed negligible immunoreactivity and poor neutralization against the Guangxi D. siamensis venom. The findings suggest the need for improving treatment of D. siamensis envenomation in the region through the production and the use of appropriate antivenom. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/29867131/Venom_proteomics_and_antivenom_neutralization_for_the_Chinese_eastern_Russell's_viper_Daboia_siamensis_from_Guangxi_and_Taiwan_ L2 - https://doi.org/10.1038/s41598-018-25955-y DB - PRIME DP - Unbound Medicine ER -