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Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity.
Acta Biol Hung. 2018 Jun; 69(2):156-169.AB

Abstract

In this study, we analyzed extracts of Ribes (black currant, red currant and gooseberry) fruits obtained with methanol, methanol 50% and water. For each extract total polyphenol content, total flavonoid content and total anthocyanin content was assessed. The antioxidant activity of extracts was evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity and by the photo-chemiluminescence (PCL) method. Identification and quantification of individual phenolic compounds was performed by means of high performance liquid chromatograph coupled with diode array detector (HPLC-DAD) analyses. From each fruit, best extraction of polyphenols was obtained with methanol 50%. In case of red currants and gooseberry there was no significant difference in flavonoids and anthocyanins extraction rate by the different extraction solvents. For black currants the methanol and methanol 50% extract presented the highest antioxidant activity. For red currants extracts with methanol 50% showed stronger antioxidant activity (IC50 = 5.71 mg/ml for DPPH, IC50 = 1.17 mg/ml for ABTS) than those with methanol or water. In case of gooseberry by the DPPH test the water extract proved to be the most active (IC50 = 5.9 mg/ml). In the PCL test black currants methanol 50% extract was over 6 times more powerful as the ones from red currants. In case of gooseberries, water extract presented the highest antioxidant activity (41.84 μmol AAE/g). In black currant cyanidin-3-glucoside was the major compound. Quercetin 3-O-glucoside was identified in each sample. From cinnamic acid derivatives neochlorogenic acid was present in black currants in the highest amount (356.33 μg/g).

Authors+Show Affiliations

1 Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.2 Faculty of Pharmacy, University of Medicine and Pharmacy of Târgu Mureş , student.2 Faculty of Pharmacy, University of Medicine and Pharmacy of Târgu Mureş , student.3 Department of Toxicology and Biopharmacy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.3 Department of Toxicology and Biopharmacy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.3 Department of Toxicology and Biopharmacy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.4 Department of Pharmaceutical Botany, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.1 Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.1 Department of Pharmacognosy and Phytotherapy, University of Medicine and Pharmacy of Târgu Mureş , Gh. Marinescu nr. 38, 540139 Târgu Mureş , Romania.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

29888668

Citation

Laczkó-Zöld, Eszter, et al. "Extractability of Polyphenols From Black Currant, Red Currant and Gooseberry and Their Antioxidant Activity." Acta Biologica Hungarica, vol. 69, no. 2, 2018, pp. 156-169.
Laczkó-Zöld E, Komlósi A, Ülkei T, et al. Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity. Acta Biol Hung. 2018;69(2):156-169.
Laczkó-Zöld, E., Komlósi, A., Ülkei, T., Fogarasi, E., Croitoru, M., Fülöp, I., Domokos, E., Ştefănescu, R., & Varga, E. (2018). Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity. Acta Biologica Hungarica, 69(2), 156-169. https://doi.org/10.1556/018.69.2018.2.5
Laczkó-Zöld E, et al. Extractability of Polyphenols From Black Currant, Red Currant and Gooseberry and Their Antioxidant Activity. Acta Biol Hung. 2018;69(2):156-169. PubMed PMID: 29888668.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Extractability of polyphenols from black currant, red currant and gooseberry and their antioxidant activity. AU - Laczkó-Zöld,Eszter, AU - Komlósi,Andrea, AU - Ülkei,Timea, AU - Fogarasi,Erzsébet, AU - Croitoru,Mircea, AU - Fülöp,Ibolya, AU - Domokos,Erzsébet, AU - Ştefănescu,Ruxandra, AU - Varga,Erzsébet, PY - 2018/6/12/entrez PY - 2018/6/12/pubmed PY - 2018/9/12/medline KW - Ribes nigrum L. KW - Ribes rubrum L. KW - Ribes uva-crispa L. KW - antioxidant activity KW - polyphenols SP - 156 EP - 169 JF - Acta biologica Hungarica JO - Acta Biol Hung VL - 69 IS - 2 N2 - In this study, we analyzed extracts of Ribes (black currant, red currant and gooseberry) fruits obtained with methanol, methanol 50% and water. For each extract total polyphenol content, total flavonoid content and total anthocyanin content was assessed. The antioxidant activity of extracts was evaluated by 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity and by the photo-chemiluminescence (PCL) method. Identification and quantification of individual phenolic compounds was performed by means of high performance liquid chromatograph coupled with diode array detector (HPLC-DAD) analyses. From each fruit, best extraction of polyphenols was obtained with methanol 50%. In case of red currants and gooseberry there was no significant difference in flavonoids and anthocyanins extraction rate by the different extraction solvents. For black currants the methanol and methanol 50% extract presented the highest antioxidant activity. For red currants extracts with methanol 50% showed stronger antioxidant activity (IC50 = 5.71 mg/ml for DPPH, IC50 = 1.17 mg/ml for ABTS) than those with methanol or water. In case of gooseberry by the DPPH test the water extract proved to be the most active (IC50 = 5.9 mg/ml). In the PCL test black currants methanol 50% extract was over 6 times more powerful as the ones from red currants. In case of gooseberries, water extract presented the highest antioxidant activity (41.84 μmol AAE/g). In black currant cyanidin-3-glucoside was the major compound. Quercetin 3-O-glucoside was identified in each sample. From cinnamic acid derivatives neochlorogenic acid was present in black currants in the highest amount (356.33 μg/g). SN - 0236-5383 UR - https://www.unboundmedicine.com/medline/citation/29888668/Extractability_of_polyphenols_from_black_currant_red_currant_and_gooseberry_and_their_antioxidant_activity_ L2 - https://akjournals.com/doi/10.1556/018.69.2018.2.5 DB - PRIME DP - Unbound Medicine ER -