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Phyto-mediated synthesis of silver nanoparticles using fucoidan isolated from Spatoglossum asperum and assessment of antibacterial activities.
J Photochem Photobiol B. 2018 Aug; 185:117-125.JP

Abstract

The present study was aimed to investigate the antibacterial efficacy of fucoidan mediated silver nanoparticles (Fu-AgNPs) synthesized from Spatoglossum asperum. The synthesized Fu-AgNPs were characterized by UV-visible, Field emission - scanning electron microscope (FE-SEM), Tranmission electron microscope (TEM), X-ray diffraction (XRD), Selected area electron diffraction (SAED) pattern, Energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FT-IR), Dynamic light scattering (DLS) and Zeta potential analysis. The UV-visible spectrum of Fu-AgNPs exhibited a characteristic surface plasmon resonance (SPR) peak at 440 nm. The electron microscopic results revealed that the nanoparticles were spherical to oval in shape and are found to be 20 to 46 nm. Altogether the X-ray diffraction analysis showed that the Fu-AgNPs were crystalline in nature. The FT-IR spectrum confirmed the existence of CC stretching vibration of aromatic compounds and sulfated groups of fucoidan plays a major role in the synthesis of Fu-AgNPs. The biosynthesized Fu-AgNPs shows potential antibacterial activity against Klebsiella pneumoniae in agar bioassay, disk diffusion, reactive oxygen species, protein leakage and confocal laser scanning microscopy assays. Furthermore, Artemia toxicity assay results showed less mortality (3.3 ± 0.8%) even at higher concentration of Fu-AgNPs. Therefore, Fu-AgNPs can be effectively used as an antibacterial agent in the pharmaceutical fields.

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Authors+Show Affiliations

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Biomaterials and Biotechnology in Animal Health Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Food Chemistry and Molecular Cancer Biology Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India.

Department of Bioinformatics, Science Campus, Alagappa University, Karaikudi 630 003, India.

Disease Control and Prevention Lab, Department of Animal Health and Management, Science Campus, Alagappa University, Karaikudi 630 003, India. Electronic address: 71gnmprabhu@gmail.com.

MeSH

AnimalsAnti-Bacterial AgentsArtemiaDisk Diffusion Antimicrobial TestsGreen Chemistry TechnologyKlebsiella pneumoniaeLightMetal NanoparticlesMicroscopy, ConfocalPhaeophytaPlant ExtractsPolysaccharidesReactive Oxygen SpeciesSilverSpectrometry, X-Ray EmissionSpectroscopy, Fourier Transform InfraredX-Ray Diffraction

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29886330

Citation

Ravichandran, Anjali, et al. "Phyto-mediated Synthesis of Silver Nanoparticles Using Fucoidan Isolated From Spatoglossum Asperum and Assessment of Antibacterial Activities." Journal of Photochemistry and Photobiology. B, Biology, vol. 185, 2018, pp. 117-125.

Ravichandran A, Subramanian P, Manoharan V, et al. Phyto-mediated synthesis of silver nanoparticles using fucoidan isolated from Spatoglossum asperum and assessment of antibacterial activities. J Photochem Photobiol B. 2018;185:117-125.

Ravichandran, A., Subramanian, P., Manoharan, V., Muthu, T., Periyannan, R., Thangapandi, M., Ponnuchamy, K., Pandi, B., & Marimuthu, P. N. (2018). Phyto-mediated synthesis of silver nanoparticles using fucoidan isolated from Spatoglossum asperum and assessment of antibacterial activities. Journal of Photochemistry and Photobiology. B, Biology, 185, 117-125. https://doi.org/10.1016/j.jphotobiol.2018.05.031

Ravichandran A, et al. Phyto-mediated Synthesis of Silver Nanoparticles Using Fucoidan Isolated From Spatoglossum Asperum and Assessment of Antibacterial Activities. J Photochem Photobiol B. 2018;185:117-125. PubMed PMID: 29886330.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Phyto-mediated synthesis of silver nanoparticles using fucoidan isolated from Spatoglossum asperum and assessment of antibacterial activities. AU - Ravichandran,Anjali, AU - Subramanian,Palanisamy, AU - Manoharan,Vinosha, AU - Muthu,Thenmozhi, AU - Periyannan,Rajasekar, AU - Thangapandi,Marudhupandi, AU - Ponnuchamy,Kumar, AU - Pandi,Boomi, AU - Marimuthu,Prabhu Narayanasamy, Y1 - 2018/05/31/ PY - 2018/03/13/received PY - 2018/05/24/revised PY - 2018/05/30/accepted PY - 2018/6/11/pubmed PY - 2018/7/20/medline PY - 2018/6/11/entrez KW - Antibacterial activity KW - Artemia toxicity KW - Confocal laser scanning microscopy KW - Fucoidan KW - Silver nanoparticles SP - 117 EP - 125 JF - Journal of photochemistry and photobiology. B, Biology JO - J Photochem Photobiol B VL - 185 N2 - The present study was aimed to investigate the antibacterial efficacy of fucoidan mediated silver nanoparticles (Fu-AgNPs) synthesized from Spatoglossum asperum. The synthesized Fu-AgNPs were characterized by UV-visible, Field emission - scanning electron microscope (FE-SEM), Tranmission electron microscope (TEM), X-ray diffraction (XRD), Selected area electron diffraction (SAED) pattern, Energy-dispersive X-ray spectroscopy (EDAX), Fourier transform infrared spectroscopy (FT-IR), Dynamic light scattering (DLS) and Zeta potential analysis. The UV-visible spectrum of Fu-AgNPs exhibited a characteristic surface plasmon resonance (SPR) peak at 440 nm. The electron microscopic results revealed that the nanoparticles were spherical to oval in shape and are found to be 20 to 46 nm. Altogether the X-ray diffraction analysis showed that the Fu-AgNPs were crystalline in nature. The FT-IR spectrum confirmed the existence of CC stretching vibration of aromatic compounds and sulfated groups of fucoidan plays a major role in the synthesis of Fu-AgNPs. The biosynthesized Fu-AgNPs shows potential antibacterial activity against Klebsiella pneumoniae in agar bioassay, disk diffusion, reactive oxygen species, protein leakage and confocal laser scanning microscopy assays. Furthermore, Artemia toxicity assay results showed less mortality (3.3 ± 0.8%) even at higher concentration of Fu-AgNPs. Therefore, Fu-AgNPs can be effectively used as an antibacterial agent in the pharmaceutical fields. SN - 1873-2682 UR - https://www.unboundmedicine.com/medline/citation/29886330/Phyto_mediated_synthesis_of_silver_nanoparticles_using_fucoidan_isolated_from_Spatoglossum_asperum_and_assessment_of_antibacterial_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1011-1344(18)30279-3 DB - PRIME DP - Unbound Medicine ER -