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Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean).
FEMS Microbiol Ecol. 2018 08 01; 94(8)FM

Abstract

The saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) is dominated by the bloom-forming cyanobacterium Arthrospira. However, the rest of the phototrophic community remains underexplored because of their minute dimension or lower biomass. To characterize the phototrophic microorganisms living in this ecosystem considered as a modern analog of Precambrian environments, several strains were isolated from the water column and stromatolites and analyzed using the polyphasic approach. Based on morphological, ultrastructural and molecular (16S rRNA gene, 18S rRNA gene, 16S-23S internal transcribed spacer (ITS) region and cpcBA-IGS locus) methods, seven filamentous cyanobacteria and the prasinophyte Picocystis salinarum were identified. Two new genera and four new cyanobacteria species belonging to the orders Oscillatoriales (Desertifilum dzianense sp. nov.) and Synechococcales (Sodalinema komarekii gen. nov., sp. nov., Sodaleptolyngbya stromatolitii gen. nov., sp. nov. and Haloleptolyngbya elongata sp. nov.) were described. This approach also allowed to identify Arthrospira fusiformis with exclusively straight trichomes instead of the spirally coiled form commonly observed in the genus. This study evidenced the importance of using the polyphasic approach to solve the complex taxonomy of cyanobacteria and to study algal assemblages from unexplored ecosystems.

Authors+Show Affiliations

UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France. Phyto-Quality, 15 rue Pétrarque, 75116 Paris, France.UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France.UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France.UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France. Electron Microscopy Platform, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France.UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France. Electron Microscopy Platform, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France.UMR 7266 LIENSs CNRS, Université de La Rochelle, F-17000 La Rochelle, France.UMR 9190 MARBEC IRD-CNRS, Université de Montpellier-IFREMER, Avenue Jean Monnet, F-34200 Sète, France.Institut de Physique du Globe de Paris-Sorbonne Paris Cité, Université Paris Diderot, UMR 7154 CNRS, F-75005 Paris, France.UMR 7245 MCAM MNHN-CNRS, Muséum National d'Histoire Naturelle, CP 39, 12 rue Buffon, F-75231 Paris Cedex 05, France.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29878107

Citation

Cellamare, M, et al. "Characterization of Phototrophic Microorganisms and Description of New Cyanobacteria Isolated From the Saline-alkaline Crater-lake Dziani Dzaha (Mayotte, Indian Ocean)." FEMS Microbiology Ecology, vol. 94, no. 8, 2018.
Cellamare M, Duval C, Drelin Y, et al. Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean). FEMS Microbiol Ecol. 2018;94(8).
Cellamare, M., Duval, C., Drelin, Y., Djediat, C., Touibi, N., Agogué, H., Leboulanger, C., Ader, M., & Bernard, C. (2018). Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean). FEMS Microbiology Ecology, 94(8). https://doi.org/10.1093/femsec/fiy108
Cellamare M, et al. Characterization of Phototrophic Microorganisms and Description of New Cyanobacteria Isolated From the Saline-alkaline Crater-lake Dziani Dzaha (Mayotte, Indian Ocean). FEMS Microbiol Ecol. 2018 08 1;94(8) PubMed PMID: 29878107.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean). AU - Cellamare,M, AU - Duval,C, AU - Drelin,Y, AU - Djediat,C, AU - Touibi,N, AU - Agogué,H, AU - Leboulanger,C, AU - Ader,M, AU - Bernard,C, PY - 2018/01/11/received PY - 2018/06/03/accepted PY - 2018/6/8/pubmed PY - 2019/5/17/medline PY - 2018/6/8/entrez JF - FEMS microbiology ecology JO - FEMS Microbiol Ecol VL - 94 IS - 8 N2 - The saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) is dominated by the bloom-forming cyanobacterium Arthrospira. However, the rest of the phototrophic community remains underexplored because of their minute dimension or lower biomass. To characterize the phototrophic microorganisms living in this ecosystem considered as a modern analog of Precambrian environments, several strains were isolated from the water column and stromatolites and analyzed using the polyphasic approach. Based on morphological, ultrastructural and molecular (16S rRNA gene, 18S rRNA gene, 16S-23S internal transcribed spacer (ITS) region and cpcBA-IGS locus) methods, seven filamentous cyanobacteria and the prasinophyte Picocystis salinarum were identified. Two new genera and four new cyanobacteria species belonging to the orders Oscillatoriales (Desertifilum dzianense sp. nov.) and Synechococcales (Sodalinema komarekii gen. nov., sp. nov., Sodaleptolyngbya stromatolitii gen. nov., sp. nov. and Haloleptolyngbya elongata sp. nov.) were described. This approach also allowed to identify Arthrospira fusiformis with exclusively straight trichomes instead of the spirally coiled form commonly observed in the genus. This study evidenced the importance of using the polyphasic approach to solve the complex taxonomy of cyanobacteria and to study algal assemblages from unexplored ecosystems. SN - 1574-6941 UR - https://www.unboundmedicine.com/medline/citation/29878107/Characterization_of_phototrophic_microorganisms_and_description_of_new_cyanobacteria_isolated_from_the_saline_alkaline_crater_lake_Dziani_Dzaha__Mayotte_Indian_Ocean__ L2 - https://academic.oup.com/femsec/article-lookup/doi/10.1093/femsec/fiy108 DB - PRIME DP - Unbound Medicine ER -