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[Acupuncture Alleviated Depressive-like Behavior by Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats].
Zhen Ci Yan Jiu. 2018 Apr 25; 43(4):226-30.ZC

Abstract

OBJECTIVE

To observe the effect of acupuncture intervention on depressive-like behavior and nuclear factor-κB (NF-κB), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in the prefrontal cortex in chronic stress induced depression rats, so as to explore its mechanism underlying antidepressant effect.

METHODS

SD rats were randomly divided into control, model, acupuncture and medication (Fluoxetine) groups (8 rats/group). The depression model was established by chro-nic unpredictable stress stimulation (fasting, water deprivation, cold water swimming, tail clamping, constraining, etc., and solitary raising) for 28 days. Acupuncture treatment was applied to "Baihui" (GV 20) and unilateral "Neiguan"(PC 6) once every other day for 14 days. Fluoxetine (10 mg/kg) was given to rats of the medication group by gavage (p.o.) before every stress stimulation, once every day for 28 days. The animals' behavior was tested by using sucrose intake and open field tests (number of crossings and rearings in 5 min). The expression of NF-κB in the prefrontal cortex was detected by Western blot, and the contents of prefrontal iNOS and NO were detected by ELISA and nitric reductases, respectively.

RESULTS

After modeling, the sucrose intake and the numbers of crossing and rearing times were significantly decreased (P<0.01), and the expression of NF-κB protein and the contents of iNOS and NO in the prefrontal cortex were significantly increased in the model group compared with the control group (P<0.01). Following the treatment, the sucrose intake and the numbers of crossing and rearing times were significantly increased in both acupuncture and medication groups compared with the model group (P<0.05, P<0.01), and the expression level of NF-κB, and iNOS and NO contents in the prefrontal cortex were significantly lower in both acupuncture and medication groups than in the model group (P<0.01). There were no significant differences between the acupuncture and medication groups in the crossing number and rearing times, NF-κB expression, and iNOS and NO levels (P>0.05).

CONCLUSION

Acupuncture treatment can improve the depressive-like behavior of depression rats, which is closely related to its effects in down-regulating the levels of NF-κB protein, iNOS and NO in the prefrontal cortex to reduce brain inflammatory damage.

Authors+Show Affiliations

School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.Guo Yitang, Hebei Cangzhou Central Hospital, Cangzhou 061000, Hebei Province.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.Department of Acupuncture and Moxibustion, Beijing Fengtai District Puhuangyu Community Health Center, Beijing 100079.School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29888575

Citation

Pu, Rong, et al. "[Acupuncture Alleviated Depressive-like Behavior By Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 4, 2018, pp. 226-30.
Pu R, Shao RH, Lu J, et al. [Acupuncture Alleviated Depressive-like Behavior by Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats]. Zhen Ci Yan Jiu. 2018;43(4):226-30.
Pu, R., Shao, R. H., Lu, J., Jin, S. Y., & Tu, Y. (2018). [Acupuncture Alleviated Depressive-like Behavior by Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(4), 226-30. https://doi.org/10.13702/j.1000-0607.170569
Pu R, et al. [Acupuncture Alleviated Depressive-like Behavior By Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats]. Zhen Ci Yan Jiu. 2018 Apr 25;43(4):226-30. PubMed PMID: 29888575.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Acupuncture Alleviated Depressive-like Behavior by Down-regulating NF-κB, iNOS and NO Levels in Prefrontal Cortex of Depression Rats]. AU - Pu,Rong, AU - Shao,Run-Hui, AU - Lu,Jun, AU - Jin,Shu-Ying, AU - Tu,Ya, PY - 2018/6/12/entrez PY - 2018/6/12/pubmed PY - 2019/4/4/medline KW - Acupuncture KW - Chronic stress KW - Depression KW - Inducible nitric oxide synthase (iNOS) KW - Nitric oxide (NO) KW - Nuclear factor-κB (NF-κB) SP - 226 EP - 30 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 4 N2 - OBJECTIVE: To observe the effect of acupuncture intervention on depressive-like behavior and nuclear factor-κB (NF-κB), inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in the prefrontal cortex in chronic stress induced depression rats, so as to explore its mechanism underlying antidepressant effect. METHODS: SD rats were randomly divided into control, model, acupuncture and medication (Fluoxetine) groups (8 rats/group). The depression model was established by chro-nic unpredictable stress stimulation (fasting, water deprivation, cold water swimming, tail clamping, constraining, etc., and solitary raising) for 28 days. Acupuncture treatment was applied to "Baihui" (GV 20) and unilateral "Neiguan"(PC 6) once every other day for 14 days. Fluoxetine (10 mg/kg) was given to rats of the medication group by gavage (p.o.) before every stress stimulation, once every day for 28 days. The animals' behavior was tested by using sucrose intake and open field tests (number of crossings and rearings in 5 min). The expression of NF-κB in the prefrontal cortex was detected by Western blot, and the contents of prefrontal iNOS and NO were detected by ELISA and nitric reductases, respectively. RESULTS: After modeling, the sucrose intake and the numbers of crossing and rearing times were significantly decreased (P<0.01), and the expression of NF-κB protein and the contents of iNOS and NO in the prefrontal cortex were significantly increased in the model group compared with the control group (P<0.01). Following the treatment, the sucrose intake and the numbers of crossing and rearing times were significantly increased in both acupuncture and medication groups compared with the model group (P<0.05, P<0.01), and the expression level of NF-κB, and iNOS and NO contents in the prefrontal cortex were significantly lower in both acupuncture and medication groups than in the model group (P<0.01). There were no significant differences between the acupuncture and medication groups in the crossing number and rearing times, NF-κB expression, and iNOS and NO levels (P>0.05). CONCLUSION: Acupuncture treatment can improve the depressive-like behavior of depression rats, which is closely related to its effects in down-regulating the levels of NF-κB protein, iNOS and NO in the prefrontal cortex to reduce brain inflammatory damage. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29888575/[Acupuncture_Alleviated_Depressive_like_Behavior_by_Down_regulating_NF_κB_iNOS_and_NO_Levels_in_Prefrontal_Cortex_of_Depression_Rats]_ L2 - https://medlineplus.gov/depression.html DB - PRIME DP - Unbound Medicine ER -