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Polyphenolic profiling of Ipomoea carnea Jacq. by HPLC-DAD and its implications in oxidative stress and cancer.
Nat Prod Res. 2019 Jul; 33(14):2099-2104.NP

Abstract

Ipomoea carnea Jacq. is an important folklore medicinal plant, assessed for its underexplored biological potential. Antioxidant, cytotoxic, antiproliferative and polyphenolic profile of whole plant was evaluated using various techniques. Maximum extract recovery (29% w/w), phenolic [13.54 ± 0.27 μg GAE/mg dry weight (DW)] and flavonoid (2.11 ± 0.10 μg QE /mg DW) content were recorded in methanol-distilled water (1:1) flower extract. HPLC-DAD analysis quantified substantial amount of six different polyphenols ranging from 0.081 to 37.95 μg/mg extract. Maximum total antioxidant and reducing potential were documented in methanol-distilled water and acetone-distilled water flower extracts (42.62 ± 0.47 and 24.38 ± 0.39 μg AAE/mg DW) respectively. Ethanol-chloroform root extract manifested highest free radical scavenging (IC50 of 61.22 μg/mL) while 94.64% of the extracts showed cytotoxicity against brine shrimps. Ethanol leaf extract exhibited remarkable activity against THP-1 cell line (IC50 = 8 ± 0.05 μg/mL) and protein kinases (31 mm phenotype bald zone).

Authors+Show Affiliations

a Faculty of Biological Sciences, Department of Pharmacy , Quaid-i-Azam University , Islamabad , Pakistan.a Faculty of Biological Sciences, Department of Pharmacy , Quaid-i-Azam University , Islamabad , Pakistan.a Faculty of Biological Sciences, Department of Pharmacy , Quaid-i-Azam University , Islamabad , Pakistan.a Faculty of Biological Sciences, Department of Pharmacy , Quaid-i-Azam University , Islamabad , Pakistan.b Faculty of Biological Sciences, Department of Biochemistry , Quaid-i-Azam University , Islamabad , Pakistan.a Faculty of Biological Sciences, Department of Pharmacy , Quaid-i-Azam University , Islamabad , Pakistan.c Centre for Advanced Drug Research , COMSATS Institute of Information Technology , Abbottabad , Pakistan. d School of Pharmacy , Monash University, Jalan Lagoon Selatan , Subang Jaya , Malaysia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29873254

Citation

Khan, Muhammad Zafar Irshad, et al. "Polyphenolic Profiling of Ipomoea Carnea Jacq. By HPLC-DAD and Its Implications in Oxidative Stress and Cancer." Natural Product Research, vol. 33, no. 14, 2019, pp. 2099-2104.
Khan MZI, Zahra SS, Ahmed M, et al. Polyphenolic profiling of Ipomoea carnea Jacq. by HPLC-DAD and its implications in oxidative stress and cancer. Nat Prod Res. 2019;33(14):2099-2104.
Khan, M. Z. I., Zahra, S. S., Ahmed, M., Fatima, H., Mirza, B., Haq, I. U., & Khan, S. U. (2019). Polyphenolic profiling of Ipomoea carnea Jacq. by HPLC-DAD and its implications in oxidative stress and cancer. Natural Product Research, 33(14), 2099-2104. https://doi.org/10.1080/14786419.2018.1482551
Khan MZI, et al. Polyphenolic Profiling of Ipomoea Carnea Jacq. By HPLC-DAD and Its Implications in Oxidative Stress and Cancer. Nat Prod Res. 2019;33(14):2099-2104. PubMed PMID: 29873254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenolic profiling of Ipomoea carnea Jacq. by HPLC-DAD and its implications in oxidative stress and cancer. AU - Khan,Muhammad Zafar Irshad, AU - Zahra,Syeda Saniya, AU - Ahmed,Madiha, AU - Fatima,Humaira, AU - Mirza,Bushra, AU - Haq,Ihsan-Ul, AU - Khan,Shafi Ullah, Y1 - 2018/06/06/ PY - 2018/6/7/pubmed PY - 2019/8/24/medline PY - 2018/6/7/entrez KW - KW - THP-1 leukemia cell line KW - antimicrobial KW - antioxidant KW - cytotoxicity KW - protein kinase inhibition SP - 2099 EP - 2104 JF - Natural product research JO - Nat Prod Res VL - 33 IS - 14 N2 - Ipomoea carnea Jacq. is an important folklore medicinal plant, assessed for its underexplored biological potential. Antioxidant, cytotoxic, antiproliferative and polyphenolic profile of whole plant was evaluated using various techniques. Maximum extract recovery (29% w/w), phenolic [13.54 ± 0.27 μg GAE/mg dry weight (DW)] and flavonoid (2.11 ± 0.10 μg QE /mg DW) content were recorded in methanol-distilled water (1:1) flower extract. HPLC-DAD analysis quantified substantial amount of six different polyphenols ranging from 0.081 to 37.95 μg/mg extract. Maximum total antioxidant and reducing potential were documented in methanol-distilled water and acetone-distilled water flower extracts (42.62 ± 0.47 and 24.38 ± 0.39 μg AAE/mg DW) respectively. Ethanol-chloroform root extract manifested highest free radical scavenging (IC50 of 61.22 μg/mL) while 94.64% of the extracts showed cytotoxicity against brine shrimps. Ethanol leaf extract exhibited remarkable activity against THP-1 cell line (IC50 = 8 ± 0.05 μg/mL) and protein kinases (31 mm phenotype bald zone). SN - 1478-6427 UR - https://www.unboundmedicine.com/medline/citation/29873254/Polyphenolic_profiling_of_Ipomoea_carnea_Jacq__by_HPLC_DAD_and_its_implications_in_oxidative_stress_and_cancer_ L2 - https://www.tandfonline.com/doi/full/10.1080/14786419.2018.1482551 DB - PRIME DP - Unbound Medicine ER -