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Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria.
Toxins (Basel). 2018 06 07; 10(6)T

Abstract

The venom of the spider Heteropoda venatoria produced lethal effect to cockroaches as reported in our previous study, and could be a resource for naturally-occurring insecticides. The present study characterized a novel cockroach voltage-gated sodium channels (NaVs) antagonist, μ-sparatoxin-Hv2 (μ-SPRTX-Hv2 for short), from this venom. μ-SPRTX-Hv2 is composed of 37 amino acids and contains six conserved cysteines. We synthesized the toxin by using the chemical synthesis method. The toxin was lethal to cockroaches when intraperitoneally injected, with a LD50 value of 2.8 nmol/g of body weight. Electrophysiological data showed that the toxin potently blocked NaVs in cockroach dorsal unpaired median (DUM) neurons, with an IC50 of 833.7 ± 132.2 nM, but it hardly affected the DUM voltage-gated potassium channels (KVs) and the DUM high-voltage-activated calcium channels (HVA CaVs). The toxin also did not affect NaVs, HVA CaVs, and Kvs in rat dorsal root ganglion (DRG) neurons, as well as NaV subtypes NaV1.3⁻1.5, NaV1.7, and NaV1.8. No envenomation symptoms were observed when μ-SPRTX-Hv2 was intraperitoneally injected into mouse at the dose of 7.0 μg/g. In summary, μ-SPRTX-Hv2 is a novel insecticidal toxin from H. venatoria venom. It might exhibit its effect by blocking the insect NaVs and is a candidate for developing bioinsecticide.

Authors+Show Affiliations

The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. Zhen-Xiao@outlook.com.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. Zhangyx@smail.hunnu.edu.cn.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. Jiaozeng_@outlook.com.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. liangsp@hunnu.edu.cn.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. chengtang@hunnu.edu.cn.The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha 410081, China. liuzh@hunnu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

29880771

Citation

Xiao, Zhen, et al. "Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, From the Venom of the Spider Heteropoda Venatoria." Toxins, vol. 10, no. 6, 2018.
Xiao Z, Zhang Y, Zeng J, et al. Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria. Toxins (Basel). 2018;10(6).
Xiao, Z., Zhang, Y., Zeng, J., Liang, S., Tang, C., & Liu, Z. (2018). Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria. Toxins, 10(6). https://doi.org/10.3390/toxins10060233
Xiao Z, et al. Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, From the Venom of the Spider Heteropoda Venatoria. Toxins (Basel). 2018 06 7;10(6) PubMed PMID: 29880771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification and Characterization of a Novel Insecticidal Toxin, μ-sparatoxin-Hv2, from the Venom of the Spider Heteropoda venatoria. AU - Xiao,Zhen, AU - Zhang,Yunxiao, AU - Zeng,Jiao, AU - Liang,Songping, AU - Tang,Cheng, AU - Liu,Zhonghua, Y1 - 2018/06/07/ PY - 2018/05/18/received PY - 2018/06/03/revised PY - 2018/06/06/accepted PY - 2018/6/9/entrez PY - 2018/6/9/pubmed PY - 2019/4/6/medline KW - bioinsecticide KW - spider peptide toxin KW - voltage-gated sodium channels JF - Toxins JO - Toxins (Basel) VL - 10 IS - 6 N2 - The venom of the spider Heteropoda venatoria produced lethal effect to cockroaches as reported in our previous study, and could be a resource for naturally-occurring insecticides. The present study characterized a novel cockroach voltage-gated sodium channels (NaVs) antagonist, μ-sparatoxin-Hv2 (μ-SPRTX-Hv2 for short), from this venom. μ-SPRTX-Hv2 is composed of 37 amino acids and contains six conserved cysteines. We synthesized the toxin by using the chemical synthesis method. The toxin was lethal to cockroaches when intraperitoneally injected, with a LD50 value of 2.8 nmol/g of body weight. Electrophysiological data showed that the toxin potently blocked NaVs in cockroach dorsal unpaired median (DUM) neurons, with an IC50 of 833.7 ± 132.2 nM, but it hardly affected the DUM voltage-gated potassium channels (KVs) and the DUM high-voltage-activated calcium channels (HVA CaVs). The toxin also did not affect NaVs, HVA CaVs, and Kvs in rat dorsal root ganglion (DRG) neurons, as well as NaV subtypes NaV1.3⁻1.5, NaV1.7, and NaV1.8. No envenomation symptoms were observed when μ-SPRTX-Hv2 was intraperitoneally injected into mouse at the dose of 7.0 μg/g. In summary, μ-SPRTX-Hv2 is a novel insecticidal toxin from H. venatoria venom. It might exhibit its effect by blocking the insect NaVs and is a candidate for developing bioinsecticide. SN - 2072-6651 UR - https://www.unboundmedicine.com/medline/citation/29880771/Purification_and_Characterization_of_a_Novel_Insecticidal_Toxin_μ_sparatoxin_Hv2_from_the_Venom_of_the_Spider_Heteropoda_venatoria_ L2 - http://www.mdpi.com/resolver?pii=toxins10060233 DB - PRIME DP - Unbound Medicine ER -