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Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines.
J Assoc Res Otolaryngol 2018; 19(4):381-399JA

Abstract

Utricles are vestibular sense organs that encode linear head movements. They are composed of a sensory epithelium with type I and type II hair cells and supporting cells, sitting atop connective tissue, through which vestibular nerves project. We characterized utricular Cre expression in 11 murine CreER lines using the ROSA26tdTomato reporter line and tamoxifen induction at 6 weeks of age. This characterization included Calbindin2CreERT2, Fgfr3-iCreERT2, GFAP-A-CreER™, GFAP-B-CreER™, GLAST-CreERT2, Id2CreERT2, OtoferlinCreERT2, ParvalbuminCreERT2, Prox1CreERT2, Sox2CreERT2, and Sox9-CreERT2. OtoferlinCreERT2 mice had inducible Cre activity specific to hair cells. GLAST-CreERT2, Id2CreERT2, and Sox9-CreERT2 had inducible Cre activity specific to supporting cells. Sox2CreERT2 had inducible Cre activity in supporting cells and most type II hair cells. ParvalbuminCreERT2 mice had small numbers of labeled vestibular nerve afferents. Calbindin2CreERT2 mice had labeling of most type II hair cells and some type I hair cells and supporting cells. Only rare (or no) tdTomato-positive cells were detected in utricles of Fgfr3-iCreERT2, GFAP-A-CreER™, GFAP-B-CreER™, and Prox1CreERT2 mice. No Cre leakiness (tdTomato expression in the absence of tamoxifen) was observed in OtoferlinCreERT2 mice. A small degree of leakiness was seen in GLAST-CreERT2, Id2CreERT2, Sox2CreERT2, and Sox9-CreERT2 lines. Calbindin2CreERT2 mice had similar tdTomato expression with or without tamoxifen, indicating lack of inducible control under the conditions tested. In conclusion, 5 lines-GLAST-CreERT2, Id2CreERT2, OtoferlinCreERT2, Sox2CreERT2, and Sox9-CreERT2-showed cell-selective, inducible Cre activity with little leakiness, providing new genetic tools for researchers studying the vestibular periphery.

Authors+Show Affiliations

Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, WA, USA.Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, WA, USA.Department of Otolaryngology-Head and Neck Surgery, Virginia Merrill Bloedel Hearing Research Center, University of Washington, Seattle, WA, USA.Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN, USA.Departments of Pharmacology and Surgery, Division of Otolaryngology, Southern Illinois University School of Medicine, Springfield, IL, USA. bcox@siumed.edu.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

29869046

Citation

Stone, Jennifer S., et al. "Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines." Journal of the Association for Research in Otolaryngology : JARO, vol. 19, no. 4, 2018, pp. 381-399.
Stone JS, Wisner SR, Bucks SA, et al. Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines. J Assoc Res Otolaryngol. 2018;19(4):381-399.
Stone, J. S., Wisner, S. R., Bucks, S. A., Mellado Lagarde, M. M., & Cox, B. C. (2018). Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines. Journal of the Association for Research in Otolaryngology : JARO, 19(4), pp. 381-399. doi:10.1007/s10162-018-0676-6.
Stone JS, et al. Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines. J Assoc Res Otolaryngol. 2018;19(4):381-399. PubMed PMID: 29869046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Adult Vestibular Organs in 11 CreER Mouse Lines. AU - Stone,Jennifer S, AU - Wisner,Serena R, AU - Bucks,Stephanie A, AU - Mellado Lagarde,Marcia M, AU - Cox,Brandon C, Y1 - 2018/06/04/ PY - 2017/12/19/received PY - 2018/05/07/accepted PY - 2018/6/6/pubmed PY - 2019/7/6/medline PY - 2018/6/6/entrez KW - CreER/loxP KW - FGFR3 KW - GFAP KW - GLAST KW - Id2 KW - Prox1 KW - Sox2 KW - Sox9 KW - calbindin KW - fate-mapping KW - mouse genetics KW - otoferlin KW - parvalbumin KW - utricle SP - 381 EP - 399 JF - Journal of the Association for Research in Otolaryngology : JARO JO - J. Assoc. Res. Otolaryngol. VL - 19 IS - 4 N2 - Utricles are vestibular sense organs that encode linear head movements. They are composed of a sensory epithelium with type I and type II hair cells and supporting cells, sitting atop connective tissue, through which vestibular nerves project. We characterized utricular Cre expression in 11 murine CreER lines using the ROSA26tdTomato reporter line and tamoxifen induction at 6 weeks of age. This characterization included Calbindin2CreERT2, Fgfr3-iCreERT2, GFAP-A-CreER™, GFAP-B-CreER™, GLAST-CreERT2, Id2CreERT2, OtoferlinCreERT2, ParvalbuminCreERT2, Prox1CreERT2, Sox2CreERT2, and Sox9-CreERT2. OtoferlinCreERT2 mice had inducible Cre activity specific to hair cells. GLAST-CreERT2, Id2CreERT2, and Sox9-CreERT2 had inducible Cre activity specific to supporting cells. Sox2CreERT2 had inducible Cre activity in supporting cells and most type II hair cells. ParvalbuminCreERT2 mice had small numbers of labeled vestibular nerve afferents. Calbindin2CreERT2 mice had labeling of most type II hair cells and some type I hair cells and supporting cells. Only rare (or no) tdTomato-positive cells were detected in utricles of Fgfr3-iCreERT2, GFAP-A-CreER™, GFAP-B-CreER™, and Prox1CreERT2 mice. No Cre leakiness (tdTomato expression in the absence of tamoxifen) was observed in OtoferlinCreERT2 mice. A small degree of leakiness was seen in GLAST-CreERT2, Id2CreERT2, Sox2CreERT2, and Sox9-CreERT2 lines. Calbindin2CreERT2 mice had similar tdTomato expression with or without tamoxifen, indicating lack of inducible control under the conditions tested. In conclusion, 5 lines-GLAST-CreERT2, Id2CreERT2, OtoferlinCreERT2, Sox2CreERT2, and Sox9-CreERT2-showed cell-selective, inducible Cre activity with little leakiness, providing new genetic tools for researchers studying the vestibular periphery. SN - 1438-7573 UR - https://www.unboundmedicine.com/medline/citation/29869046/Characterization_of_Adult_Vestibular_Organs_in_11_CreER_Mouse_Lines_ L2 - https://dx.doi.org/10.1007/s10162-018-0676-6 DB - PRIME DP - Unbound Medicine ER -