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[Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly by Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats].
Zhen Ci Yan Jiu 2018; 43(4):231-5ZC

Abstract

OBJECTIVE

To observe the effect of acupoint injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) on nasal allergic reactions and the expression of histamine receptor H 1 and H 4 in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improvement of AR.

METHODS

SD rats were randomized into normal, model, non-acupoint injection and acupoint injection groups (n=8 in each). The AR model was established by intraperitoneal (i.p.) injection of mixture of ovalbumin, aluminium hydroxide gel and normal saline (once every other day, for 7 times), and nasal drip of ovalbumin (on the following day of i.p.for 7 days). The mixture solution of lidocaine, dexamethasone (DXM) and transfer factor (0.1 mL/acupoint or non-acupoint) was injected into bilateral LI 20 and GV 29 in the acupoint injection group, or into the non-acupoints (about 5 cm below the armpit on both sides, and the middle point between the left "Houhai"[GV 1] and "Huantiao"[GB 30]), on the 1st , 5th, 9th, and 13th day after modeling. Symptoms of sneezing, nasal discharge, nose-rubbing, etc. were scored after the treatment. The expression levels of H1 R and H4 R proteins and genes in the nasal mucosa tissue were determined using immunohistochemistry and quantitative real-time PCR, respectively.

RESULTS

Compared with the normal group, the symptom score and the expression levels of H1 R, H4 R proteins and genes in nasal mucosa were significantly increased in the model group (P<0.05). After acupoint injection for 4 times, the symptom score and the expression levels of H1 R, H4 R proteins and mRNAs were significantly down-regulated in the acupoint injection group relevant to the model group (P<0.05). There were no significant differences between the non-acupoint and the model groups in the abovementioned indexes (P>0.05), suggesting a specificity of the effect of acupoint injection.

CONCLUSION

Acupoint injection can relieve the allergic symptoms of AR rats, which may be related to its effects in down-regulating the over expression of H1 R and H4 R proteins and genes in the nasal mucosa.

Authors+Show Affiliations

Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.the Affliated Hospital, Guizhou Medical University, Guiyang 550004, China.Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.the Affliated Hospital, Guizhou Medical University, Guiyang 550004, China.Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.the Affliated Hospital, Guizhou Medical University, Guiyang 550004, China.Clinical Medical College, Guizhou Medical University, Guiyang 550004, China.

Pub Type(s)

Journal Article

Language

chi

PubMed ID

29888576

Citation

Liang, Fei-Hong, et al. "[Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly By Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats]." Zhen Ci Yan Jiu = Acupuncture Research, vol. 43, no. 4, 2018, pp. 231-5.
Liang FH, Hou XR, Li LH, et al. [Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly by Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu. 2018;43(4):231-5.
Liang, F. H., Hou, X. R., Li, L. H., Liang, X., Lu, Y. W., Yang, H., & Zhang, Y. (2018). [Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly by Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu = Acupuncture Research, 43(4), pp. 231-5. doi:10.13702/j.1000-0607.170791.
Liang FH, et al. [Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly By Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats]. Zhen Ci Yan Jiu. 2018 Apr 25;43(4):231-5. PubMed PMID: 29888576.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Acupoint Injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) May Relieve Nasal Allergic Symptoms Possibly by Down-regulating Expression of Histamine Receptor H 1 and H 4 in Nasal Mucosa of Allergic Rhinitis Rats]. AU - Liang,Fei-Hong, AU - Hou,Xun-Rui, AU - Li,Li-Hong, AU - Liang,Xin, AU - Lu,Yu-Wei, AU - Yang,Hong, AU - Zhang,Yuan, PY - 2018/6/12/entrez PY - 2018/6/12/pubmed PY - 2019/4/4/medline KW - Acupoint injection KW - Allergic rhinitis KW - Histamine H 1 receptor KW - Histamine H 4 receptor KW - Nasal allergic symptoms SP - 231 EP - 5 JF - Zhen ci yan jiu = Acupuncture research JO - Zhen Ci Yan Jiu VL - 43 IS - 4 N2 - OBJECTIVE: To observe the effect of acupoint injection at "Yingxiang"(LI 20) and "Yintang"(GV 29) on nasal allergic reactions and the expression of histamine receptor H 1 and H 4 in nasal mucosa of allergic rhinitis (AR) rats, so as to reveal its mechanism underlying improvement of AR. METHODS: SD rats were randomized into normal, model, non-acupoint injection and acupoint injection groups (n=8 in each). The AR model was established by intraperitoneal (i.p.) injection of mixture of ovalbumin, aluminium hydroxide gel and normal saline (once every other day, for 7 times), and nasal drip of ovalbumin (on the following day of i.p.for 7 days). The mixture solution of lidocaine, dexamethasone (DXM) and transfer factor (0.1 mL/acupoint or non-acupoint) was injected into bilateral LI 20 and GV 29 in the acupoint injection group, or into the non-acupoints (about 5 cm below the armpit on both sides, and the middle point between the left "Houhai"[GV 1] and "Huantiao"[GB 30]), on the 1st , 5th, 9th, and 13th day after modeling. Symptoms of sneezing, nasal discharge, nose-rubbing, etc. were scored after the treatment. The expression levels of H1 R and H4 R proteins and genes in the nasal mucosa tissue were determined using immunohistochemistry and quantitative real-time PCR, respectively. RESULTS: Compared with the normal group, the symptom score and the expression levels of H1 R, H4 R proteins and genes in nasal mucosa were significantly increased in the model group (P<0.05). After acupoint injection for 4 times, the symptom score and the expression levels of H1 R, H4 R proteins and mRNAs were significantly down-regulated in the acupoint injection group relevant to the model group (P<0.05). There were no significant differences between the non-acupoint and the model groups in the abovementioned indexes (P>0.05), suggesting a specificity of the effect of acupoint injection. CONCLUSION: Acupoint injection can relieve the allergic symptoms of AR rats, which may be related to its effects in down-regulating the over expression of H1 R and H4 R proteins and genes in the nasal mucosa. SN - 1000-0607 UR - https://www.unboundmedicine.com/medline/citation/29888576/[Acupoint_Injection_at_"Yingxiang"_LI_20__and_"Yintang"_GV_29__May_Relieve_Nasal_Allergic_Symptoms_Possibly_by_Down_regulating_Expression_of_Histamine_Receptor_H_1_and_H_4_in_Nasal_Mucosa_of_Allergic_Rhinitis_Rats]_ DB - PRIME DP - Unbound Medicine ER -